Probiotics-Derived Extracellular Vesicles Protect Oxidative Stress against H2 O2 Induction in Placental Cells

Le Ming Wang, Bao Hong Lee, Chih Yao Hou, Wei Hsuan Hsu, Chen Jei Tai

Research output: Contribution to journalArticlepeer-review

Abstract

(1) Background: The microbial phase in the vaginal environment has been found to regulate the physiological activity of host cells. Studies have demonstrated that abnormal microbial growth in the vagina and a significant reduction in the proportion of lactic acid bacteria promote the occurrence of spontaneous preterm birth (sPTB). However, the contributing mechanism remains unknown. (2) Methods: This study uses extracellular vesicles (EVs) secreted by the probiotic Lactobacillus crispatus, commonly found in the vagina, to explore their potential to attenuate placental cells caused by oxidative stress induction. (3) Results: We found that L. crispatus-derived EVs improved Akt phosphorylation and attenuated both cell senescence and death in placental cells caused by oxidative stress induction. In addition, L. crispatus-derived EVs enhanced the resistance to H2 O2 induction mediated by increasing mitochondrial fusion. (4) Conclusion: This is the first study to demonstrate that L. crispatus in the vagina can not only regulate the physiological functions of placental cells through the delivery of L. crispatus-EVs but also reduce cell senescence. As cell senescence is related to the occurrence of sPTB, these results indicate that maintaining the population of L. crispatus in the vaginal environment should be an adjuvant treatment strategy to avoid sPTB.

Original languageEnglish
Article number74
JournalFermentation
Volume8
Issue number2
DOIs
Publication statusPublished - Feb 2022

Keywords

  • Extracellular vesicles (EVs)
  • Lactobacillus crispatus
  • Mitochondrial fusion
  • Senescence
  • Spontaneous preterm birth (sPTB)

ASJC Scopus subject areas

  • Food Science
  • Biochemistry, Genetics and Molecular Biology (miscellaneous)
  • Plant Science

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