Progesterone inhibits endothelial cell migration through suppression of the rho activity mediated by csrc activation

Tong Sheng Lee, Jhen Jhe Lin, Yen Nien Huo, Wen Sen Lee

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

We previously showed that progesterone (P4) could inhibit the proliferation of human umbilical venous endothelial cells (HUVECs) through the p53-dependent pathway. In the present study, we further demonstrated that P4 at physiologic levels (5-500 nM) concentration-dependently inhibited migration of HUVECs. This effect was blocked by pre-treatment with the P4 receptor (PR) agonist-antagonist, RU486, suggesting that the P4-induced migration inhibition in HUVECs was through the PR-mediated signaling pathway. Western blot analyses demonstrated that the levels of RhoA and Rac-1 protein were reduced in the P4-treated HUVECs. P4 also inhibited the membrane translocation of RhoA and Rac-1 protein. Moreover, the P4-induced migration inhibition in HUVECs was prevented by over-expression of the constitutively active RhoA construct (RhoA V14). However, pre-treatment with the ROCK (a kinase associated with RhoA for transducing RhoA signaling) inhibitor, Y27632, abolished the over-expression of RhoA-induced prevention effect on the P4-induced migration inhibition in HUVECs. These data suggest that the inhibition of Rho GTPases might account for the P4-induced migration inhibition of HUVECs. Pre-treatment with the cSrc inhibitor, PP2, prevented the P4-induced migration inhibition in HUVEC. The levels of phosphorylated focal adhesion kinase (FAK) and paxillin protein were also decreased by P4 treatment. Taken together, these results suggest that suppression of the Rho-mediated pathway might be involved in the signal transduction leading to the inhibition of cell migration caused by P4 in HUVECs. J. Cell. Biochem. 116: 1411-1418, 2015.

Original languageEnglish
Pages (from-to)1411-1418
Number of pages8
JournalJournal of Cellular Biochemistry
Volume116
Issue number7
DOIs
Publication statusPublished - Jul 1 2015

Fingerprint

Umbilicus
Endothelial cells
Cell Movement
Progesterone
Endothelial Cells
Chemical activation
rac GTP-Binding Proteins
Cell Migration Inhibition
Paxillin
Focal Adhesion Protein-Tyrosine Kinases
Signal transduction
rho GTP-Binding Proteins
Proteins
Signal Transduction
Phosphotransferases
Western Blotting
Cells
Membranes

Keywords

  • ANGIOGENESIS
  • PROGESTERONE RECEPTOR
  • RHO PROTEINS

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

Progesterone inhibits endothelial cell migration through suppression of the rho activity mediated by csrc activation. / Lee, Tong Sheng; Lin, Jhen Jhe; Huo, Yen Nien; Lee, Wen Sen.

In: Journal of Cellular Biochemistry, Vol. 116, No. 7, 01.07.2015, p. 1411-1418.

Research output: Contribution to journalArticle

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