Protective effects of Ginkgo biloba, Panax ginseng, and Schizandra chinensis extract on liver injury in rats

Hsin Fang Chang, Yun Ho Lin, Chia Chou Chu, Shu Ju Wu, Ya Hui Tsai, Jane C J Chao

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

This study investigated the effects of the combined extracts of Ginkgo biloba, Panax ginseng, and Schizandra chinensis at different doses on hepatic antioxidant status and fibrosis in rats with carbon tetrachloride (CCl4)-induced liver injury. Male Sprague-Dawley rats (n = 8-12 per group) were divided into the control, CCl4, CCl4 + silymarin (0.35%), CCl4 + low-dose herbal extract (0.24% of Ginkgo biloba, Panax ginseng, and Schizandra chinensis extract at 1:1:1; LE), and CCl4 + high-dose herbal extract (1.20% of the same herbal extract; HE) groups. Silymarin or herbal extract was orally given to rats a week before chronic intraperitoneal injection with CCl4 for 6 weeks. The pathological results showed that herbal extract suppressed hepatic bile duct proliferation, and low-dose herbal extract inhibited liver fibrosis. Hepatic superoxide dismutase (SOD) activity was lower in the CCl4 group, but there was no difference in the silymarin or herbal extract treated groups compared to the control group. Hepatic catalase activity and the ratio of reduced to oxidized glutathione were significantly higher (p <0.05) in the HE group than those in the CCl4 group. Silymarin and herbal extract reversed the impaired hepatic total antioxidant status (p <0.05). Herbal extract partially reduced the elevated hepatic lipid peroxides. Hepatic transforming growth factor-β1 (TGF-β1) level decreased significantly (p <0.05) in the LE group. Therefore, high-dose herbal extract improved hepatic antioxidant capacity through enhancing catalase activity and glutathione redox status, whereas low-dose herbal extract inhibited liver fibrosis through decreasing hepatic TGF-β1 level in rats with CCl4-induced liver injury.

Original languageEnglish
Pages (from-to)995-1009
Number of pages15
JournalAmerican Journal of Chinese Medicine
Volume35
Issue number6
DOIs
Publication statusPublished - 2007

Fingerprint

Schisandra
Ginkgo biloba
Liver Extracts
Panax
Liver
Wounds and Injuries
Silymarin
Antioxidants
Transforming Growth Factors
Liver Cirrhosis
Catalase
Common Hepatic Duct
Glutathione Disulfide
Carbon Tetrachloride
Lipid Peroxides
Bile Ducts
Intraperitoneal Injections
Superoxide Dismutase
Oxidation-Reduction
Glutathione

Keywords

  • Ginkgo biloba
  • Liver Injury
  • Panax ginseng
  • Rat
  • Schizandra chinensis

ASJC Scopus subject areas

  • Complementary and alternative medicine

Cite this

Protective effects of Ginkgo biloba, Panax ginseng, and Schizandra chinensis extract on liver injury in rats. / Chang, Hsin Fang; Lin, Yun Ho; Chu, Chia Chou; Wu, Shu Ju; Tsai, Ya Hui; Chao, Jane C J.

In: American Journal of Chinese Medicine, Vol. 35, No. 6, 2007, p. 995-1009.

Research output: Contribution to journalArticle

@article{efecf98fd65a41cc8c514324bbdc7ed1,
title = "Protective effects of Ginkgo biloba, Panax ginseng, and Schizandra chinensis extract on liver injury in rats",
abstract = "This study investigated the effects of the combined extracts of Ginkgo biloba, Panax ginseng, and Schizandra chinensis at different doses on hepatic antioxidant status and fibrosis in rats with carbon tetrachloride (CCl4)-induced liver injury. Male Sprague-Dawley rats (n = 8-12 per group) were divided into the control, CCl4, CCl4 + silymarin (0.35{\%}), CCl4 + low-dose herbal extract (0.24{\%} of Ginkgo biloba, Panax ginseng, and Schizandra chinensis extract at 1:1:1; LE), and CCl4 + high-dose herbal extract (1.20{\%} of the same herbal extract; HE) groups. Silymarin or herbal extract was orally given to rats a week before chronic intraperitoneal injection with CCl4 for 6 weeks. The pathological results showed that herbal extract suppressed hepatic bile duct proliferation, and low-dose herbal extract inhibited liver fibrosis. Hepatic superoxide dismutase (SOD) activity was lower in the CCl4 group, but there was no difference in the silymarin or herbal extract treated groups compared to the control group. Hepatic catalase activity and the ratio of reduced to oxidized glutathione were significantly higher (p <0.05) in the HE group than those in the CCl4 group. Silymarin and herbal extract reversed the impaired hepatic total antioxidant status (p <0.05). Herbal extract partially reduced the elevated hepatic lipid peroxides. Hepatic transforming growth factor-β1 (TGF-β1) level decreased significantly (p <0.05) in the LE group. Therefore, high-dose herbal extract improved hepatic antioxidant capacity through enhancing catalase activity and glutathione redox status, whereas low-dose herbal extract inhibited liver fibrosis through decreasing hepatic TGF-β1 level in rats with CCl4-induced liver injury.",
keywords = "Ginkgo biloba, Liver Injury, Panax ginseng, Rat, Schizandra chinensis",
author = "Chang, {Hsin Fang} and Lin, {Yun Ho} and Chu, {Chia Chou} and Wu, {Shu Ju} and Tsai, {Ya Hui} and Chao, {Jane C J}",
year = "2007",
doi = "10.1142/S0192415X07005466",
language = "English",
volume = "35",
pages = "995--1009",
journal = "American Journal of Chinese Medicine",
issn = "0192-415X",
publisher = "World Scientific Publishing Co. Pte Ltd",
number = "6",

}

TY - JOUR

T1 - Protective effects of Ginkgo biloba, Panax ginseng, and Schizandra chinensis extract on liver injury in rats

AU - Chang, Hsin Fang

AU - Lin, Yun Ho

AU - Chu, Chia Chou

AU - Wu, Shu Ju

AU - Tsai, Ya Hui

AU - Chao, Jane C J

PY - 2007

Y1 - 2007

N2 - This study investigated the effects of the combined extracts of Ginkgo biloba, Panax ginseng, and Schizandra chinensis at different doses on hepatic antioxidant status and fibrosis in rats with carbon tetrachloride (CCl4)-induced liver injury. Male Sprague-Dawley rats (n = 8-12 per group) were divided into the control, CCl4, CCl4 + silymarin (0.35%), CCl4 + low-dose herbal extract (0.24% of Ginkgo biloba, Panax ginseng, and Schizandra chinensis extract at 1:1:1; LE), and CCl4 + high-dose herbal extract (1.20% of the same herbal extract; HE) groups. Silymarin or herbal extract was orally given to rats a week before chronic intraperitoneal injection with CCl4 for 6 weeks. The pathological results showed that herbal extract suppressed hepatic bile duct proliferation, and low-dose herbal extract inhibited liver fibrosis. Hepatic superoxide dismutase (SOD) activity was lower in the CCl4 group, but there was no difference in the silymarin or herbal extract treated groups compared to the control group. Hepatic catalase activity and the ratio of reduced to oxidized glutathione were significantly higher (p <0.05) in the HE group than those in the CCl4 group. Silymarin and herbal extract reversed the impaired hepatic total antioxidant status (p <0.05). Herbal extract partially reduced the elevated hepatic lipid peroxides. Hepatic transforming growth factor-β1 (TGF-β1) level decreased significantly (p <0.05) in the LE group. Therefore, high-dose herbal extract improved hepatic antioxidant capacity through enhancing catalase activity and glutathione redox status, whereas low-dose herbal extract inhibited liver fibrosis through decreasing hepatic TGF-β1 level in rats with CCl4-induced liver injury.

AB - This study investigated the effects of the combined extracts of Ginkgo biloba, Panax ginseng, and Schizandra chinensis at different doses on hepatic antioxidant status and fibrosis in rats with carbon tetrachloride (CCl4)-induced liver injury. Male Sprague-Dawley rats (n = 8-12 per group) were divided into the control, CCl4, CCl4 + silymarin (0.35%), CCl4 + low-dose herbal extract (0.24% of Ginkgo biloba, Panax ginseng, and Schizandra chinensis extract at 1:1:1; LE), and CCl4 + high-dose herbal extract (1.20% of the same herbal extract; HE) groups. Silymarin or herbal extract was orally given to rats a week before chronic intraperitoneal injection with CCl4 for 6 weeks. The pathological results showed that herbal extract suppressed hepatic bile duct proliferation, and low-dose herbal extract inhibited liver fibrosis. Hepatic superoxide dismutase (SOD) activity was lower in the CCl4 group, but there was no difference in the silymarin or herbal extract treated groups compared to the control group. Hepatic catalase activity and the ratio of reduced to oxidized glutathione were significantly higher (p <0.05) in the HE group than those in the CCl4 group. Silymarin and herbal extract reversed the impaired hepatic total antioxidant status (p <0.05). Herbal extract partially reduced the elevated hepatic lipid peroxides. Hepatic transforming growth factor-β1 (TGF-β1) level decreased significantly (p <0.05) in the LE group. Therefore, high-dose herbal extract improved hepatic antioxidant capacity through enhancing catalase activity and glutathione redox status, whereas low-dose herbal extract inhibited liver fibrosis through decreasing hepatic TGF-β1 level in rats with CCl4-induced liver injury.

KW - Ginkgo biloba

KW - Liver Injury

KW - Panax ginseng

KW - Rat

KW - Schizandra chinensis

UR - http://www.scopus.com/inward/record.url?scp=37849028016&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=37849028016&partnerID=8YFLogxK

U2 - 10.1142/S0192415X07005466

DO - 10.1142/S0192415X07005466

M3 - Article

C2 - 18186586

AN - SCOPUS:37849028016

VL - 35

SP - 995

EP - 1009

JO - American Journal of Chinese Medicine

JF - American Journal of Chinese Medicine

SN - 0192-415X

IS - 6

ER -