Protective effect of a gonadotropin-releasing hormone analogue on chemotherapeutic agent-induced ovarian gonadotoxicity: A mouse model

Shun Jen Tan, Yu Chi Yeh, Wei Jen Shang, Gwo Jang Wu, Jah Yao Liu, Chi Huang Chen

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Objective: To demonstrate the protective effect of triptorelin, a GnRH analogue, on chemotherapy-induced ovarian gonadotoxicity. Study design: Twenty-four sexually mature, virgin, female FVB/NJNarl mice were divided into four groups: busulfan (B); low-dose triptorelin plus busulfan (T L + B); high-dose triptorelin plus busulfan (T H + B); and control. Mice in the T L + B and T H + B groups were injected with 3.8 and 38 mg/kg of triptorelin subcutaneously, respectively. Four weeks later, mice in the B, T L + B, and T H + B groups were injected with busulfan intraperitoneally at a dose of 36 mg/kg. Histologic examinations were performed 4 weeks later. Results: Obvious destruction of ovarian structure and significant depletion of primordial, primary, and secondary follicles were demonstrated in the B group compared with the control group, affirming the gonadotoxicity of busulfan. In the T L + B group, a greater number of larger primordial and primary follicles were enumerated compared with the B group; however, statistical significance was not achieved. In the T H + B group, the number of primordial and primary follicles was significantly greater than in the B group, and the ovarian tissue in the T H + B group was spared, demonstrating the effect of triptorelin pre-treatment on ovarian protection. Conclusion: Our results have demonstrated a dose-dependent protective effect against gonadotoxic chemotherapy of a GnRH analogue on ovarian reserve, thus suggesting a novel application of GnRH analogues in fertility preservation. Crown

Original languageEnglish
Pages (from-to)182-185
Number of pages4
JournalEuropean Journal of Obstetrics Gynecology and Reproductive Biology
Volume149
Issue number2
DOIs
Publication statusPublished - Apr 2010
Externally publishedYes

Fingerprint

Triptorelin Pamoate
Busulfan
Gonadotropin-Releasing Hormone
Fertility Preservation
Drug Therapy
Crowns
Control Groups

Keywords

  • Fertility preservation
  • GnRH analogue
  • Gonadotoxicity
  • Ovarian protection
  • Primordial follicles

ASJC Scopus subject areas

  • Obstetrics and Gynaecology
  • Reproductive Medicine

Cite this

Protective effect of a gonadotropin-releasing hormone analogue on chemotherapeutic agent-induced ovarian gonadotoxicity : A mouse model. / Tan, Shun Jen; Yeh, Yu Chi; Shang, Wei Jen; Wu, Gwo Jang; Liu, Jah Yao; Chen, Chi Huang.

In: European Journal of Obstetrics Gynecology and Reproductive Biology, Vol. 149, No. 2, 04.2010, p. 182-185.

Research output: Contribution to journalArticle

@article{60494f3654a446e6b1f433ce48696f00,
title = "Protective effect of a gonadotropin-releasing hormone analogue on chemotherapeutic agent-induced ovarian gonadotoxicity: A mouse model",
abstract = "Objective: To demonstrate the protective effect of triptorelin, a GnRH analogue, on chemotherapy-induced ovarian gonadotoxicity. Study design: Twenty-four sexually mature, virgin, female FVB/NJNarl mice were divided into four groups: busulfan (B); low-dose triptorelin plus busulfan (T L + B); high-dose triptorelin plus busulfan (T H + B); and control. Mice in the T L + B and T H + B groups were injected with 3.8 and 38 mg/kg of triptorelin subcutaneously, respectively. Four weeks later, mice in the B, T L + B, and T H + B groups were injected with busulfan intraperitoneally at a dose of 36 mg/kg. Histologic examinations were performed 4 weeks later. Results: Obvious destruction of ovarian structure and significant depletion of primordial, primary, and secondary follicles were demonstrated in the B group compared with the control group, affirming the gonadotoxicity of busulfan. In the T L + B group, a greater number of larger primordial and primary follicles were enumerated compared with the B group; however, statistical significance was not achieved. In the T H + B group, the number of primordial and primary follicles was significantly greater than in the B group, and the ovarian tissue in the T H + B group was spared, demonstrating the effect of triptorelin pre-treatment on ovarian protection. Conclusion: Our results have demonstrated a dose-dependent protective effect against gonadotoxic chemotherapy of a GnRH analogue on ovarian reserve, thus suggesting a novel application of GnRH analogues in fertility preservation. Crown",
keywords = "Fertility preservation, GnRH analogue, Gonadotoxicity, Ovarian protection, Primordial follicles",
author = "Tan, {Shun Jen} and Yeh, {Yu Chi} and Shang, {Wei Jen} and Wu, {Gwo Jang} and Liu, {Jah Yao} and Chen, {Chi Huang}",
year = "2010",
month = "4",
doi = "10.1016/j.ejogrb.2009.12.028",
language = "English",
volume = "149",
pages = "182--185",
journal = "European Journal of Obstetrics and Gynecology and Reproductive Biology",
issn = "0028-2243",
publisher = "Elsevier Ireland Ltd",
number = "2",

}

TY - JOUR

T1 - Protective effect of a gonadotropin-releasing hormone analogue on chemotherapeutic agent-induced ovarian gonadotoxicity

T2 - A mouse model

AU - Tan, Shun Jen

AU - Yeh, Yu Chi

AU - Shang, Wei Jen

AU - Wu, Gwo Jang

AU - Liu, Jah Yao

AU - Chen, Chi Huang

PY - 2010/4

Y1 - 2010/4

N2 - Objective: To demonstrate the protective effect of triptorelin, a GnRH analogue, on chemotherapy-induced ovarian gonadotoxicity. Study design: Twenty-four sexually mature, virgin, female FVB/NJNarl mice were divided into four groups: busulfan (B); low-dose triptorelin plus busulfan (T L + B); high-dose triptorelin plus busulfan (T H + B); and control. Mice in the T L + B and T H + B groups were injected with 3.8 and 38 mg/kg of triptorelin subcutaneously, respectively. Four weeks later, mice in the B, T L + B, and T H + B groups were injected with busulfan intraperitoneally at a dose of 36 mg/kg. Histologic examinations were performed 4 weeks later. Results: Obvious destruction of ovarian structure and significant depletion of primordial, primary, and secondary follicles were demonstrated in the B group compared with the control group, affirming the gonadotoxicity of busulfan. In the T L + B group, a greater number of larger primordial and primary follicles were enumerated compared with the B group; however, statistical significance was not achieved. In the T H + B group, the number of primordial and primary follicles was significantly greater than in the B group, and the ovarian tissue in the T H + B group was spared, demonstrating the effect of triptorelin pre-treatment on ovarian protection. Conclusion: Our results have demonstrated a dose-dependent protective effect against gonadotoxic chemotherapy of a GnRH analogue on ovarian reserve, thus suggesting a novel application of GnRH analogues in fertility preservation. Crown

AB - Objective: To demonstrate the protective effect of triptorelin, a GnRH analogue, on chemotherapy-induced ovarian gonadotoxicity. Study design: Twenty-four sexually mature, virgin, female FVB/NJNarl mice were divided into four groups: busulfan (B); low-dose triptorelin plus busulfan (T L + B); high-dose triptorelin plus busulfan (T H + B); and control. Mice in the T L + B and T H + B groups were injected with 3.8 and 38 mg/kg of triptorelin subcutaneously, respectively. Four weeks later, mice in the B, T L + B, and T H + B groups were injected with busulfan intraperitoneally at a dose of 36 mg/kg. Histologic examinations were performed 4 weeks later. Results: Obvious destruction of ovarian structure and significant depletion of primordial, primary, and secondary follicles were demonstrated in the B group compared with the control group, affirming the gonadotoxicity of busulfan. In the T L + B group, a greater number of larger primordial and primary follicles were enumerated compared with the B group; however, statistical significance was not achieved. In the T H + B group, the number of primordial and primary follicles was significantly greater than in the B group, and the ovarian tissue in the T H + B group was spared, demonstrating the effect of triptorelin pre-treatment on ovarian protection. Conclusion: Our results have demonstrated a dose-dependent protective effect against gonadotoxic chemotherapy of a GnRH analogue on ovarian reserve, thus suggesting a novel application of GnRH analogues in fertility preservation. Crown

KW - Fertility preservation

KW - GnRH analogue

KW - Gonadotoxicity

KW - Ovarian protection

KW - Primordial follicles

UR - http://www.scopus.com/inward/record.url?scp=77549087226&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77549087226&partnerID=8YFLogxK

U2 - 10.1016/j.ejogrb.2009.12.028

DO - 10.1016/j.ejogrb.2009.12.028

M3 - Article

C2 - 20074846

AN - SCOPUS:77549087226

VL - 149

SP - 182

EP - 185

JO - European Journal of Obstetrics and Gynecology and Reproductive Biology

JF - European Journal of Obstetrics and Gynecology and Reproductive Biology

SN - 0028-2243

IS - 2

ER -