Protective role of wogonin against lipopolysaccharide-induced angiogenesis via VEGFR-2, not VEGFR-1

Chiu Mei Lin, Hang Chang, Yeh Hsu Chen, Shih Yun Li, I. Hsing Wu, Jen Hwey Chiu

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

Wogonin, one of flavonoid derived from particular plants, enriches the property of anti-inflammation. Inflammation-stimulated angiogenesis plays an important role in many pathological diseases, such as rheumatoid arthritis, atherosclerosis, and cancer. The aim of this study was to investigate the suppressive effect of wogonin on lipopolysaccharide (LPS)-induced angiogenesis in human umbilical endothelial cell (HUVEC) cultures. By cell differentiation assays, migration and tube formation activity under LPS treatment were evaluated. Besides, IL-6 neutralizing antibody was added to test the inhibitory effect in the phenotypic alteration. Western blot analysis, ELISA cytokine assay, and quantitative real time-PCR were performed for VEGF, IL-6, VEGF receptors, and IL-6 receptor gene expressions on HUVEC with wogonin treatment. Furthermore, in vivo chorioallantoic membrane (CAM) assay was applied to evaluate the percentage of new vessels formation. The results revealed that wogonin (10- 8-10- 5 M) inhibited LPS-induced angiogenesis in a concentration-dependent manner. The mRNA and protein expressions of VEGF, VEGFR-2, IL-6, and sIL-6Rα were attenuated (P <0.05), but not VEGFR-1. In the LPS-induced CAM model, our data suggested that wogonin (10- 8-10- 5 M) significantly decreased new vessel formation and vascular network (P <0.05). We conclude that wogonin suppresses both in vitro and in vivo LPS-induced angiogenesis, through VEGFR-2, but not VEGFR-1.

Original languageEnglish
Pages (from-to)1690-1698
Number of pages9
JournalInternational Immunopharmacology
Volume6
Issue number11
DOIs
Publication statusPublished - Nov 2006

Keywords

  • IL-6
  • Lipopolysaccharide
  • VEGF
  • VEGFR-2
  • Wogonin

ASJC Scopus subject areas

  • Immunology
  • Pharmacology

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