Protection of neonatal mice from lethal enterovirus 71 infection by maternal immunization with attenuated Salmonella enterica serovar Typhimurium expressing VP1 of enterovirus 71

Cheng Hsun Chiu, Chishih Chu, Chao Che He, Tzou Yien Lin

Research output: Contribution to journalArticle

68 Citations (Scopus)

Abstract

This study describes the potential use of attenuated Salmonella enterica serovar Typhimurium strains to express and deliver VP1 of enterovirus 71 (EV71) as a vaccination strategy to prevent EV71 infection in mice. When orally administered to BALB/c mice, both attenuated carrier strains, CNP101 and SL7207, were able to efficiently invade livers and spleens, while only the virulence plasmid-carrying strain SL7207 persisted for more than 30 days in these organs. A recombinant in vivo-regulated promoter expression plasmid expressing VP1 antigen of EV71 was constructed. The expression of the VP1, directed by the pagC promoter, in attenuated Salmonella was confirmed by Western blot hybridization. Both humoral and cellular immune responses were elicited in mice by oral immunization with such Salmonella-based VP1 vaccines. We evaluated the protective efficacy of the vaccines in mice using a maternal immunization protocol. With a lethal challenge, ICR newborn mice born to dams immunized with Salmonella-based VP1 vaccine showed a 50-60% survival; in contrast, none of the mice in the control group survived the challenge. Our data indicated that Salmonella-based VP1 subunit vaccines are a promising vaccine strategy in the prevention of EV71 infection.

Original languageEnglish
Pages (from-to)1671-1678
Number of pages8
JournalMicrobes and Infection
Volume8
Issue number7
DOIs
Publication statusPublished - Jun 2006
Externally publishedYes

Fingerprint

Enterovirus Infections
Salmonella enterica
Enterovirus
Immunization
Salmonella
Mothers
Vaccines
Plasmids
Inbred ICR Mouse
Subunit Vaccines
Humoral Immunity
Cellular Immunity
Virulence
Vaccination
Spleen
Western Blotting
Serogroup
Antigens
Control Groups
Liver

Keywords

  • Attenuated Salmonella enterica serovar Typhimurium
  • Enterovirus 71
  • VP1 vaccine

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Infectious Diseases

Cite this

@article{9f9864003c044168a2f43c49fb77de14,
title = "Protection of neonatal mice from lethal enterovirus 71 infection by maternal immunization with attenuated Salmonella enterica serovar Typhimurium expressing VP1 of enterovirus 71",
abstract = "This study describes the potential use of attenuated Salmonella enterica serovar Typhimurium strains to express and deliver VP1 of enterovirus 71 (EV71) as a vaccination strategy to prevent EV71 infection in mice. When orally administered to BALB/c mice, both attenuated carrier strains, CNP101 and SL7207, were able to efficiently invade livers and spleens, while only the virulence plasmid-carrying strain SL7207 persisted for more than 30 days in these organs. A recombinant in vivo-regulated promoter expression plasmid expressing VP1 antigen of EV71 was constructed. The expression of the VP1, directed by the pagC promoter, in attenuated Salmonella was confirmed by Western blot hybridization. Both humoral and cellular immune responses were elicited in mice by oral immunization with such Salmonella-based VP1 vaccines. We evaluated the protective efficacy of the vaccines in mice using a maternal immunization protocol. With a lethal challenge, ICR newborn mice born to dams immunized with Salmonella-based VP1 vaccine showed a 50-60{\%} survival; in contrast, none of the mice in the control group survived the challenge. Our data indicated that Salmonella-based VP1 subunit vaccines are a promising vaccine strategy in the prevention of EV71 infection.",
keywords = "Attenuated Salmonella enterica serovar Typhimurium, Enterovirus 71, VP1 vaccine",
author = "Chiu, {Cheng Hsun} and Chishih Chu and He, {Chao Che} and Lin, {Tzou Yien}",
year = "2006",
month = "6",
doi = "10.1016/j.micinf.2006.01.021",
language = "English",
volume = "8",
pages = "1671--1678",
journal = "Microbes and Infection",
issn = "1286-4579",
publisher = "Elsevier Masson SAS",
number = "7",

}

TY - JOUR

T1 - Protection of neonatal mice from lethal enterovirus 71 infection by maternal immunization with attenuated Salmonella enterica serovar Typhimurium expressing VP1 of enterovirus 71

AU - Chiu, Cheng Hsun

AU - Chu, Chishih

AU - He, Chao Che

AU - Lin, Tzou Yien

PY - 2006/6

Y1 - 2006/6

N2 - This study describes the potential use of attenuated Salmonella enterica serovar Typhimurium strains to express and deliver VP1 of enterovirus 71 (EV71) as a vaccination strategy to prevent EV71 infection in mice. When orally administered to BALB/c mice, both attenuated carrier strains, CNP101 and SL7207, were able to efficiently invade livers and spleens, while only the virulence plasmid-carrying strain SL7207 persisted for more than 30 days in these organs. A recombinant in vivo-regulated promoter expression plasmid expressing VP1 antigen of EV71 was constructed. The expression of the VP1, directed by the pagC promoter, in attenuated Salmonella was confirmed by Western blot hybridization. Both humoral and cellular immune responses were elicited in mice by oral immunization with such Salmonella-based VP1 vaccines. We evaluated the protective efficacy of the vaccines in mice using a maternal immunization protocol. With a lethal challenge, ICR newborn mice born to dams immunized with Salmonella-based VP1 vaccine showed a 50-60% survival; in contrast, none of the mice in the control group survived the challenge. Our data indicated that Salmonella-based VP1 subunit vaccines are a promising vaccine strategy in the prevention of EV71 infection.

AB - This study describes the potential use of attenuated Salmonella enterica serovar Typhimurium strains to express and deliver VP1 of enterovirus 71 (EV71) as a vaccination strategy to prevent EV71 infection in mice. When orally administered to BALB/c mice, both attenuated carrier strains, CNP101 and SL7207, were able to efficiently invade livers and spleens, while only the virulence plasmid-carrying strain SL7207 persisted for more than 30 days in these organs. A recombinant in vivo-regulated promoter expression plasmid expressing VP1 antigen of EV71 was constructed. The expression of the VP1, directed by the pagC promoter, in attenuated Salmonella was confirmed by Western blot hybridization. Both humoral and cellular immune responses were elicited in mice by oral immunization with such Salmonella-based VP1 vaccines. We evaluated the protective efficacy of the vaccines in mice using a maternal immunization protocol. With a lethal challenge, ICR newborn mice born to dams immunized with Salmonella-based VP1 vaccine showed a 50-60% survival; in contrast, none of the mice in the control group survived the challenge. Our data indicated that Salmonella-based VP1 subunit vaccines are a promising vaccine strategy in the prevention of EV71 infection.

KW - Attenuated Salmonella enterica serovar Typhimurium

KW - Enterovirus 71

KW - VP1 vaccine

UR - http://www.scopus.com/inward/record.url?scp=33746436595&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33746436595&partnerID=8YFLogxK

U2 - 10.1016/j.micinf.2006.01.021

DO - 10.1016/j.micinf.2006.01.021

M3 - Article

C2 - 16815726

AN - SCOPUS:33746436595

VL - 8

SP - 1671

EP - 1678

JO - Microbes and Infection

JF - Microbes and Infection

SN - 1286-4579

IS - 7

ER -