Protective effects of adiponectin against renal ischemia-reperfusion injury via prostacyclin-PPARα-Heme oxygenase-1 signaling pathway

Ching Feng Cheng, Wei Shiung Lian, Sung Ho Chen, Pei Fen Lai, Hsiao Fen Li, Yi Fan Lan, Winston Teng Kuei Cheng, Heng Lin

Research output: Contribution to journalArticle

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Abstract

Adiponectin (APN), a circulating adipose-derived hormone that regulates inflammation and energy metabolism, has beneficial effects on the cardiovascular disorders. Serum APN levels are lower in patients with coronary artery disease and higher in patients with chronic kidney disease. However, the precise role of APN in acute reno-vascular disease is not clear. Results of the present study show that serum APN concentration decreased after renal ischemia reperfusion (I/R) injury in mice. In addition, I/R-induced renal dysfunction (elevated serum creatinine and urea levels), inflammation (number of infiltrating neutrophils, myeloperoxidase activity), and apoptotic responses (apoptotic cell number and caspase-3 activation) were attenuated in APN-treated compared to control mice. Molecular and biochemical analysis revealed that APN up-regulates heme oxygenase-1 (HO-1) via peroxisome-proliferator-activated-receptor-α (PPARα) dependent pathway which is mediated through the enhancement of COX-2 and 6-keto PGF1α expression. Chromatin immune-precipitation assay demonstrated that APN increases the binding activity of PPARα to PPRE region of HO-1 promoter. Furthermore, APN induced HO-1 expression was only found in wild-type but not in PPARα gene deleted mice. This provides in vivo evidence that APN mediated HO-1 expression depends on PPARα regulation. In conclusion, our results provide a novel APN mediated prostacyclin-PPARα-HO-1 signaling pathway in protecting renal I/R injury.

Original languageEnglish
Pages (from-to)239-249
Number of pages11
JournalJournal of Cellular Physiology
Volume227
Issue number1
DOIs
Publication statusPublished - Jan 2012

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Peroxisome Proliferator-Activated Receptors
Heme Oxygenase-1
Adiponectin
Epoprostenol
Reperfusion Injury
Kidney
Serum
Inflammation
Chronic Renal Insufficiency
Vascular Diseases
Immunoprecipitation
Caspase 3
Energy Metabolism
Peroxidase
Chromatin
Reperfusion
Urea
Coronary Artery Disease
Assays
Creatinine

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology
  • Physiology

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Protective effects of adiponectin against renal ischemia-reperfusion injury via prostacyclin-PPARα-Heme oxygenase-1 signaling pathway. / Cheng, Ching Feng; Lian, Wei Shiung; Chen, Sung Ho; Lai, Pei Fen; Li, Hsiao Fen; Lan, Yi Fan; Cheng, Winston Teng Kuei; Lin, Heng.

In: Journal of Cellular Physiology, Vol. 227, No. 1, 01.2012, p. 239-249.

Research output: Contribution to journalArticle

Cheng, Ching Feng ; Lian, Wei Shiung ; Chen, Sung Ho ; Lai, Pei Fen ; Li, Hsiao Fen ; Lan, Yi Fan ; Cheng, Winston Teng Kuei ; Lin, Heng. / Protective effects of adiponectin against renal ischemia-reperfusion injury via prostacyclin-PPARα-Heme oxygenase-1 signaling pathway. In: Journal of Cellular Physiology. 2012 ; Vol. 227, No. 1. pp. 239-249.
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