Protective Effect of Melatonin against Oxidative Stress-Induced Apoptosis and Enhanced Autophagy in Human Retinal Pigment Epithelium Cells

Chih Chao Chang, Tien Yi Huang, Hsin Yuan Chen, Tsui Chin Huang, Li Chun Lin, Yen Jui Chang, Shih Min Hsia

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Age-related macular degeneration (AMD) affects the retinal macula and results in loss of vision, and AMD is the primary cause of blindness and severe visual impairment among elderly people worldwide. AMD is characterized by the accumulation of drusen in the Bruch's membrane and dysfunction of retinal pigment epithelial (RPE) cells and photoreceptors. The pathogenesis of AMD remains unclear, and no effective treatment exists. Accumulating evidence indicates that oxidative stress plays a critical role in RPE cell degeneration and AMD. Melatonin is an antioxidant that scavenges free radicals, and it has anti-inflammatory, antitumor, and antiangiogenic effects. This study investigated the antioxidative, antiapoptotic, and autophagic effects of melatonin on oxidative damage to RPE cells. We used hydrogen peroxide (H2O2) to stimulate reactive oxygen species production to cause cell apoptosis in ARPE-19 cell lines. Our findings revealed that treatment with melatonin significantly inhibited H2O2-induced RPE cell damage, decreased the apoptotic rate, increased the mitochondrial membrane potential, and increased the autophagy effect. Furthermore, melatonin reduced the Bax/Bcl-2 ratio and the expression levels of the apoptosis-Associated proteins cytochrome c and caspase 7. Additionally, melatonin upregulated the expression of the autophagy-related proteins LC3-II and Beclin-1 and downregulated the expression of p62. Thus, melatonin's effects on autophagy and apoptosis can protect against H2O2-induced oxidative damage in human RPE cells. Melatonin may have multiple protective effects on human RPE cells against H2O2-induced oxidative damage.

Original languageEnglish
Article number9015765
JournalOxidative Medicine and Cellular Longevity
Volume2018
DOIs
Publication statusPublished - Jan 1 2018

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Oxidative stress
Retinal Pigments
Retinal Pigment Epithelium
Autophagy
Melatonin
Oxidative Stress
Macular Degeneration
Apoptosis
Epithelial Cells
Membranes
Caspase 7
Mitochondrial Membrane Potential
Vision Disorders
Blindness
Cytochromes c
Hydrogen Peroxide
Free Radicals
Reactive Oxygen Species
Proteins
Anti-Inflammatory Agents

ASJC Scopus subject areas

  • Biochemistry
  • Ageing
  • Cell Biology

Cite this

Protective Effect of Melatonin against Oxidative Stress-Induced Apoptosis and Enhanced Autophagy in Human Retinal Pigment Epithelium Cells. / Chang, Chih Chao; Huang, Tien Yi; Chen, Hsin Yuan; Huang, Tsui Chin; Lin, Li Chun; Chang, Yen Jui; Hsia, Shih Min.

In: Oxidative Medicine and Cellular Longevity, Vol. 2018, 9015765, 01.01.2018.

Research output: Contribution to journalArticle

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