Probiotics have clinical, microbiologic, and immunologic efficacy in acute infectious diarrhea

Chien Chang Chen, Man Shan Kong, Ming Wei Lai, Hsun Chin Chao, Kuei Wen Chang, Shih Yen Chen, Yhu Chering Huang, Cheng Hsun Chiu, Wen Chen Li, Pen Yi Lin, Chih Jung Chen, Tzou Yien Lin

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

Background: Acute infectious diarrhea is a major cause of childhood morbidity and economic burden for families. We evaluate the clinical, microbiologic, and immunologic effects of probiotics in acute infectious diarrhea. Methods: Children (n = 304) aged 3 months to 6 years hospitalized for acute diarrhea were randomized to receive Bio-three (a mixture of Bacillus mesentericus, Enterococcus faecalis, and Clostridium butyricum) or placebo orally 3 times daily for 7 days. Fecal samples were homogenized for bacterial culture and blood cells were isolated for cell culture and cytokine analysis. This study is registered (NCT00463190). Results: The mean duration of diarrhea after start of therapy was 60.1 hours in the probiotics group versus 86.3 hours in the placebo group (P = 0.003). Hospital stay was shorter in the probiotics group than in the placebo group (P = 0.009). Counts of Bifidobacteria and Lactobacillus species were elevated in stool culture of the probiotics (Bio-three) group. IL-10 was increased in the serum and supernatants of cell culture in the probiotics group, and tumor necrosis factor-α values were down-regulated. Interferon-γ and IL-12 were mildly elevated in the probiotics group, compared with the placebo group. Conclusions: This probiotics mixture reduced the severity of diarrhea and length of hospital stay in children with acute diarrhea. In addition to restoring beneficial intestinal flora, probiotics may enhance host protective immunity such as down-regulation of pro-inflammatory cytokines and up-regulation of anti-inflammatory cytokines.

Original languageEnglish
Pages (from-to)135-138
Number of pages4
JournalPediatric Infectious Disease Journal
Volume29
Issue number2
DOIs
Publication statusPublished - Feb 2010
Externally publishedYes

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Probiotics
Diarrhea
Placebos
Length of Stay
Cytokines
Clostridium butyricum
Cell Culture Techniques
Bifidobacterium
Enterococcus faecalis
Lactobacillus
Interleukin-12
Interleukin-10
Bacillus
Interferons
Immunity
Blood Cells
Anti-Inflammatory Agents
Up-Regulation
Down-Regulation
Tumor Necrosis Factor-alpha

Keywords

  • Bacillus mesentericus
  • Bio-three
  • Clostridium butyricum
  • Enterococcus faecalis
  • Peripheral blood mononuclear cells
  • Probiotics

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health
  • Microbiology (medical)
  • Infectious Diseases

Cite this

Probiotics have clinical, microbiologic, and immunologic efficacy in acute infectious diarrhea. / Chen, Chien Chang; Kong, Man Shan; Lai, Ming Wei; Chao, Hsun Chin; Chang, Kuei Wen; Chen, Shih Yen; Huang, Yhu Chering; Chiu, Cheng Hsun; Li, Wen Chen; Lin, Pen Yi; Chen, Chih Jung; Lin, Tzou Yien.

In: Pediatric Infectious Disease Journal, Vol. 29, No. 2, 02.2010, p. 135-138.

Research output: Contribution to journalArticle

Chen, CC, Kong, MS, Lai, MW, Chao, HC, Chang, KW, Chen, SY, Huang, YC, Chiu, CH, Li, WC, Lin, PY, Chen, CJ & Lin, TY 2010, 'Probiotics have clinical, microbiologic, and immunologic efficacy in acute infectious diarrhea', Pediatric Infectious Disease Journal, vol. 29, no. 2, pp. 135-138. https://doi.org/10.1097/INF.0b013e3181b530bf
Chen, Chien Chang ; Kong, Man Shan ; Lai, Ming Wei ; Chao, Hsun Chin ; Chang, Kuei Wen ; Chen, Shih Yen ; Huang, Yhu Chering ; Chiu, Cheng Hsun ; Li, Wen Chen ; Lin, Pen Yi ; Chen, Chih Jung ; Lin, Tzou Yien. / Probiotics have clinical, microbiologic, and immunologic efficacy in acute infectious diarrhea. In: Pediatric Infectious Disease Journal. 2010 ; Vol. 29, No. 2. pp. 135-138.
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AU - Chen, Shih Yen

AU - Huang, Yhu Chering

AU - Chiu, Cheng Hsun

AU - Li, Wen Chen

AU - Lin, Pen Yi

AU - Chen, Chih Jung

AU - Lin, Tzou Yien

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N2 - Background: Acute infectious diarrhea is a major cause of childhood morbidity and economic burden for families. We evaluate the clinical, microbiologic, and immunologic effects of probiotics in acute infectious diarrhea. Methods: Children (n = 304) aged 3 months to 6 years hospitalized for acute diarrhea were randomized to receive Bio-three (a mixture of Bacillus mesentericus, Enterococcus faecalis, and Clostridium butyricum) or placebo orally 3 times daily for 7 days. Fecal samples were homogenized for bacterial culture and blood cells were isolated for cell culture and cytokine analysis. This study is registered (NCT00463190). Results: The mean duration of diarrhea after start of therapy was 60.1 hours in the probiotics group versus 86.3 hours in the placebo group (P = 0.003). Hospital stay was shorter in the probiotics group than in the placebo group (P = 0.009). Counts of Bifidobacteria and Lactobacillus species were elevated in stool culture of the probiotics (Bio-three) group. IL-10 was increased in the serum and supernatants of cell culture in the probiotics group, and tumor necrosis factor-α values were down-regulated. Interferon-γ and IL-12 were mildly elevated in the probiotics group, compared with the placebo group. Conclusions: This probiotics mixture reduced the severity of diarrhea and length of hospital stay in children with acute diarrhea. In addition to restoring beneficial intestinal flora, probiotics may enhance host protective immunity such as down-regulation of pro-inflammatory cytokines and up-regulation of anti-inflammatory cytokines.

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