Promoter CpG methylation caveolin-1 in sporadic colorectal cancer

Shyr Yi Lin, Kun Tu Yeh, Willian Tzu Liang Chen, Hung Chang Chen, Shou Tung Chen, Jan Gowth Chang

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Background: Caveolin-1 has been shown to be down-regulated in human colon cancer and involved in colon tumorigenesis. We investigated the mechanism. Materials and Methods: Cancerous and nearby non-cancerous tissues of 185 sporadic colorectal cancer samples were enrolled in this study. Methylation-specific PCR was performed to explore the mechanism of regulation of caveolin-1 gene expression. Results: Aberrant promoter methylation in the caveolin-1 gene was 3.8% and 5.9% for cancerous and nearby non-cancerous tissues, respectively. All the cancerous and non-cancerous tissue contained unmethylated promoters in the caveolin-1 gene. The methylation status of caveolin-1 had no clear relationship with age, cell grade, location of tumor or lymph node metastasis. However, female gender showed statistically significant difference (p=0.045). The immunohistochemistry study demonstrated that expression of caveolin-1 correlated with aberrant promoter methylation status in sporadic colorectal cancer tissues. Conclusion: Our findings suggested that aberrant promoter methylation of the caveolin-1 gene may occur at the precancerous stage, regulated by gender-related factors and is associated with gene silencing of caveolin-1 in the development of colorectal cancer.

Original languageEnglish
Pages (from-to)1645-1650
Number of pages6
JournalAnticancer Research
Volume24
Issue number3 A
Publication statusPublished - May 2004

Keywords

  • Caveolin-1 gene
  • Colorectal cancer
  • Methylation specific PCR

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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    Lin, S. Y., Yeh, K. T., Chen, W. T. L., Chen, H. C., Chen, S. T., & Chang, J. G. (2004). Promoter CpG methylation caveolin-1 in sporadic colorectal cancer. Anticancer Research, 24(3 A), 1645-1650.