Protection of small bowel from ischemia and re-perfusion injury using luminal fetal bovine serum perfusion

Wen Tsung Hung, Chia Lang Fang, Chien Ts Chu, Jyy Jih Tsai-Wu

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

A fetal or newborn (rat) organ (eg intestine, kidney, skin or lung) transplanted into a syngenic adult host without any vascular anastomosis is able to survive. We hypothesize that fetal tissue possesses an innate ability to prevent ischemia reperfusion injury, such "factors" being developed during pregnancy. We created an experimental model to induce total vascular occlusion in a 15-cm long rat ileal segment. Using an osmotic minipump implanted at the subcutaneous space of the abdominal wall of a test rat, fetal bovine serum (FBS) luminal perfusion was conducted. Three days prior to the three-hour total vascular occlusion had been induced, luminal perfusion using FBS commenced for the test animals. On day zero, day three and day six following the release of vascular occlusion, the test rats were sacrificed and tissue samples were obtained for morphological study and intestinal DNA and protein content measurement. We used a scale where the occlusion-induced morphological changes to the intestinal mucosa were graded into one of six different levels of mucosal damage. The greater the numerical rating, the more substantial morphological changes to the gut wall. On data zero,the mean grades of the morphologically apparent intestinal deterioration were both rated at a level of five for the control and the experimental group. The mean values were 3.1 and 2.1 on day three, and 2.6 and 1.5 on day six, for the control and experimental control group was 2.26 ± 0.52 (μg/mg) on day zero, 3.46 ± 0.68 (μg/mg) on day 3 and 3.50 ± 0.72 (μg/mg) on day 6. DNA content of the experimental group on day zero was 2.79 ± 0.78 (μg/mg), 4.54 ± 0.76 (μg/mg) on day 3 and 4.49 ± 0.49 (μg/mg) on day 6. Protein content of the control group on day zero was 31.52 ± 2.96 μg/mg), 54.85 ± 4.16 (μg/mg) on day 3 and 56.12 ± 3.93 (μg/mg) on day 6. Protein content of the experimental group was 57.93 ± 5.48 (μg/mg) on day zero, 74.69 ± 9.50 (μg/mg) on day 3 and 71.72 ± 7.02 (μg/mg) on day 6. From the data described above, luminal perfusion using FBS could, under certain circumstances, protect the small bowel from ischemia and reperfusion injury and also increase intestinal mass.

Original languageEnglish
Pages (from-to)235-240
Number of pages6
JournalFormosan Journal of Surgery
Volume35
Issue number5
Publication statusPublished - 2002
Externally publishedYes

Fingerprint

Blood Vessels
Ischemia
Perfusion
Wounds and Injuries
Reperfusion Injury
Serum
Control Groups
Proteins
DNA
Abdominal Wall
Intestinal Mucosa
Intestines
Fetus
Theoretical Models
Kidney
Pregnancy
Lung
Skin

Keywords

  • Fetal bovine serum
  • Ischemia-reperfusion injury

ASJC Scopus subject areas

  • Surgery

Cite this

Protection of small bowel from ischemia and re-perfusion injury using luminal fetal bovine serum perfusion. / Hung, Wen Tsung; Fang, Chia Lang; Chu, Chien Ts; Tsai-Wu, Jyy Jih.

In: Formosan Journal of Surgery, Vol. 35, No. 5, 2002, p. 235-240.

Research output: Contribution to journalArticle

@article{1b918fcc9e7445b8a2116f20dc0d8f3d,
title = "Protection of small bowel from ischemia and re-perfusion injury using luminal fetal bovine serum perfusion",
abstract = "A fetal or newborn (rat) organ (eg intestine, kidney, skin or lung) transplanted into a syngenic adult host without any vascular anastomosis is able to survive. We hypothesize that fetal tissue possesses an innate ability to prevent ischemia reperfusion injury, such {"}factors{"} being developed during pregnancy. We created an experimental model to induce total vascular occlusion in a 15-cm long rat ileal segment. Using an osmotic minipump implanted at the subcutaneous space of the abdominal wall of a test rat, fetal bovine serum (FBS) luminal perfusion was conducted. Three days prior to the three-hour total vascular occlusion had been induced, luminal perfusion using FBS commenced for the test animals. On day zero, day three and day six following the release of vascular occlusion, the test rats were sacrificed and tissue samples were obtained for morphological study and intestinal DNA and protein content measurement. We used a scale where the occlusion-induced morphological changes to the intestinal mucosa were graded into one of six different levels of mucosal damage. The greater the numerical rating, the more substantial morphological changes to the gut wall. On data zero,the mean grades of the morphologically apparent intestinal deterioration were both rated at a level of five for the control and the experimental group. The mean values were 3.1 and 2.1 on day three, and 2.6 and 1.5 on day six, for the control and experimental control group was 2.26 ± 0.52 (μg/mg) on day zero, 3.46 ± 0.68 (μg/mg) on day 3 and 3.50 ± 0.72 (μg/mg) on day 6. DNA content of the experimental group on day zero was 2.79 ± 0.78 (μg/mg), 4.54 ± 0.76 (μg/mg) on day 3 and 4.49 ± 0.49 (μg/mg) on day 6. Protein content of the control group on day zero was 31.52 ± 2.96 μg/mg), 54.85 ± 4.16 (μg/mg) on day 3 and 56.12 ± 3.93 (μg/mg) on day 6. Protein content of the experimental group was 57.93 ± 5.48 (μg/mg) on day zero, 74.69 ± 9.50 (μg/mg) on day 3 and 71.72 ± 7.02 (μg/mg) on day 6. From the data described above, luminal perfusion using FBS could, under certain circumstances, protect the small bowel from ischemia and reperfusion injury and also increase intestinal mass.",
keywords = "Fetal bovine serum, Ischemia-reperfusion injury",
author = "Hung, {Wen Tsung} and Fang, {Chia Lang} and Chu, {Chien Ts} and Tsai-Wu, {Jyy Jih}",
year = "2002",
language = "English",
volume = "35",
pages = "235--240",
journal = "Formosan Journal of Surgery",
issn = "1011-6788",
publisher = "臺灣外科醫學會",
number = "5",

}

TY - JOUR

T1 - Protection of small bowel from ischemia and re-perfusion injury using luminal fetal bovine serum perfusion

AU - Hung, Wen Tsung

AU - Fang, Chia Lang

AU - Chu, Chien Ts

AU - Tsai-Wu, Jyy Jih

PY - 2002

Y1 - 2002

N2 - A fetal or newborn (rat) organ (eg intestine, kidney, skin or lung) transplanted into a syngenic adult host without any vascular anastomosis is able to survive. We hypothesize that fetal tissue possesses an innate ability to prevent ischemia reperfusion injury, such "factors" being developed during pregnancy. We created an experimental model to induce total vascular occlusion in a 15-cm long rat ileal segment. Using an osmotic minipump implanted at the subcutaneous space of the abdominal wall of a test rat, fetal bovine serum (FBS) luminal perfusion was conducted. Three days prior to the three-hour total vascular occlusion had been induced, luminal perfusion using FBS commenced for the test animals. On day zero, day three and day six following the release of vascular occlusion, the test rats were sacrificed and tissue samples were obtained for morphological study and intestinal DNA and protein content measurement. We used a scale where the occlusion-induced morphological changes to the intestinal mucosa were graded into one of six different levels of mucosal damage. The greater the numerical rating, the more substantial morphological changes to the gut wall. On data zero,the mean grades of the morphologically apparent intestinal deterioration were both rated at a level of five for the control and the experimental group. The mean values were 3.1 and 2.1 on day three, and 2.6 and 1.5 on day six, for the control and experimental control group was 2.26 ± 0.52 (μg/mg) on day zero, 3.46 ± 0.68 (μg/mg) on day 3 and 3.50 ± 0.72 (μg/mg) on day 6. DNA content of the experimental group on day zero was 2.79 ± 0.78 (μg/mg), 4.54 ± 0.76 (μg/mg) on day 3 and 4.49 ± 0.49 (μg/mg) on day 6. Protein content of the control group on day zero was 31.52 ± 2.96 μg/mg), 54.85 ± 4.16 (μg/mg) on day 3 and 56.12 ± 3.93 (μg/mg) on day 6. Protein content of the experimental group was 57.93 ± 5.48 (μg/mg) on day zero, 74.69 ± 9.50 (μg/mg) on day 3 and 71.72 ± 7.02 (μg/mg) on day 6. From the data described above, luminal perfusion using FBS could, under certain circumstances, protect the small bowel from ischemia and reperfusion injury and also increase intestinal mass.

AB - A fetal or newborn (rat) organ (eg intestine, kidney, skin or lung) transplanted into a syngenic adult host without any vascular anastomosis is able to survive. We hypothesize that fetal tissue possesses an innate ability to prevent ischemia reperfusion injury, such "factors" being developed during pregnancy. We created an experimental model to induce total vascular occlusion in a 15-cm long rat ileal segment. Using an osmotic minipump implanted at the subcutaneous space of the abdominal wall of a test rat, fetal bovine serum (FBS) luminal perfusion was conducted. Three days prior to the three-hour total vascular occlusion had been induced, luminal perfusion using FBS commenced for the test animals. On day zero, day three and day six following the release of vascular occlusion, the test rats were sacrificed and tissue samples were obtained for morphological study and intestinal DNA and protein content measurement. We used a scale where the occlusion-induced morphological changes to the intestinal mucosa were graded into one of six different levels of mucosal damage. The greater the numerical rating, the more substantial morphological changes to the gut wall. On data zero,the mean grades of the morphologically apparent intestinal deterioration were both rated at a level of five for the control and the experimental group. The mean values were 3.1 and 2.1 on day three, and 2.6 and 1.5 on day six, for the control and experimental control group was 2.26 ± 0.52 (μg/mg) on day zero, 3.46 ± 0.68 (μg/mg) on day 3 and 3.50 ± 0.72 (μg/mg) on day 6. DNA content of the experimental group on day zero was 2.79 ± 0.78 (μg/mg), 4.54 ± 0.76 (μg/mg) on day 3 and 4.49 ± 0.49 (μg/mg) on day 6. Protein content of the control group on day zero was 31.52 ± 2.96 μg/mg), 54.85 ± 4.16 (μg/mg) on day 3 and 56.12 ± 3.93 (μg/mg) on day 6. Protein content of the experimental group was 57.93 ± 5.48 (μg/mg) on day zero, 74.69 ± 9.50 (μg/mg) on day 3 and 71.72 ± 7.02 (μg/mg) on day 6. From the data described above, luminal perfusion using FBS could, under certain circumstances, protect the small bowel from ischemia and reperfusion injury and also increase intestinal mass.

KW - Fetal bovine serum

KW - Ischemia-reperfusion injury

UR - http://www.scopus.com/inward/record.url?scp=0036757014&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036757014&partnerID=8YFLogxK

M3 - Article

VL - 35

SP - 235

EP - 240

JO - Formosan Journal of Surgery

JF - Formosan Journal of Surgery

SN - 1011-6788

IS - 5

ER -