Protective effects of water extract of clam on normal and CCl 4-induced damage in primary cultured rat hepatocytes

H. M. Chi, S. T. Chou, S. C. Lin, Z. Y. Su, Lee Yan Sheen

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The objective of this study was to investigate the effects of various concentrations and incubation times of water extract of clam (WEC) on glutathione, its antioxidant and the detoxification defense systems in normal and CCl4-induced oxidative damaged primary rat hepatocytes. This study showed that when the hepatocytes were treated with WEC(0.14 ∼ 1.68 mg/ml), the intracellular glutathione (GSH) levels, GSH/GSSG ratio, and the activities of GSH-related enzymes (GPx, GRd, and GST) were higher than those in the control at 24 or 48 hour treatments. However, the lactate dehydrogenase (LDH) leakage and microscopic observations did not differ from those of the control. Yet, when the hepatocytes were pretreated with various concentrations of WEC for 24 hours and then exposed to 5 mM carbon tetrachloride (CCl 4) for 1 hour, at concentrations of WEC between 0.42 ∼ 1.68 mg/ml, the viabilities, intracellular GSH level, and activities of GST and GPx were significantly increased compared to those of the CCl4-treated control group (p <0.05). In conclusion, WEC could improve the viability and the capabilities of detoxification and antioxidation in hepatocytes by increasing the GSH level and the activities of GSH-related enzymes.

Original languageEnglish
Pages (from-to)1193-1205
Number of pages13
JournalAmerican Journal of Chinese Medicine
Volume38
Issue number6
DOIs
Publication statusPublished - 2010

Fingerprint

Bivalvia
Hepatocytes
Water
Glutathione
Glutathione Disulfide
Carbon Tetrachloride
Enzymes
L-Lactate Dehydrogenase
Antioxidants
Control Groups

Keywords

  • Carbon Tetrachloride
  • Clam
  • Glutathione
  • Glutathione- Related Enzymes
  • Primary Rat Hepatocytes

ASJC Scopus subject areas

  • Complementary and alternative medicine

Cite this

Protective effects of water extract of clam on normal and CCl 4-induced damage in primary cultured rat hepatocytes. / Chi, H. M.; Chou, S. T.; Lin, S. C.; Su, Z. Y.; Sheen, Lee Yan.

In: American Journal of Chinese Medicine, Vol. 38, No. 6, 2010, p. 1193-1205.

Research output: Contribution to journalArticle

Chi, H. M. ; Chou, S. T. ; Lin, S. C. ; Su, Z. Y. ; Sheen, Lee Yan. / Protective effects of water extract of clam on normal and CCl 4-induced damage in primary cultured rat hepatocytes. In: American Journal of Chinese Medicine. 2010 ; Vol. 38, No. 6. pp. 1193-1205.
@article{73baf570525a4697bd3e2c2d34e8c5ab,
title = "Protective effects of water extract of clam on normal and CCl 4-induced damage in primary cultured rat hepatocytes",
abstract = "The objective of this study was to investigate the effects of various concentrations and incubation times of water extract of clam (WEC) on glutathione, its antioxidant and the detoxification defense systems in normal and CCl4-induced oxidative damaged primary rat hepatocytes. This study showed that when the hepatocytes were treated with WEC(0.14 ∼ 1.68 mg/ml), the intracellular glutathione (GSH) levels, GSH/GSSG ratio, and the activities of GSH-related enzymes (GPx, GRd, and GST) were higher than those in the control at 24 or 48 hour treatments. However, the lactate dehydrogenase (LDH) leakage and microscopic observations did not differ from those of the control. Yet, when the hepatocytes were pretreated with various concentrations of WEC for 24 hours and then exposed to 5 mM carbon tetrachloride (CCl 4) for 1 hour, at concentrations of WEC between 0.42 ∼ 1.68 mg/ml, the viabilities, intracellular GSH level, and activities of GST and GPx were significantly increased compared to those of the CCl4-treated control group (p <0.05). In conclusion, WEC could improve the viability and the capabilities of detoxification and antioxidation in hepatocytes by increasing the GSH level and the activities of GSH-related enzymes.",
keywords = "Carbon Tetrachloride, Clam, Glutathione, Glutathione- Related Enzymes, Primary Rat Hepatocytes",
author = "Chi, {H. M.} and Chou, {S. T.} and Lin, {S. C.} and Su, {Z. Y.} and Sheen, {Lee Yan}",
year = "2010",
doi = "10.1142/S0192415X10008561",
language = "English",
volume = "38",
pages = "1193--1205",
journal = "American Journal of Chinese Medicine",
issn = "0192-415X",
publisher = "World Scientific Publishing Co. Pte Ltd",
number = "6",

}

TY - JOUR

T1 - Protective effects of water extract of clam on normal and CCl 4-induced damage in primary cultured rat hepatocytes

AU - Chi, H. M.

AU - Chou, S. T.

AU - Lin, S. C.

AU - Su, Z. Y.

AU - Sheen, Lee Yan

PY - 2010

Y1 - 2010

N2 - The objective of this study was to investigate the effects of various concentrations and incubation times of water extract of clam (WEC) on glutathione, its antioxidant and the detoxification defense systems in normal and CCl4-induced oxidative damaged primary rat hepatocytes. This study showed that when the hepatocytes were treated with WEC(0.14 ∼ 1.68 mg/ml), the intracellular glutathione (GSH) levels, GSH/GSSG ratio, and the activities of GSH-related enzymes (GPx, GRd, and GST) were higher than those in the control at 24 or 48 hour treatments. However, the lactate dehydrogenase (LDH) leakage and microscopic observations did not differ from those of the control. Yet, when the hepatocytes were pretreated with various concentrations of WEC for 24 hours and then exposed to 5 mM carbon tetrachloride (CCl 4) for 1 hour, at concentrations of WEC between 0.42 ∼ 1.68 mg/ml, the viabilities, intracellular GSH level, and activities of GST and GPx were significantly increased compared to those of the CCl4-treated control group (p <0.05). In conclusion, WEC could improve the viability and the capabilities of detoxification and antioxidation in hepatocytes by increasing the GSH level and the activities of GSH-related enzymes.

AB - The objective of this study was to investigate the effects of various concentrations and incubation times of water extract of clam (WEC) on glutathione, its antioxidant and the detoxification defense systems in normal and CCl4-induced oxidative damaged primary rat hepatocytes. This study showed that when the hepatocytes were treated with WEC(0.14 ∼ 1.68 mg/ml), the intracellular glutathione (GSH) levels, GSH/GSSG ratio, and the activities of GSH-related enzymes (GPx, GRd, and GST) were higher than those in the control at 24 or 48 hour treatments. However, the lactate dehydrogenase (LDH) leakage and microscopic observations did not differ from those of the control. Yet, when the hepatocytes were pretreated with various concentrations of WEC for 24 hours and then exposed to 5 mM carbon tetrachloride (CCl 4) for 1 hour, at concentrations of WEC between 0.42 ∼ 1.68 mg/ml, the viabilities, intracellular GSH level, and activities of GST and GPx were significantly increased compared to those of the CCl4-treated control group (p <0.05). In conclusion, WEC could improve the viability and the capabilities of detoxification and antioxidation in hepatocytes by increasing the GSH level and the activities of GSH-related enzymes.

KW - Carbon Tetrachloride

KW - Clam

KW - Glutathione

KW - Glutathione- Related Enzymes

KW - Primary Rat Hepatocytes

UR - http://www.scopus.com/inward/record.url?scp=78149418114&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=78149418114&partnerID=8YFLogxK

U2 - 10.1142/S0192415X10008561

DO - 10.1142/S0192415X10008561

M3 - Article

VL - 38

SP - 1193

EP - 1205

JO - American Journal of Chinese Medicine

JF - American Journal of Chinese Medicine

SN - 0192-415X

IS - 6

ER -