Protective effects of oral astaxanthin nanopowder against ultraviolet-induced photokeratitis in mice

Fumiya Harada, Tetsuro Morikawa, Anton Lennikov, Anthony Mukwaya, Mira Schaupper, Osamu Uehara, Rie Takai, Koki Yoshida, Jun Sato, Yukihiro Horie, Hiroyuki Sakaguchi, Ching Zong Wu, Yoshihiro Abiko, Neil Lagali, Nobuyoshi Kitaichi

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Purpose. Astaxanthin (AST) has a strong antioxidant cellular membrane chaperone protective effect. Recently, a water-soluble nanosized AST (nano-AST) form was produced, which is expected to improve the efficacy of oral intake effects. The purpose of this study was to examine whether oral nano-AST has therapeutic effects on UV-induced photokeratitis in mice. Methods. C57BL/6 mice were administered twice with either nano-AST, AST oil, lutein, or bilberry extracts 3 hours before and shortly before UV irradiation (dose: 400 mJ/cm2). The corneas were collected 24 hours after irradiation and stained with H&E and TUNEL. NF-κB, dihydroethidium (DHE), COX-2, p-IκB-α, TNFα, and CD45 expression were evaluated through immunohistochemistry, Western blot analysis, and qPCR. Results. Corneal epithelium was significantly thicker in mice orally administered with nano-AST than in the others (p<0.01), with significantly less NF-κB nucleus translocation (p<0.001), and significantly fewer TUNEL cells (p<0.01). Weaker DHE signals were detected in the nano-AST group (p<0.05) relative to the others. Furthermore, reduced inflammation and decreased cell death in corneal tissue were observed in the nano-AST group, as indicated by a reduction in the expression of COX-2, p-IκB-α, TNFα, and CD45. Conclusions. Oral administration of nano-AST demonstrated a protective effect on UV-induced photokeratitis via antioxidative, anti-inflammatory, and antiapoptotic activity.

Original languageEnglish
Article number1956104
JournalOxidative Medicine and Cellular Longevity
Volume2017
DOIs
Publication statusPublished - Jan 1 2017

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In Situ Nick-End Labeling
Vaccinium myrtillus
Irradiation
Lutein
Corneal Epithelium
Therapeutic Uses
Cell death
Inbred C57BL Mouse
Cornea
Dosimetry
Oral Administration
Oils
Cell Death
Anti-Inflammatory Agents
Antioxidants
Western Blotting
Immunohistochemistry
Tissue
Inflammation
Membranes

ASJC Scopus subject areas

  • Biochemistry
  • Ageing
  • Cell Biology

Cite this

Harada, F., Morikawa, T., Lennikov, A., Mukwaya, A., Schaupper, M., Uehara, O., ... Kitaichi, N. (2017). Protective effects of oral astaxanthin nanopowder against ultraviolet-induced photokeratitis in mice. Oxidative Medicine and Cellular Longevity, 2017, [1956104]. https://doi.org/10.1155/2017/1956104

Protective effects of oral astaxanthin nanopowder against ultraviolet-induced photokeratitis in mice. / Harada, Fumiya; Morikawa, Tetsuro; Lennikov, Anton; Mukwaya, Anthony; Schaupper, Mira; Uehara, Osamu; Takai, Rie; Yoshida, Koki; Sato, Jun; Horie, Yukihiro; Sakaguchi, Hiroyuki; Wu, Ching Zong; Abiko, Yoshihiro; Lagali, Neil; Kitaichi, Nobuyoshi.

In: Oxidative Medicine and Cellular Longevity, Vol. 2017, 1956104, 01.01.2017.

Research output: Contribution to journalArticle

Harada, F, Morikawa, T, Lennikov, A, Mukwaya, A, Schaupper, M, Uehara, O, Takai, R, Yoshida, K, Sato, J, Horie, Y, Sakaguchi, H, Wu, CZ, Abiko, Y, Lagali, N & Kitaichi, N 2017, 'Protective effects of oral astaxanthin nanopowder against ultraviolet-induced photokeratitis in mice', Oxidative Medicine and Cellular Longevity, vol. 2017, 1956104. https://doi.org/10.1155/2017/1956104
Harada, Fumiya ; Morikawa, Tetsuro ; Lennikov, Anton ; Mukwaya, Anthony ; Schaupper, Mira ; Uehara, Osamu ; Takai, Rie ; Yoshida, Koki ; Sato, Jun ; Horie, Yukihiro ; Sakaguchi, Hiroyuki ; Wu, Ching Zong ; Abiko, Yoshihiro ; Lagali, Neil ; Kitaichi, Nobuyoshi. / Protective effects of oral astaxanthin nanopowder against ultraviolet-induced photokeratitis in mice. In: Oxidative Medicine and Cellular Longevity. 2017 ; Vol. 2017.
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abstract = "Purpose. Astaxanthin (AST) has a strong antioxidant cellular membrane chaperone protective effect. Recently, a water-soluble nanosized AST (nano-AST) form was produced, which is expected to improve the efficacy of oral intake effects. The purpose of this study was to examine whether oral nano-AST has therapeutic effects on UV-induced photokeratitis in mice. Methods. C57BL/6 mice were administered twice with either nano-AST, AST oil, lutein, or bilberry extracts 3 hours before and shortly before UV irradiation (dose: 400 mJ/cm2). The corneas were collected 24 hours after irradiation and stained with H&E and TUNEL. NF-κB, dihydroethidium (DHE), COX-2, p-IκB-α, TNFα, and CD45 expression were evaluated through immunohistochemistry, Western blot analysis, and qPCR. Results. Corneal epithelium was significantly thicker in mice orally administered with nano-AST than in the others (p<0.01), with significantly less NF-κB nucleus translocation (p<0.001), and significantly fewer TUNEL cells (p<0.01). Weaker DHE signals were detected in the nano-AST group (p<0.05) relative to the others. Furthermore, reduced inflammation and decreased cell death in corneal tissue were observed in the nano-AST group, as indicated by a reduction in the expression of COX-2, p-IκB-α, TNFα, and CD45. Conclusions. Oral administration of nano-AST demonstrated a protective effect on UV-induced photokeratitis via antioxidative, anti-inflammatory, and antiapoptotic activity.",
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AU - Morikawa, Tetsuro

AU - Lennikov, Anton

AU - Mukwaya, Anthony

AU - Schaupper, Mira

AU - Uehara, Osamu

AU - Takai, Rie

AU - Yoshida, Koki

AU - Sato, Jun

AU - Horie, Yukihiro

AU - Sakaguchi, Hiroyuki

AU - Wu, Ching Zong

AU - Abiko, Yoshihiro

AU - Lagali, Neil

AU - Kitaichi, Nobuyoshi

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