Propofol reduces nitric oxide biosynthesis in lipopolysaccharide-activated macrophages by downregulating the expression of inducible nitric oxide synthase

Ruei-Ming Chen, Gong-Jhe Wu, Yu-Ting Tai, Wei Zen Sun, Yi Ling Lin, Wen Chi Jean, Ta-Liang Chen

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

Nitric oxide is an active oxidant that contributes to the physiology and pathophysiology of macrophages. Propofol has been widely used in intravenous anesthesia. It possess antioxidant and immunomodulating effects. This study aimed to evaluate the effects of propofol on nitric oxide production in lipopolysaccharide-activated macrophages. Exposure of macrophages to propofol (25, 50 and 75 μM), to lipopolysaccharide (0.5, 1, 1.5 and 2 ng/ml) or to a combination ofpropofol and lipopolysaccharide did not affect cell viability. However, propofol at 100 μM led to significant cell death (P3 versus 92±6×103 arbitrary units, P2 versus 30±4×102 arbitrary units, P

Original languageEnglish
Pages (from-to)418-423
Number of pages6
JournalArchives of Toxicology
Volume77
Issue number7
DOIs
Publication statusPublished - Jul 1 2003

Fingerprint

Macrophages
Biosynthesis
Propofol
Nitric Oxide Synthase Type II
Lipopolysaccharides
Nitric Oxide
Down-Regulation
Intravenous Anesthesia
Physiology
Cell death
Oxidants
Cell Survival
Cell Death
Antioxidants
Cells

Keywords

  • Inducible nitric oxide synthase
  • Lipopoly saccharide
  • Macrophages
  • Nitric oxide
  • Propofol

ASJC Scopus subject areas

  • Toxicology
  • Health, Toxicology and Mutagenesis

Cite this

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AU - Chen, Ruei-Ming

AU - Wu, Gong-Jhe

AU - Tai, Yu-Ting

AU - Sun, Wei Zen

AU - Lin, Yi Ling

AU - Jean, Wen Chi

AU - Chen, Ta-Liang

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