Protein composition and activation markers in plasma collected by three apheresis procedures

Thierry Burnouf, Claudia Kappelsberger, Kerstin Frank, Thomas Burkhardt

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

BACKGROUND: Scientific and technical advances made in transfusion medicine sustain the need for more comprehensive understanding of the impact of collection procedures on the quality of plasma for fractionation and for transfusion. This prospective work evaluated protein composition and markers of activation in plasma donations collected with three different automatic collection procedures (performed on Haemonetics machines), including a new procedure using a high-separation core-molded bowl. STUDY DESIGN AND METHODS: A total of 90 collection procedures have been performed from a population of 37 donors, under comprehensively standardized conditions. Plasma aliquots were taken from the plasma units within 30 minutes of the end of the collection procedures and immediately frozen at -70°C. Content in an extended range of proteins and of markers of activation of the coagulation and fibrinolytic systems has been measured using standard in vitro testing methods. RESULTS: Plasma donations had normal mean total protein, IgG, IgM, and fibrinogen content. The mean levels in coagulation FV, FVII, FVIII, and FXI and in antithrombin were above the standard international requirements. There was no sign of activation of the hemostasis system, as assessed by activated FVII, thrombin antithrombin complex, Prothrombin fragment 1+2, and D-dimers. Activated complement component C3 and C5 were low. CONCLUSION: Data indicates the good and consistent protein composition of plasma obtained by those automatic apheresis procedures. In particular, the new high-separation core procedure yields a high-quality plasma meeting requirements for transfusion and fractionation.

Original languageEnglish
Pages (from-to)1223-1229
Number of pages7
JournalTransfusion
Volume43
Issue number9
DOIs
Publication statusPublished - Sep 1 2003
Externally publishedYes

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Blood Component Removal
Proteins
Complement C5
Transfusion Medicine
Complement C3
Antithrombins
Hemostasis
Fibrinogen
Immunoglobulin M
Blood Proteins
Immunoglobulin G

ASJC Scopus subject areas

  • Immunology
  • Hematology

Cite this

Protein composition and activation markers in plasma collected by three apheresis procedures. / Burnouf, Thierry; Kappelsberger, Claudia; Frank, Kerstin; Burkhardt, Thomas.

In: Transfusion, Vol. 43, No. 9, 01.09.2003, p. 1223-1229.

Research output: Contribution to journalArticle

Burnouf, Thierry ; Kappelsberger, Claudia ; Frank, Kerstin ; Burkhardt, Thomas. / Protein composition and activation markers in plasma collected by three apheresis procedures. In: Transfusion. 2003 ; Vol. 43, No. 9. pp. 1223-1229.
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