Probiotic Bio-Three induces Th1 and anti-inflammatory effects in PBMC and dendritic cells

Man Chin Hua, Tzou Yien Lin, Ming Wei Lai, Man Shan Kong, Hung Ju Chang, Chien Chang Chen

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

AIM: To investigate the immune response of peripheral blood mononuclear cells (PBMCs) and dendritic cells (DCs) that were stimulated by probiotic preparations. METHODS: PBMCs were isolated, cultured, and stimulated with Bio-Three (a mixture of Bacillus mesentericus, Clostridium butyricum and Enterococcus faecalis; 105, 106 and 107 CFU/mL for 24 h). Cytokine production of (1) circulating PBMCs; (2) PBMCs stimulated by probiotic preparation; (3) monocyte-derived DCs; and (4) DC and T cell co-culture was determined by enzyme-linked immunosorbent assay. Phenotypic analysis of circulating PBMCs was also investigated by flow cytometry. Blood was obtained from individuals who consumed Bio-Three (109 CFU/d B. mesentericus, C. butyricum and E. faecalis) for 2 wk, or those who did not take probiotics orally. RESULTS: In culture supernatants, interferon-γ (IFN-γ) and interleukin (IL)-10 production increased, but IL-4 and tumor necrosis factor-α (TNF-α) production by PBMCs decreased after 1 and 2 wk of probiotic treatment. Flow cytometry was also performed on day 14 and detected enhanced expression of CD11b, HLA-DR, CD4, CD45RA, CD25, CD44 and CD69 in response to Bio-Three. Furthermore, IL-10 and IL-12 were upregulated in supernatants of monocyte-derived DCs, and IFN-γ and IL-10 were enhanced in supernatants of CD4+ T cells co-cultured with DCs. CONCLUSION: Bio-Three appeared to stimulate the Th1 immune response, downregulate pro-inflammatory cytokines (TNF-α) and upregulate anti-inflammatory cytokine (IL-10). Probiotics could be effective in activation of PBMCs and DCs.

Original languageEnglish
Pages (from-to)3529-3540
Number of pages12
JournalWorld Journal of Gastroenterology
Volume16
Issue number28
DOIs
Publication statusPublished - Jul 28 2010
Externally publishedYes

Fingerprint

Probiotics
Dendritic Cells
Blood Cells
Anti-Inflammatory Agents
Interleukin-10
Clostridium butyricum
Enterococcus faecalis
Cytokines
Interferons
Monocytes
Flow Cytometry
Tumor Necrosis Factor-alpha
T-Lymphocytes
HLA-DR Antigens
Interleukin-12
Coculture Techniques
Interleukin-4
Bacillus
Up-Regulation
Down-Regulation

Keywords

  • Bio-Three
  • Dendritic cells
  • Peripheral blood mononuclear cells
  • Probiotics

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Probiotic Bio-Three induces Th1 and anti-inflammatory effects in PBMC and dendritic cells. / Hua, Man Chin; Lin, Tzou Yien; Lai, Ming Wei; Kong, Man Shan; Chang, Hung Ju; Chen, Chien Chang.

In: World Journal of Gastroenterology, Vol. 16, No. 28, 28.07.2010, p. 3529-3540.

Research output: Contribution to journalArticle

Hua, Man Chin ; Lin, Tzou Yien ; Lai, Ming Wei ; Kong, Man Shan ; Chang, Hung Ju ; Chen, Chien Chang. / Probiotic Bio-Three induces Th1 and anti-inflammatory effects in PBMC and dendritic cells. In: World Journal of Gastroenterology. 2010 ; Vol. 16, No. 28. pp. 3529-3540.
@article{b2208da92ddd4a13ac98117ec4a6ea92,
title = "Probiotic Bio-Three induces Th1 and anti-inflammatory effects in PBMC and dendritic cells",
abstract = "AIM: To investigate the immune response of peripheral blood mononuclear cells (PBMCs) and dendritic cells (DCs) that were stimulated by probiotic preparations. METHODS: PBMCs were isolated, cultured, and stimulated with Bio-Three (a mixture of Bacillus mesentericus, Clostridium butyricum and Enterococcus faecalis; 105, 106 and 107 CFU/mL for 24 h). Cytokine production of (1) circulating PBMCs; (2) PBMCs stimulated by probiotic preparation; (3) monocyte-derived DCs; and (4) DC and T cell co-culture was determined by enzyme-linked immunosorbent assay. Phenotypic analysis of circulating PBMCs was also investigated by flow cytometry. Blood was obtained from individuals who consumed Bio-Three (109 CFU/d B. mesentericus, C. butyricum and E. faecalis) for 2 wk, or those who did not take probiotics orally. RESULTS: In culture supernatants, interferon-γ (IFN-γ) and interleukin (IL)-10 production increased, but IL-4 and tumor necrosis factor-α (TNF-α) production by PBMCs decreased after 1 and 2 wk of probiotic treatment. Flow cytometry was also performed on day 14 and detected enhanced expression of CD11b, HLA-DR, CD4, CD45RA, CD25, CD44 and CD69 in response to Bio-Three. Furthermore, IL-10 and IL-12 were upregulated in supernatants of monocyte-derived DCs, and IFN-γ and IL-10 were enhanced in supernatants of CD4+ T cells co-cultured with DCs. CONCLUSION: Bio-Three appeared to stimulate the Th1 immune response, downregulate pro-inflammatory cytokines (TNF-α) and upregulate anti-inflammatory cytokine (IL-10). Probiotics could be effective in activation of PBMCs and DCs.",
keywords = "Bio-Three, Dendritic cells, Peripheral blood mononuclear cells, Probiotics",
author = "Hua, {Man Chin} and Lin, {Tzou Yien} and Lai, {Ming Wei} and Kong, {Man Shan} and Chang, {Hung Ju} and Chen, {Chien Chang}",
year = "2010",
month = "7",
day = "28",
doi = "10.3748/wjg.v16.i28.3529",
language = "English",
volume = "16",
pages = "3529--3540",
journal = "World Journal of Gastroenterology",
issn = "1007-9327",
publisher = "WJG Press",
number = "28",

}

TY - JOUR

T1 - Probiotic Bio-Three induces Th1 and anti-inflammatory effects in PBMC and dendritic cells

AU - Hua, Man Chin

AU - Lin, Tzou Yien

AU - Lai, Ming Wei

AU - Kong, Man Shan

AU - Chang, Hung Ju

AU - Chen, Chien Chang

PY - 2010/7/28

Y1 - 2010/7/28

N2 - AIM: To investigate the immune response of peripheral blood mononuclear cells (PBMCs) and dendritic cells (DCs) that were stimulated by probiotic preparations. METHODS: PBMCs were isolated, cultured, and stimulated with Bio-Three (a mixture of Bacillus mesentericus, Clostridium butyricum and Enterococcus faecalis; 105, 106 and 107 CFU/mL for 24 h). Cytokine production of (1) circulating PBMCs; (2) PBMCs stimulated by probiotic preparation; (3) monocyte-derived DCs; and (4) DC and T cell co-culture was determined by enzyme-linked immunosorbent assay. Phenotypic analysis of circulating PBMCs was also investigated by flow cytometry. Blood was obtained from individuals who consumed Bio-Three (109 CFU/d B. mesentericus, C. butyricum and E. faecalis) for 2 wk, or those who did not take probiotics orally. RESULTS: In culture supernatants, interferon-γ (IFN-γ) and interleukin (IL)-10 production increased, but IL-4 and tumor necrosis factor-α (TNF-α) production by PBMCs decreased after 1 and 2 wk of probiotic treatment. Flow cytometry was also performed on day 14 and detected enhanced expression of CD11b, HLA-DR, CD4, CD45RA, CD25, CD44 and CD69 in response to Bio-Three. Furthermore, IL-10 and IL-12 were upregulated in supernatants of monocyte-derived DCs, and IFN-γ and IL-10 were enhanced in supernatants of CD4+ T cells co-cultured with DCs. CONCLUSION: Bio-Three appeared to stimulate the Th1 immune response, downregulate pro-inflammatory cytokines (TNF-α) and upregulate anti-inflammatory cytokine (IL-10). Probiotics could be effective in activation of PBMCs and DCs.

AB - AIM: To investigate the immune response of peripheral blood mononuclear cells (PBMCs) and dendritic cells (DCs) that were stimulated by probiotic preparations. METHODS: PBMCs were isolated, cultured, and stimulated with Bio-Three (a mixture of Bacillus mesentericus, Clostridium butyricum and Enterococcus faecalis; 105, 106 and 107 CFU/mL for 24 h). Cytokine production of (1) circulating PBMCs; (2) PBMCs stimulated by probiotic preparation; (3) monocyte-derived DCs; and (4) DC and T cell co-culture was determined by enzyme-linked immunosorbent assay. Phenotypic analysis of circulating PBMCs was also investigated by flow cytometry. Blood was obtained from individuals who consumed Bio-Three (109 CFU/d B. mesentericus, C. butyricum and E. faecalis) for 2 wk, or those who did not take probiotics orally. RESULTS: In culture supernatants, interferon-γ (IFN-γ) and interleukin (IL)-10 production increased, but IL-4 and tumor necrosis factor-α (TNF-α) production by PBMCs decreased after 1 and 2 wk of probiotic treatment. Flow cytometry was also performed on day 14 and detected enhanced expression of CD11b, HLA-DR, CD4, CD45RA, CD25, CD44 and CD69 in response to Bio-Three. Furthermore, IL-10 and IL-12 were upregulated in supernatants of monocyte-derived DCs, and IFN-γ and IL-10 were enhanced in supernatants of CD4+ T cells co-cultured with DCs. CONCLUSION: Bio-Three appeared to stimulate the Th1 immune response, downregulate pro-inflammatory cytokines (TNF-α) and upregulate anti-inflammatory cytokine (IL-10). Probiotics could be effective in activation of PBMCs and DCs.

KW - Bio-Three

KW - Dendritic cells

KW - Peripheral blood mononuclear cells

KW - Probiotics

UR - http://www.scopus.com/inward/record.url?scp=77955098138&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77955098138&partnerID=8YFLogxK

U2 - 10.3748/wjg.v16.i28.3529

DO - 10.3748/wjg.v16.i28.3529

M3 - Article

VL - 16

SP - 3529

EP - 3540

JO - World Journal of Gastroenterology

JF - World Journal of Gastroenterology

SN - 1007-9327

IS - 28

ER -