Protective effects of anthocyanins against amyloid β-peptide-induced damage in neuro-2A Cells

Ping Hsiao Shih, Chi Hao Wu, Chi-Tai Yeh, Gow Chin Yen

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Alzheimer's disease is neuropathologically characterized by amyloid β-protein (Aβ) deposition, resulting in neurotoxicity. Herein, we focused on the prevention of anthocyanins from amyloid-mediated neurodysfunction. The data demonstrated that combined exposure of Aβ 1-40 and Aβ 25-35 to Neuro-2A cells resulted in reactive oxygen species (ROS) production and perturbation of calcium homeostasis. The expressions of LXRα, ApoE, ABCA1, and seladin-1 genes were significantly down-regulated upon Aβ challenge. β-Secretase, the rate-limiting enzyme that catalyzes amyloid precursor protein transform to Aβ, was up-regulated by Aβ treatment. For the duration of Aβ stimulation, malvidin (Mal) or oenin (Oen; malvidin-3-O-glucoside) was added, and the protective effects were observed. Mal and Oen showed protective effects against Aβ-induced neurotoxicity through blocking ROS formation, preserving Ca 2+ homeostasis, and preventing Aβ-mediated perturbation of certain genes involved in Aβ metabolism and cellular defense. The present study implicates anthocyanin as a potential therapeutic candidate for the prevention of amyloid-mediated neurodysfunction.

Original languageEnglish
Pages (from-to)1683-1689
Number of pages7
JournalJournal of Agricultural and Food Chemistry
Volume59
Issue number5
DOIs
Publication statusPublished - Mar 9 2011

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Keywords

  • β-secretase
  • Alzheimer's disease
  • amyloid β-protein
  • anthocyanin
  • Neuro-2A cells
  • neuroprotection

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Chemistry(all)

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