Protective effects and network analysis of natural compounds obtained from Radix dipsaci, Eucommiae cortex, and Rhizoma drynariae against RANKL-induced osteoclastogenesis in vitro

Chi Fung Cheng, Jeff Chien-Fu Lin, Fuu Jen Tsai, Chao Jung Chen, Jian Shiun Chiou, Chen Hsing Chou, Te Mao Li, Ting Hsu Lin, Chiu Chu Liao, Shao Mei Huang, Ju Pi Li, Jung Chun Lin, Chih Chien Lin, Bo Ban, Wen Miin Liang, Ying Ju Lin

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Ethnopharmacological relevance: Osteoporosis is one of the most common bone diseases; it is characterized by bone loss and is a risk factor for hip fracture. Chinese herbal medicines (CHMs) and their related natural compounds have been used for treating many diseases, including bone diseases, since ancient times in China and are regarded as a cost-effective complementary therapy. Aim of the study: The goal of this study was to investigate the osteoprotective mechanisms of these three Chinese herbs and their related natural compounds. The effects of CHMs and related natural compounds on RANKL-induced osteoclastogenesis in vitro were investigated. Materials and methods: A network pharmacology method was applied to study CHM-related natural compounds and their osteoporosis targets. In addition, their effect on RANKL-induced osteoclastogenesis in RAW264.7 cells was also investigated in vitro. Results: Radix dipsaci, Eucommiae cortex, and Rhizoma drynariae exhibited protective effects against mortality in hip fracture patients. Furthermore, these three herbs inhibited RANKL-induced TRAP activities and reduced the expression of bone resorption-related genes in RAW264.7 cells. Network analysis of natural compound (ingredient)-target interactions identified 11 natural compounds. Signal pathway analyses suggested that these compounds may target cytokine-cytokine receptor interactions, including RANKL-induced osteoclastogenesis. Five novel natural compounds exhibited reduced RANKL-induced TRAP activities and bone resorption-related gene expression. Conclusion: The clinically used CHMs, Radix dipsaci, Eucommiae cortex, and Rhizoma drynariae, and natural compounds obtained from them may suppress RANKL-induced osteoclastogenesis in vitro.

Original languageEnglish
Article number112074
JournalJournal of Ethnopharmacology
Volume244
DOIs
Publication statusPublished - Nov 15 2019

Fingerprint

Polypodiaceae
Herbal Medicine
Osteogenesis
Bone Diseases
Hip Fractures
Bone Resorption
Osteoporosis
Cytokine Receptors
Complementary Therapies
Signal Transduction
China
Pharmacology
Cytokines
Gene Expression
Costs and Cost Analysis
Bone and Bones
Mortality
In Vitro Techniques
Genes

Keywords

  • Chinese herbal medicine
  • Hip fracture
  • Natural compound
  • Osteoclastogenesis
  • Osteoporosis

ASJC Scopus subject areas

  • Pharmacology
  • Drug Discovery

Cite this

Protective effects and network analysis of natural compounds obtained from Radix dipsaci, Eucommiae cortex, and Rhizoma drynariae against RANKL-induced osteoclastogenesis in vitro. / Cheng, Chi Fung; Chien-Fu Lin, Jeff; Tsai, Fuu Jen; Chen, Chao Jung; Chiou, Jian Shiun; Chou, Chen Hsing; Li, Te Mao; Lin, Ting Hsu; Liao, Chiu Chu; Huang, Shao Mei; Li, Ju Pi; Lin, Jung Chun; Lin, Chih Chien; Ban, Bo; Liang, Wen Miin; Lin, Ying Ju.

In: Journal of Ethnopharmacology, Vol. 244, 112074, 15.11.2019.

Research output: Contribution to journalArticle

Cheng, Chi Fung ; Chien-Fu Lin, Jeff ; Tsai, Fuu Jen ; Chen, Chao Jung ; Chiou, Jian Shiun ; Chou, Chen Hsing ; Li, Te Mao ; Lin, Ting Hsu ; Liao, Chiu Chu ; Huang, Shao Mei ; Li, Ju Pi ; Lin, Jung Chun ; Lin, Chih Chien ; Ban, Bo ; Liang, Wen Miin ; Lin, Ying Ju. / Protective effects and network analysis of natural compounds obtained from Radix dipsaci, Eucommiae cortex, and Rhizoma drynariae against RANKL-induced osteoclastogenesis in vitro. In: Journal of Ethnopharmacology. 2019 ; Vol. 244.
@article{8958106ed62440daac4ae963034e6929,
title = "Protective effects and network analysis of natural compounds obtained from Radix dipsaci, Eucommiae cortex, and Rhizoma drynariae against RANKL-induced osteoclastogenesis in vitro",
abstract = "Ethnopharmacological relevance: Osteoporosis is one of the most common bone diseases; it is characterized by bone loss and is a risk factor for hip fracture. Chinese herbal medicines (CHMs) and their related natural compounds have been used for treating many diseases, including bone diseases, since ancient times in China and are regarded as a cost-effective complementary therapy. Aim of the study: The goal of this study was to investigate the osteoprotective mechanisms of these three Chinese herbs and their related natural compounds. The effects of CHMs and related natural compounds on RANKL-induced osteoclastogenesis in vitro were investigated. Materials and methods: A network pharmacology method was applied to study CHM-related natural compounds and their osteoporosis targets. In addition, their effect on RANKL-induced osteoclastogenesis in RAW264.7 cells was also investigated in vitro. Results: Radix dipsaci, Eucommiae cortex, and Rhizoma drynariae exhibited protective effects against mortality in hip fracture patients. Furthermore, these three herbs inhibited RANKL-induced TRAP activities and reduced the expression of bone resorption-related genes in RAW264.7 cells. Network analysis of natural compound (ingredient)-target interactions identified 11 natural compounds. Signal pathway analyses suggested that these compounds may target cytokine-cytokine receptor interactions, including RANKL-induced osteoclastogenesis. Five novel natural compounds exhibited reduced RANKL-induced TRAP activities and bone resorption-related gene expression. Conclusion: The clinically used CHMs, Radix dipsaci, Eucommiae cortex, and Rhizoma drynariae, and natural compounds obtained from them may suppress RANKL-induced osteoclastogenesis in vitro.",
keywords = "Chinese herbal medicine, Hip fracture, Natural compound, Osteoclastogenesis, Osteoporosis",
author = "Cheng, {Chi Fung} and {Chien-Fu Lin}, Jeff and Tsai, {Fuu Jen} and Chen, {Chao Jung} and Chiou, {Jian Shiun} and Chou, {Chen Hsing} and Li, {Te Mao} and Lin, {Ting Hsu} and Liao, {Chiu Chu} and Huang, {Shao Mei} and Li, {Ju Pi} and Lin, {Jung Chun} and Lin, {Chih Chien} and Bo Ban and Liang, {Wen Miin} and Lin, {Ying Ju}",
year = "2019",
month = "11",
day = "15",
doi = "10.1016/j.jep.2019.112074",
language = "English",
volume = "244",
journal = "Journal of Ethnopharmacology",
issn = "0378-8741",
publisher = "Elsevier Ireland Ltd",

}

TY - JOUR

T1 - Protective effects and network analysis of natural compounds obtained from Radix dipsaci, Eucommiae cortex, and Rhizoma drynariae against RANKL-induced osteoclastogenesis in vitro

AU - Cheng, Chi Fung

AU - Chien-Fu Lin, Jeff

AU - Tsai, Fuu Jen

AU - Chen, Chao Jung

AU - Chiou, Jian Shiun

AU - Chou, Chen Hsing

AU - Li, Te Mao

AU - Lin, Ting Hsu

AU - Liao, Chiu Chu

AU - Huang, Shao Mei

AU - Li, Ju Pi

AU - Lin, Jung Chun

AU - Lin, Chih Chien

AU - Ban, Bo

AU - Liang, Wen Miin

AU - Lin, Ying Ju

PY - 2019/11/15

Y1 - 2019/11/15

N2 - Ethnopharmacological relevance: Osteoporosis is one of the most common bone diseases; it is characterized by bone loss and is a risk factor for hip fracture. Chinese herbal medicines (CHMs) and their related natural compounds have been used for treating many diseases, including bone diseases, since ancient times in China and are regarded as a cost-effective complementary therapy. Aim of the study: The goal of this study was to investigate the osteoprotective mechanisms of these three Chinese herbs and their related natural compounds. The effects of CHMs and related natural compounds on RANKL-induced osteoclastogenesis in vitro were investigated. Materials and methods: A network pharmacology method was applied to study CHM-related natural compounds and their osteoporosis targets. In addition, their effect on RANKL-induced osteoclastogenesis in RAW264.7 cells was also investigated in vitro. Results: Radix dipsaci, Eucommiae cortex, and Rhizoma drynariae exhibited protective effects against mortality in hip fracture patients. Furthermore, these three herbs inhibited RANKL-induced TRAP activities and reduced the expression of bone resorption-related genes in RAW264.7 cells. Network analysis of natural compound (ingredient)-target interactions identified 11 natural compounds. Signal pathway analyses suggested that these compounds may target cytokine-cytokine receptor interactions, including RANKL-induced osteoclastogenesis. Five novel natural compounds exhibited reduced RANKL-induced TRAP activities and bone resorption-related gene expression. Conclusion: The clinically used CHMs, Radix dipsaci, Eucommiae cortex, and Rhizoma drynariae, and natural compounds obtained from them may suppress RANKL-induced osteoclastogenesis in vitro.

AB - Ethnopharmacological relevance: Osteoporosis is one of the most common bone diseases; it is characterized by bone loss and is a risk factor for hip fracture. Chinese herbal medicines (CHMs) and their related natural compounds have been used for treating many diseases, including bone diseases, since ancient times in China and are regarded as a cost-effective complementary therapy. Aim of the study: The goal of this study was to investigate the osteoprotective mechanisms of these three Chinese herbs and their related natural compounds. The effects of CHMs and related natural compounds on RANKL-induced osteoclastogenesis in vitro were investigated. Materials and methods: A network pharmacology method was applied to study CHM-related natural compounds and their osteoporosis targets. In addition, their effect on RANKL-induced osteoclastogenesis in RAW264.7 cells was also investigated in vitro. Results: Radix dipsaci, Eucommiae cortex, and Rhizoma drynariae exhibited protective effects against mortality in hip fracture patients. Furthermore, these three herbs inhibited RANKL-induced TRAP activities and reduced the expression of bone resorption-related genes in RAW264.7 cells. Network analysis of natural compound (ingredient)-target interactions identified 11 natural compounds. Signal pathway analyses suggested that these compounds may target cytokine-cytokine receptor interactions, including RANKL-induced osteoclastogenesis. Five novel natural compounds exhibited reduced RANKL-induced TRAP activities and bone resorption-related gene expression. Conclusion: The clinically used CHMs, Radix dipsaci, Eucommiae cortex, and Rhizoma drynariae, and natural compounds obtained from them may suppress RANKL-induced osteoclastogenesis in vitro.

KW - Chinese herbal medicine

KW - Hip fracture

KW - Natural compound

KW - Osteoclastogenesis

KW - Osteoporosis

UR - http://www.scopus.com/inward/record.url?scp=85070103941&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85070103941&partnerID=8YFLogxK

U2 - 10.1016/j.jep.2019.112074

DO - 10.1016/j.jep.2019.112074

M3 - Article

C2 - 31291608

AN - SCOPUS:85070103941

VL - 244

JO - Journal of Ethnopharmacology

JF - Journal of Ethnopharmacology

SN - 0378-8741

M1 - 112074

ER -