Production of macrophage inflammatory protein-2 following hypoxia/reoxygenation in glial cells

Ju Yu Wang, Andrew Y C Shum, Chun C. Chao, Jon S. Kuo, Jia Yi Wang

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Polymorphonuclear neutrophils (PMNs) are known to mediate brain inflammation following hypoxia/reoxygenation (H/R), but the precise mechanisms leading to PMN recruitment are undefined. The α-chemokine macrophage inflammatory protein-2 (MIP-2) has specificity for the recruitment of PMNs. In this study, we found that 8 or 12 h of hypoxia followed by 24-h reoxygenation (H8/R24 or H12/R24) induced MIP-2 secretion in cultures of enriched microglia or mixed glia, respectively. Microglia, however, could not survive longer duration (>12 h) of hypoxia. Astrocytes did not produce any significant amount of MIP-2 even though astrocytes maintained 98-99% viability following H12/R24. We also found that microglia survived the H/R treatment better (following H24) in the presence of astrocytes (mixed glial culture) than in microglia-enriched culture. Reoxygenation for prolonged periods (3 and 5 days) following H24 resulted in progressively larger increases in MIP-2 production (20- and 60-fold, respectively) in mixed glial cultures. Immunocytochemical staining revealed that the cells expressing MIP-2 in response to H/R were microglia rather than astrocytes in mixed glial cultures. Examination of MIP-2 mRNA expression showed that H/R upregulated MIP-2 gene expression. Taken together, our data suggest that microglial cells are an important source of MIP-2 production and suggest a potential injury mechanism involving brain-derived production of MIP-2 in H/R. (C) 2000 Wiley-Liss, Inc.

Original languageEnglish
Pages (from-to)155-164
Number of pages10
JournalGLIA
Volume32
Issue number2
DOIs
Publication statusPublished - 2000
Externally publishedYes

Fingerprint

Chemokine CXCL2
Neuroglia
Microglia
Astrocytes
Neutrophil Infiltration
Hypoxia
Encephalitis
Chemokines
Neutrophils
Staining and Labeling
Gene Expression

Keywords

  • Chemokine
  • Hypoxia/reoxygenation
  • Microglia
  • MIP-2

ASJC Scopus subject areas

  • Immunology

Cite this

Production of macrophage inflammatory protein-2 following hypoxia/reoxygenation in glial cells. / Wang, Ju Yu; Shum, Andrew Y C; Chao, Chun C.; Kuo, Jon S.; Wang, Jia Yi.

In: GLIA, Vol. 32, No. 2, 2000, p. 155-164.

Research output: Contribution to journalArticle

Wang, Ju Yu ; Shum, Andrew Y C ; Chao, Chun C. ; Kuo, Jon S. ; Wang, Jia Yi. / Production of macrophage inflammatory protein-2 following hypoxia/reoxygenation in glial cells. In: GLIA. 2000 ; Vol. 32, No. 2. pp. 155-164.
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