Abstract
A new copper zinc complex (CZpbi), [(bipyridinyl)2Cu-μ-pbi- Zn(pbi)](ClO4)2, pbi = 2-(2-pyridyl)-benzimidazole, was synthesized to mimic copper zinc superoxide dismutase (CuZnSOD). CZpbi was then encapsulated onto a dye (fluorescein isothiocyanate, FITC) functionalized mesoporous silica nanoparticles (MSN) where MSN serves as a nanofactory platform for delivering the catalytic action of the center copper complex. We examined the inhibition of oxidative damage of HeLa cells in the presence of paraquat (PQ, 1,1′-dimethyl-4,4′-bipyridylium dichloride, an oxidative stressor). We further studied the cell viability and cell cycle process under the influence of paraquat stress in the presence of the SOD-mimic FITC-MSN-CZpbi material by flow cytometry and western blot test. We found the nano-sized FITC-MSN is an excellent nano-carrier for delivering the mimic SOD complexes to HeLa cells. Here the reactants are already inside the cell and the result is a promotion of cell function. We present the methodology of encapsulating bio-inspired SOD-mimic in FITC-MSN to achieve a better drug delivery vehicle and catalytic (detoxification) activity. The potency and the stability of SOD-mimic FITC-MSN-CZpbi may provide a new avenue in the future development of artificial antioxidant enzymes.
| Original language | English |
|---|---|
| Pages (from-to) | 6042-6052 |
| Number of pages | 11 |
| Journal | Journal of Materials Chemistry B |
| Volume | 1 |
| Issue number | 44 |
| DOIs | |
| Publication status | Published - Nov 28 2013 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Chemistry(all)
- Biomedical Engineering
- Medicine(all)
- Materials Science(all)
Cite this
Protection of HeLa cells against ROS stress by CuZnSOD mimic system. / Fang, Ya Cheng; Chen, Yi Ping; Chen, Chien Tsu; Lin, Tien Sung; Mou, Chung Yuan.
In: Journal of Materials Chemistry B, Vol. 1, No. 44, 28.11.2013, p. 6042-6052.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Protection of HeLa cells against ROS stress by CuZnSOD mimic system
AU - Fang, Ya Cheng
AU - Chen, Yi Ping
AU - Chen, Chien Tsu
AU - Lin, Tien Sung
AU - Mou, Chung Yuan
PY - 2013/11/28
Y1 - 2013/11/28
N2 - A new copper zinc complex (CZpbi), [(bipyridinyl)2Cu-μ-pbi- Zn(pbi)](ClO4)2, pbi = 2-(2-pyridyl)-benzimidazole, was synthesized to mimic copper zinc superoxide dismutase (CuZnSOD). CZpbi was then encapsulated onto a dye (fluorescein isothiocyanate, FITC) functionalized mesoporous silica nanoparticles (MSN) where MSN serves as a nanofactory platform for delivering the catalytic action of the center copper complex. We examined the inhibition of oxidative damage of HeLa cells in the presence of paraquat (PQ, 1,1′-dimethyl-4,4′-bipyridylium dichloride, an oxidative stressor). We further studied the cell viability and cell cycle process under the influence of paraquat stress in the presence of the SOD-mimic FITC-MSN-CZpbi material by flow cytometry and western blot test. We found the nano-sized FITC-MSN is an excellent nano-carrier for delivering the mimic SOD complexes to HeLa cells. Here the reactants are already inside the cell and the result is a promotion of cell function. We present the methodology of encapsulating bio-inspired SOD-mimic in FITC-MSN to achieve a better drug delivery vehicle and catalytic (detoxification) activity. The potency and the stability of SOD-mimic FITC-MSN-CZpbi may provide a new avenue in the future development of artificial antioxidant enzymes.
AB - A new copper zinc complex (CZpbi), [(bipyridinyl)2Cu-μ-pbi- Zn(pbi)](ClO4)2, pbi = 2-(2-pyridyl)-benzimidazole, was synthesized to mimic copper zinc superoxide dismutase (CuZnSOD). CZpbi was then encapsulated onto a dye (fluorescein isothiocyanate, FITC) functionalized mesoporous silica nanoparticles (MSN) where MSN serves as a nanofactory platform for delivering the catalytic action of the center copper complex. We examined the inhibition of oxidative damage of HeLa cells in the presence of paraquat (PQ, 1,1′-dimethyl-4,4′-bipyridylium dichloride, an oxidative stressor). We further studied the cell viability and cell cycle process under the influence of paraquat stress in the presence of the SOD-mimic FITC-MSN-CZpbi material by flow cytometry and western blot test. We found the nano-sized FITC-MSN is an excellent nano-carrier for delivering the mimic SOD complexes to HeLa cells. Here the reactants are already inside the cell and the result is a promotion of cell function. We present the methodology of encapsulating bio-inspired SOD-mimic in FITC-MSN to achieve a better drug delivery vehicle and catalytic (detoxification) activity. The potency and the stability of SOD-mimic FITC-MSN-CZpbi may provide a new avenue in the future development of artificial antioxidant enzymes.
UR - http://www.scopus.com/inward/record.url?scp=84886630108&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84886630108&partnerID=8YFLogxK
U2 - 10.1039/c3tb21052a
DO - 10.1039/c3tb21052a
M3 - Article
AN - SCOPUS:84886630108
VL - 1
SP - 6042
EP - 6052
JO - Journal of Materials Chemistry B
JF - Journal of Materials Chemistry B
SN - 2050-7518
IS - 44
ER -