Protective effects of melatonin on myocardial ischemia/reperfusion injury in vivo

Yen Mei Lee, Huey Rue Chen, George Hsiao, Joen Rong Sheu, Jhi Joung Wang, Mao Hsiung Yen

Research output: Contribution to journalArticle

81 Citations (Scopus)

Abstract

The production of oxygen free radicals has been strongly implicated as an important pathophysiological mechanism mediating myocardial ischemia/reperfusion (I/R) injury. Various antioxidants have cardioprotective effects. Melatonin, an indoleamine synthesized by the pineal gland, is a potent antioxidant and a direct free radical scavenger. This is the first in vivo study to evaluate the effect of melatonin (0.5, 1.0, and 5.0 mg/kg, i.v. bolus) on myocardial I/R injury in anesthetized Sprague-Dawley rats. Results demonstrate that pretreatment with intermediate or high doses of melatonin (1.0 and 5.0 mg/kg) at 10 min before left coronary artery occlusion markedly suppressed ventricular tachycardia (VT) and ventricular fibrillation (VF), while reducing the total number of premature ventricular contractions and total duration of VT and VF that occurred during the 45-min ischemic period. Pretreatment with melatonin dramatically improved survival rate of rats when compared with the I/R-only group. After 1-hr reperfusion, melatonin caused a significant reduction in infarct size when compared with I/R-only group. Meanwhile, pretreatment with melatonin (1.0 mg/kg) significantly reduced superoxide anion production and myeloperoxidase activity; the latter is an index of neutrophil infiltration in the ischemic myocardium. Additionally, pretreatment with melatonin (1.0 and 5.0 mg/kg) significantly attenuated ventricular arrhythmias and mortality elicited by reperfusion following 5-min ischemia. In conclusion, melatonin suppresses ischemia- and reperfusion-induced ventricular arrhythmias and reduces infarct size resulting from I/R injury. The pronounced cardioprotective activity of melatonin may be mediated by its antioxidant activity and by its capacity for neutrophil inhibition in myocardial I/R.

Original languageEnglish
Pages (from-to)72-80
Number of pages9
JournalJournal of Pineal Research
Volume33
Issue number2
DOIs
Publication statusPublished - 2002

Fingerprint

Myocardial Reperfusion Injury
Melatonin
Reperfusion Injury
Myocardial Ischemia
Reperfusion
Ischemia
Antioxidants
Ventricular Fibrillation
Ventricular Tachycardia
Cardiac Arrhythmias
Free Radical Scavengers
Pineal Gland
Ventricular Premature Complexes
Neutrophil Infiltration
Coronary Occlusion
Superoxides
Peroxidase
Free Radicals
Sprague Dawley Rats
Reactive Oxygen Species

Keywords

  • Antioxidant
  • Arrhythmia
  • Infarction
  • Melatonin
  • Myocardial ischemia
  • Reperfusion injury

ASJC Scopus subject areas

  • Endocrinology

Cite this

Protective effects of melatonin on myocardial ischemia/reperfusion injury in vivo. / Lee, Yen Mei; Chen, Huey Rue; Hsiao, George; Sheu, Joen Rong; Wang, Jhi Joung; Yen, Mao Hsiung.

In: Journal of Pineal Research, Vol. 33, No. 2, 2002, p. 72-80.

Research output: Contribution to journalArticle

Lee, Yen Mei ; Chen, Huey Rue ; Hsiao, George ; Sheu, Joen Rong ; Wang, Jhi Joung ; Yen, Mao Hsiung. / Protective effects of melatonin on myocardial ischemia/reperfusion injury in vivo. In: Journal of Pineal Research. 2002 ; Vol. 33, No. 2. pp. 72-80.
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