Proliferation of retinal pigment epithelial cells induced by (R, R)-XY-10 and (S, S)-XY-10 and their action mechanisms

Yu Wen Cheng, Yu Liang Wang, Yi Hua Zhang, Si Xun Peng, George C.Y. Chiou

Research output: Contribution to journalArticlepeer-review

Abstract

• AIM: To investigate the mechanism of proliferation effect induced by (R, R)-XY-10 and (S, S)-XY-10 on retinal pigmented epithelial cells (ARPE-19). • METHODS: Human retinal pigmented epithelial cells (ARPE-19) and human umbilical vein endothelial cells (HUVECs) were used to investigate the effect of (R, R)-XY-10 and (S, S)-XY-10 on cell growth, and their mechanisms of proliferative action by using ERKinverted commas AKTinverted commas PI3Kinverted commas Protein kinase C (PKC)and Nitric oxide synthase (NOS) inhibitors. RESULTS: (R, R)-XY-10 and (S, S)-XY-10 dose-dependently increased ARPE-19 cell proliferation, but not on HUVECs. When treated with proliferative inhibitors, H7 (5μ mol/L)inverted commas hypericin (20μ mol/L)inverted commas PD98059 (2μ mol/L)inverted commas LY294002 (50μ mol/L)inverted commas SH-5 (10μ mol/L) and L-NAME (100μ mol/L), the proliferative effect was reduced by H7inverted commas hypericininverted commas PD98059 and LY294002, but not by SH-5 and L-NAME. • CONCLUSION: (R, R)-XY-10 and (S, S)-XY-10 can induce cell proliferation through MAPK and PI3K dependent pathway.

Original languageEnglish
Pages (from-to)1641-1645
Number of pages5
JournalInternational Journal of Ophthalmology
Volume9
Issue number9
DOIs
Publication statusPublished - Oct 22 2009
Externally publishedYes

Keywords

  • (R, R)-XY-10
  • (S, S)-XY-10
  • Age-related macular degeneration
  • ARPE-19 cells
  • Human umbilical vein endothelial cells
  • Proliferation

ASJC Scopus subject areas

  • Ophthalmology

Fingerprint Dive into the research topics of 'Proliferation of retinal pigment epithelial cells induced by (R, R)-XY-10 and (S, S)-XY-10 and their action mechanisms'. Together they form a unique fingerprint.

Cite this