Production of the chemokine eotaxin-1 in osteoarthritis and its role in cartilage degradation

Yi Hsin Hsu, Ming-Shium Hsieh, Yu Chih Liang, Chao Yi Li, Ming Thau Sheu, Der Tsay Chou, Tzeng-Fu Chen, Chien Ho Chen

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Abstract

The expression of the chemokine, eotaxin-1, and its receptors in normal and osteoarthritic human chondrocytes was examined, and its role in cartilage degradation was elucidated in this study. Results indicated that plasma concentrations of eotaxin-1 as well as the chemokines, RANTES, and MCP-1α, were higher in patients with osteoarthritis (OA) than those in normal humans. Stimulation of chondrocytes with IL-1β or TNF-α significantly induced eotaxin-1 expression. The production of eotaxin-1 induced expression of its own receptor of CCR3 and CCR5 on the cell surface of chondrosarcomas, suggesting that an autocrine/paracrine pathway is involved in eotaxin-1's action. In addition, eotaxin-1 markedly increased the expressions of MMP-3 and MMP-13 mRNA, but had no effect on TIMP-1 expression in chondrocytes. However, pretreatment of anti-eotaxin-1 antibody significantly decreased the MMP-3 expression induced by IL-1β. These results first demonstrate that human chondrocytes express the chemokine, eotaxin-1, and that its expression is induced by treatment with IL-1β and TNF-α. The cytokine-triggered induction of eotaxin-1 further results in enhanced expressions of its own receptor of CCR3, CCR5, and MMPs, suggesting that eotaxin-1 plays an important role in cartilage degradation in OA.

Original languageEnglish
Pages (from-to)929-939
Number of pages11
JournalJournal of Cellular Biochemistry
Volume93
Issue number5
DOIs
Publication statusPublished - 2004

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Keywords

  • Chemokine
  • Chondrocyte
  • Eotaxin-1
  • Matrix metalloproteinase
  • Osteoarthritis

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology

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