Promising urinary miRNA biomarkers t-SPR profiling for urothelial cell carcinoma

Wing Kiu Yeung, Hsiao Fen Li, Cheng Lin Chung, Kuang Li Lee, Pei Kuen Wei, Heng Lin, Hsi Hsien Chen, Ji Yen Cheng

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

An integrated transmission surface plasmon resonance platform with microfluidic sensor has a high potential to be a medical diagnostic tool. There has been extensive research into the potential use of transmission surface plasmon resonance, but here we demonstrate the practical application of transmission surface plasmon resonance to monitor and compare the urinary microRNA expression between the urothelial cell carcinoma patient group and the healthy control group. Furthermore, the expression levels are compared with the conventional real-time quantitative polymerase chain reaction method to verify the results. The relative expression of miR-21–3p and miR–214–3p have demonstrated to be upregulated in urothelial cell carcinoma patient group when measured using both real-time quantitative polymerase chain reaction and transmission surface plasmon resonance. Using receiver operating characteristics of miR-21–3p and miR-214–3p the area under curve reaches an excellent score of 0.99 and 0.917, respectively. Our work has demonstrated the ability of clinical application of transmission surface plasmon resonance as a rapid and cost effective point-of-care.

Original languageEnglish
Article number128605
JournalSensors and Actuators, B: Chemical
Volume322
DOIs
Publication statusPublished - Nov 1 2020

Keywords

  • Marker
  • MicroRNA
  • T-SPR
  • Tumour
  • Urine
  • Urothelial cell carcinoma

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Instrumentation
  • Condensed Matter Physics
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Electrical and Electronic Engineering
  • Materials Chemistry

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