Prostate specific antigen gene expression in androgen insensitive prostate carcinoma subculture cell line

Ke Hung Tsui, Tsui Hsia Feng, Li Chuan Chung, Chun Hsiang Chao, Phei Lang Chang, Horng Heng Juang

Research output: Contribution to journalArticlepeer-review

15 Citations (Scopus)

Abstract

A novel prostate cancer cell line (PC-J) was isolated from an androgen independent non-prostate specific antigen (non-PSA) producing carcinoma cell line. The homologous correlation between PC-J and PC-3 was determined by short tandem repeat analysis. The PSA promoter activity was detected by transient expression assay in the PC-J and LNCaP cells but not in androgen insensitive PC-3 cells. When the PC-J cells were cotransfected with androgen receptor, androgen receptor coactivators and PSA reporter vector cells, the reporter assays indicated that nuclear receptor coactivator 4 (NCOA4) but not androgen receptor activator 24 (ARA24) increased the sensitivity and maximum stimulation of dihydrotestosterone (DHT)-inducing PSA promoter activity. The RT-PCR assays revealed that the expression of several tumor markers, including interleukin-6, prostate stem cell antigen (PSCA), prostate epithelium-specific Ets transcription factor (PDEF) and matriptase, was lower in the PC-J cells than in the PC-3 cells. This cell model elucidated the regulation of PSA expression and enabled comparison of the gene profile at different stages of metastasis in prostatic carcinoma.

Original languageEnglish
Pages (from-to)1969-1976
Number of pages8
JournalAnticancer Research
Volume28
Issue number4 A
Publication statusPublished - Jul 2008
Externally publishedYes

Keywords

  • ARA24
  • IL-6
  • Matriptase
  • NCOA4
  • PEDF
  • Prostate
  • PSA
  • PSCA

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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