Protection of HeLa cells against ROS stress by CuZnSOD mimic system

Ya Cheng Fang, Yi Ping Chen, Chien Tsu Chen, Tien Sung Lin, Chung Yuan Mou

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

A new copper zinc complex (CZpbi), [(bipyridinyl)2Cu-μ-pbi- Zn(pbi)](ClO4)2, pbi = 2-(2-pyridyl)-benzimidazole, was synthesized to mimic copper zinc superoxide dismutase (CuZnSOD). CZpbi was then encapsulated onto a dye (fluorescein isothiocyanate, FITC) functionalized mesoporous silica nanoparticles (MSN) where MSN serves as a nanofactory platform for delivering the catalytic action of the center copper complex. We examined the inhibition of oxidative damage of HeLa cells in the presence of paraquat (PQ, 1,1′-dimethyl-4,4′-bipyridylium dichloride, an oxidative stressor). We further studied the cell viability and cell cycle process under the influence of paraquat stress in the presence of the SOD-mimic FITC-MSN-CZpbi material by flow cytometry and western blot test. We found the nano-sized FITC-MSN is an excellent nano-carrier for delivering the mimic SOD complexes to HeLa cells. Here the reactants are already inside the cell and the result is a promotion of cell function. We present the methodology of encapsulating bio-inspired SOD-mimic in FITC-MSN to achieve a better drug delivery vehicle and catalytic (detoxification) activity. The potency and the stability of SOD-mimic FITC-MSN-CZpbi may provide a new avenue in the future development of artificial antioxidant enzymes.

Original languageEnglish
Pages (from-to)6042-6052
Number of pages11
JournalJournal of Materials Chemistry B
Volume1
Issue number44
DOIs
Publication statusPublished - Nov 28 2013
Externally publishedYes

Fingerprint

HeLa Cells
Silicon Dioxide
Nanoparticles
Fluorescein
Silica
Copper
Paraquat
Zinc
Cells
Detoxification
Flow cytometry
Antioxidants
Drug delivery
Superoxide Dismutase
Cell Survival
Cell Cycle
Flow Cytometry
Coloring Agents
Enzymes
Dyes

ASJC Scopus subject areas

  • Chemistry(all)
  • Biomedical Engineering
  • Medicine(all)
  • Materials Science(all)

Cite this

Protection of HeLa cells against ROS stress by CuZnSOD mimic system. / Fang, Ya Cheng; Chen, Yi Ping; Chen, Chien Tsu; Lin, Tien Sung; Mou, Chung Yuan.

In: Journal of Materials Chemistry B, Vol. 1, No. 44, 28.11.2013, p. 6042-6052.

Research output: Contribution to journalArticle

Fang, Ya Cheng ; Chen, Yi Ping ; Chen, Chien Tsu ; Lin, Tien Sung ; Mou, Chung Yuan. / Protection of HeLa cells against ROS stress by CuZnSOD mimic system. In: Journal of Materials Chemistry B. 2013 ; Vol. 1, No. 44. pp. 6042-6052.
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