Proliferation of retinal pigment epithelial cells induced by (R, R)-XY-10 and (S, S)-XY-10 and their action mechanisms

Yu Wen Cheng, Yu Liang Wang, Yi Hua Zhang, Si Xun Peng, George C.Y. Chiou

Research output: Contribution to journalArticle

Abstract

• AIM: To investigate the mechanism of proliferation effect induced by (R, R)-XY-10 and (S, S)-XY-10 on retinal pigmented epithelial cells (ARPE-19). • METHODS: Human retinal pigmented epithelial cells (ARPE-19) and human umbilical vein endothelial cells (HUVECs) were used to investigate the effect of (R, R)-XY-10 and (S, S)-XY-10 on cell growth, and their mechanisms of proliferative action by using ERKinverted commas AKTinverted commas PI3Kinverted commas Protein kinase C (PKC)and Nitric oxide synthase (NOS) inhibitors. RESULTS: (R, R)-XY-10 and (S, S)-XY-10 dose-dependently increased ARPE-19 cell proliferation, but not on HUVECs. When treated with proliferative inhibitors, H7 (5μ mol/L)inverted commas hypericin (20μ mol/L)inverted commas PD98059 (2μ mol/L)inverted commas LY294002 (50μ mol/L)inverted commas SH-5 (10μ mol/L) and L-NAME (100μ mol/L), the proliferative effect was reduced by H7inverted commas hypericininverted commas PD98059 and LY294002, but not by SH-5 and L-NAME. • CONCLUSION: (R, R)-XY-10 and (S, S)-XY-10 can induce cell proliferation through MAPK and PI3K dependent pathway.

Original languageEnglish
Pages (from-to)1641-1645
Number of pages5
JournalInternational Journal of Ophthalmology
Volume9
Issue number9
DOIs
Publication statusPublished - Oct 22 2009
Externally publishedYes

Fingerprint

2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one
Retinal Pigments
NG-Nitroarginine Methyl Ester
Human Umbilical Vein Endothelial Cells
Epithelial Cells
Cell Proliferation
Phosphatidylinositol 3-Kinases
Nitric Oxide Synthase
Protein Kinase C
Growth
2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one
hypericin

Keywords

  • (R, R)-XY-10
  • (S, S)-XY-10
  • Age-related macular degeneration
  • ARPE-19 cells
  • Human umbilical vein endothelial cells
  • Proliferation

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Proliferation of retinal pigment epithelial cells induced by (R, R)-XY-10 and (S, S)-XY-10 and their action mechanisms. / Cheng, Yu Wen; Wang, Yu Liang; Zhang, Yi Hua; Peng, Si Xun; Chiou, George C.Y.

In: International Journal of Ophthalmology, Vol. 9, No. 9, 22.10.2009, p. 1641-1645.

Research output: Contribution to journalArticle

Cheng, Yu Wen ; Wang, Yu Liang ; Zhang, Yi Hua ; Peng, Si Xun ; Chiou, George C.Y. / Proliferation of retinal pigment epithelial cells induced by (R, R)-XY-10 and (S, S)-XY-10 and their action mechanisms. In: International Journal of Ophthalmology. 2009 ; Vol. 9, No. 9. pp. 1641-1645.
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T1 - Proliferation of retinal pigment epithelial cells induced by (R, R)-XY-10 and (S, S)-XY-10 and their action mechanisms

AU - Cheng, Yu Wen

AU - Wang, Yu Liang

AU - Zhang, Yi Hua

AU - Peng, Si Xun

AU - Chiou, George C.Y.

PY - 2009/10/22

Y1 - 2009/10/22

N2 - • AIM: To investigate the mechanism of proliferation effect induced by (R, R)-XY-10 and (S, S)-XY-10 on retinal pigmented epithelial cells (ARPE-19). • METHODS: Human retinal pigmented epithelial cells (ARPE-19) and human umbilical vein endothelial cells (HUVECs) were used to investigate the effect of (R, R)-XY-10 and (S, S)-XY-10 on cell growth, and their mechanisms of proliferative action by using ERKinverted commas AKTinverted commas PI3Kinverted commas Protein kinase C (PKC)and Nitric oxide synthase (NOS) inhibitors. RESULTS: (R, R)-XY-10 and (S, S)-XY-10 dose-dependently increased ARPE-19 cell proliferation, but not on HUVECs. When treated with proliferative inhibitors, H7 (5μ mol/L)inverted commas hypericin (20μ mol/L)inverted commas PD98059 (2μ mol/L)inverted commas LY294002 (50μ mol/L)inverted commas SH-5 (10μ mol/L) and L-NAME (100μ mol/L), the proliferative effect was reduced by H7inverted commas hypericininverted commas PD98059 and LY294002, but not by SH-5 and L-NAME. • CONCLUSION: (R, R)-XY-10 and (S, S)-XY-10 can induce cell proliferation through MAPK and PI3K dependent pathway.

AB - • AIM: To investigate the mechanism of proliferation effect induced by (R, R)-XY-10 and (S, S)-XY-10 on retinal pigmented epithelial cells (ARPE-19). • METHODS: Human retinal pigmented epithelial cells (ARPE-19) and human umbilical vein endothelial cells (HUVECs) were used to investigate the effect of (R, R)-XY-10 and (S, S)-XY-10 on cell growth, and their mechanisms of proliferative action by using ERKinverted commas AKTinverted commas PI3Kinverted commas Protein kinase C (PKC)and Nitric oxide synthase (NOS) inhibitors. RESULTS: (R, R)-XY-10 and (S, S)-XY-10 dose-dependently increased ARPE-19 cell proliferation, but not on HUVECs. When treated with proliferative inhibitors, H7 (5μ mol/L)inverted commas hypericin (20μ mol/L)inverted commas PD98059 (2μ mol/L)inverted commas LY294002 (50μ mol/L)inverted commas SH-5 (10μ mol/L) and L-NAME (100μ mol/L), the proliferative effect was reduced by H7inverted commas hypericininverted commas PD98059 and LY294002, but not by SH-5 and L-NAME. • CONCLUSION: (R, R)-XY-10 and (S, S)-XY-10 can induce cell proliferation through MAPK and PI3K dependent pathway.

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KW - (S, S)-XY-10

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KW - (R, R)-XY-10

KW - (S, S)-XY-10

KW - Age-related macular degeneration

KW - ARPE-19 cells

KW - Human umbilical vein endothelial cells

KW - Proliferation

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