Promoter CpG methylation of tumor suppressor genes in colorectal cancer and its relationship to clinical features

Shyr Yi Lin, Kun Tu Yeh, Willian Tzu Liang Chen, Hung Chang Chen, Shou Tung Chen, Hung Yi Chiou, Jan Gowth Chang

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39 Citations (Scopus)


Aberrant promoter methylation of CpG islands of tumor suppressor genes inhibits expression of the genes and may lead to tumorigenesis. We investigated the aberrant methylation profile of potential tumor suppressor genes of p15, p16, SOCS-1, and Wnt signaling pathway in colorectal cancers and correlated the data with clinical findings. Cancerous and nearby non-cancerous tissues of 185 sporadic colorectal cancer samples were studied. Methylation specific PCR was performed to explore the mechanism of inactivation in p15, p16, SOCS-1, E-cadherin, APC, GSK-3β, and Axin1 genes. Aberrant promoter methylation in p15, p16, SOCS-1, E-cadherin, APC, GSK-3β, and Axin1 genes were 5.9, 7.0, 3.8, 5.9, 12.4, 2.2, and 0% for cancerous tissues, respectively, whereas the frequencies were 3.8, 0, 0, 7.0, 2.7, 0.5, and 0% for nearby non-cancerous tissues, respectively. The frequency of aberrant promoter methylation of cancerous tissues was significant higher than non-cancerous tissues in p16, SOCS-1, and APC genes (p

Original languageEnglish
Pages (from-to)341-348
Number of pages8
JournalOncology Reports
Issue number2
Publication statusPublished - Feb 2004



  • Direct sequencing
  • Immunohistochemistry
  • Methylation specific PCR
  • Multiple methylation phenotype
  • Tumor suppressor genes

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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