Promoter CpG methylation caveolin-1 in sporadic colorectal cancer

Shyr Yi Lin, Kun Tu Yeh, Willian Tzu Liang Chen, Hung Chang Chen, Shou Tung Chen, Jan Gowth Chang

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Background: Caveolin-1 has been shown to be down-regulated in human colon cancer and involved in colon tumorigenesis. We investigated the mechanism. Materials and Methods: Cancerous and nearby non-cancerous tissues of 185 sporadic colorectal cancer samples were enrolled in this study. Methylation-specific PCR was performed to explore the mechanism of regulation of caveolin-1 gene expression. Results: Aberrant promoter methylation in the caveolin-1 gene was 3.8% and 5.9% for cancerous and nearby non-cancerous tissues, respectively. All the cancerous and non-cancerous tissue contained unmethylated promoters in the caveolin-1 gene. The methylation status of caveolin-1 had no clear relationship with age, cell grade, location of tumor or lymph node metastasis. However, female gender showed statistically significant difference (p=0.045). The immunohistochemistry study demonstrated that expression of caveolin-1 correlated with aberrant promoter methylation status in sporadic colorectal cancer tissues. Conclusion: Our findings suggested that aberrant promoter methylation of the caveolin-1 gene may occur at the precancerous stage, regulated by gender-related factors and is associated with gene silencing of caveolin-1 in the development of colorectal cancer.

Original languageEnglish
Pages (from-to)1645-1650
Number of pages6
JournalAnticancer Research
Volume24
Issue number3 A
Publication statusPublished - May 2004

Fingerprint

Caveolin 1
Methylation
Colorectal Neoplasms
Genes
Gene Silencing
Colonic Neoplasms
Colon
Carcinogenesis
Lymph Nodes
Immunohistochemistry
Neoplasm Metastasis
Gene Expression
Polymerase Chain Reaction

Keywords

  • Caveolin-1 gene
  • Colorectal cancer
  • Methylation specific PCR

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Lin, S. Y., Yeh, K. T., Chen, W. T. L., Chen, H. C., Chen, S. T., & Chang, J. G. (2004). Promoter CpG methylation caveolin-1 in sporadic colorectal cancer. Anticancer Research, 24(3 A), 1645-1650.

Promoter CpG methylation caveolin-1 in sporadic colorectal cancer. / Lin, Shyr Yi; Yeh, Kun Tu; Chen, Willian Tzu Liang; Chen, Hung Chang; Chen, Shou Tung; Chang, Jan Gowth.

In: Anticancer Research, Vol. 24, No. 3 A, 05.2004, p. 1645-1650.

Research output: Contribution to journalArticle

Lin, SY, Yeh, KT, Chen, WTL, Chen, HC, Chen, ST & Chang, JG 2004, 'Promoter CpG methylation caveolin-1 in sporadic colorectal cancer', Anticancer Research, vol. 24, no. 3 A, pp. 1645-1650.
Lin SY, Yeh KT, Chen WTL, Chen HC, Chen ST, Chang JG. Promoter CpG methylation caveolin-1 in sporadic colorectal cancer. Anticancer Research. 2004 May;24(3 A):1645-1650.
Lin, Shyr Yi ; Yeh, Kun Tu ; Chen, Willian Tzu Liang ; Chen, Hung Chang ; Chen, Shou Tung ; Chang, Jan Gowth. / Promoter CpG methylation caveolin-1 in sporadic colorectal cancer. In: Anticancer Research. 2004 ; Vol. 24, No. 3 A. pp. 1645-1650.
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