Protective effects of estrogen on ischemia/reperfusion-induced bladder dysfunction in female rabbits

Shu Mien Chuang, Cheng Yu Long, Rong Jyh Lin, Keh Min Liu, Robert M. Levin, Chao Yuan Chang, Ya Wen Ho, Wen Jeng Wu, Wei Chiao Chang, Yung Shun Juan

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Objective: The present study investigated the effects of ovarian hormone depletion and estrogen administration on ischemia/reperfusion (I/R)-induced bladder damage in female rabbits. Methods: Female New Zealand white rabbits were divided into five groups. A sham surgical procedure was performed on rabbits in group 1. In group 2, both vesical arteries were clamped for 2 hours and then released (I/R surgical procedure). In group 3, 17β-estradiol (100 μg/kg/d) was injected intramuscularly before I/R surgical procedure. In group 4, ovariectomies were performed before I/R surgical procedure. Group 5 had ovariectomy, recovered for 2 weeks, and then received 17β-estradiol for 2 weeks. I/R surgical procedure was performed thereafter. Rabbits were killed 7 days after I/R surgical procedure. Masson's trichrome stain was used, and immunohistochemical experiments were performed to evaluate interstitial fibrosis and intramural nerve changes. Western immunoblots were examined to determine the expressions of markers for inflammation, fibrosis, and oxidative stress. Results: I/R surgical procedure decreased bladder contractile responses by 30% to 50%. Ovarian hormone depletion further reduced bladder contractile function by 45% to 55% compared with the I/R group members that retained their ovaries. Moreover, I/R surgical procedure significantly decreased intramural neurofilament staining by two thirds compared with the control group. Estrogen replacement after ovariectomy significantly increased the density of nerve terminals. In addition, the expression of transforming growth factor-β and fibronectin increased twofold and fivefold after I/R, respectively. Ovarian hormone depletion further increased the expression of these inflammatory and fibrosis markers. Ovariectomy significantly exacerbated oxidative damage, whereas estrogen replacement diminished oxidative stress to a level approaching that of the control group. Conclusions: I/R surgical procedure increases oxidative damage, enhances interstitial fibrosis, and results in bladder denervation. Ovarian hormone deficiency exacerbates this I/R-induced bladder damage, whereas estrogen therapy after ovariectomy attenuates this injury. These results reveal estrogen's protective effects on bladders subjected to I/R injury and the potential benefits of estrogen therapy on I/R-induced bladder damage.

Original languageEnglish
Pages (from-to)209-217
Number of pages9
JournalMenopause
Volume20
Issue number2
DOIs
Publication statusPublished - Feb 2013

Fingerprint

Reperfusion
Estrogens
Urinary Bladder
Ischemia
Rabbits
Ovariectomy
Fibrosis
Hormones
Estrogen Replacement Therapy
Estradiol
Oxidative Stress
Control Groups
Intermediate Filaments
Transforming Growth Factors
Denervation
Reperfusion Injury
Fibronectins
Ovary
Arteries
Western Blotting

Keywords

  • Bladder
  • Estrogen
  • Ischemia/reperfusion
  • Ovariectomy

ASJC Scopus subject areas

  • Obstetrics and Gynaecology

Cite this

Chuang, S. M., Long, C. Y., Lin, R. J., Liu, K. M., Levin, R. M., Chang, C. Y., ... Juan, Y. S. (2013). Protective effects of estrogen on ischemia/reperfusion-induced bladder dysfunction in female rabbits. Menopause, 20(2), 209-217. https://doi.org/10.1097/gme.0b013e3182635bae

Protective effects of estrogen on ischemia/reperfusion-induced bladder dysfunction in female rabbits. / Chuang, Shu Mien; Long, Cheng Yu; Lin, Rong Jyh; Liu, Keh Min; Levin, Robert M.; Chang, Chao Yuan; Ho, Ya Wen; Wu, Wen Jeng; Chang, Wei Chiao; Juan, Yung Shun.

In: Menopause, Vol. 20, No. 2, 02.2013, p. 209-217.

Research output: Contribution to journalArticle

Chuang, SM, Long, CY, Lin, RJ, Liu, KM, Levin, RM, Chang, CY, Ho, YW, Wu, WJ, Chang, WC & Juan, YS 2013, 'Protective effects of estrogen on ischemia/reperfusion-induced bladder dysfunction in female rabbits', Menopause, vol. 20, no. 2, pp. 209-217. https://doi.org/10.1097/gme.0b013e3182635bae
Chuang, Shu Mien ; Long, Cheng Yu ; Lin, Rong Jyh ; Liu, Keh Min ; Levin, Robert M. ; Chang, Chao Yuan ; Ho, Ya Wen ; Wu, Wen Jeng ; Chang, Wei Chiao ; Juan, Yung Shun. / Protective effects of estrogen on ischemia/reperfusion-induced bladder dysfunction in female rabbits. In: Menopause. 2013 ; Vol. 20, No. 2. pp. 209-217.
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N2 - Objective: The present study investigated the effects of ovarian hormone depletion and estrogen administration on ischemia/reperfusion (I/R)-induced bladder damage in female rabbits. Methods: Female New Zealand white rabbits were divided into five groups. A sham surgical procedure was performed on rabbits in group 1. In group 2, both vesical arteries were clamped for 2 hours and then released (I/R surgical procedure). In group 3, 17β-estradiol (100 μg/kg/d) was injected intramuscularly before I/R surgical procedure. In group 4, ovariectomies were performed before I/R surgical procedure. Group 5 had ovariectomy, recovered for 2 weeks, and then received 17β-estradiol for 2 weeks. I/R surgical procedure was performed thereafter. Rabbits were killed 7 days after I/R surgical procedure. Masson's trichrome stain was used, and immunohistochemical experiments were performed to evaluate interstitial fibrosis and intramural nerve changes. Western immunoblots were examined to determine the expressions of markers for inflammation, fibrosis, and oxidative stress. Results: I/R surgical procedure decreased bladder contractile responses by 30% to 50%. Ovarian hormone depletion further reduced bladder contractile function by 45% to 55% compared with the I/R group members that retained their ovaries. Moreover, I/R surgical procedure significantly decreased intramural neurofilament staining by two thirds compared with the control group. Estrogen replacement after ovariectomy significantly increased the density of nerve terminals. In addition, the expression of transforming growth factor-β and fibronectin increased twofold and fivefold after I/R, respectively. Ovarian hormone depletion further increased the expression of these inflammatory and fibrosis markers. Ovariectomy significantly exacerbated oxidative damage, whereas estrogen replacement diminished oxidative stress to a level approaching that of the control group. Conclusions: I/R surgical procedure increases oxidative damage, enhances interstitial fibrosis, and results in bladder denervation. Ovarian hormone deficiency exacerbates this I/R-induced bladder damage, whereas estrogen therapy after ovariectomy attenuates this injury. These results reveal estrogen's protective effects on bladders subjected to I/R injury and the potential benefits of estrogen therapy on I/R-induced bladder damage.

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