Protective effects of Angelica sinensis extract on amyloid β-peptide-induced neurotoxicity

Shih Hao Huang, Chun Mao Lin, Been Huang Chiang

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72 Citations (Scopus)

Abstract

The protective effects of alcohol extract from the root of Angelica sinensis (AS) on β-amyloid peptide (Aβ)-induced toxicity and the mechanism of these effects were investigated. Aβ is a pathological hallmark of Alzheimer's disease; it decreased viability of Neuro 2A cells in a concentration-dependent manner with IC50 of 14.9 μM. AS extract resulted in dose-dependent anti-Aβ toxicity according to MTT assay. Reactive oxygen species (ROS) analysis revealed a significant production of hydrogen peroxide, decreased glutathione (GSH) levels and increased lipid peroxidation (TBARS value) in the Aβ-treated Neuro 2A cells. The Aβ-treated cells also showed a significant decline in the mitochondrial transmembrane potential (ΔΨm) and increase in the mitochondrial volume, and portions of the cytoplasm were sequestered by a membrane-bound vacuole. The malfunctions of Neuro 2A cells caused by Aβ were attenuated using AS extract. The AS extract protected cell viability against Aβ-induced oxidative damage (ROS, TBARS, and GSH contents) and rescued the ΔΨm levels in a dose-dependent manner: the dosages of 25, 50, 100, and 200 μg/ml recovered 77%, 87%, 102%, and 105% of ΔΨm, respectively. AS extract also recovered the enlarged mitochondria mass with dosages from 25 to 200 μg/ml. The results of this study demonstrated that AS extract possessed the activity to prevent the neurotoxicity induced by Aβ-associated oxidative stress, implying that AS has a potential role in the prevention of Alzheimer's diseases.

Original languageEnglish
Pages (from-to)710-721
Number of pages12
JournalPhytomedicine
Volume15
Issue number9
DOIs
Publication statusPublished - Sep 3 2008

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Keywords

  • β-Amyloid peptide
  • Angelica sinensis
  • Glutathione
  • Mitochondria
  • Mitochondrial transmembrane potential (ΔΨm)
  • ROS

ASJC Scopus subject areas

  • Plant Science
  • Drug Discovery
  • Organic Chemistry
  • Pharmacology

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