Preventive effect of methanol extract from Agrocybe cylindracea (MEAC) mushroom on the cooking oil fumes-induced expressions of cyclooxygenase-2 via suppression of nuclear factor-kappaB (NF-kB) in CL-3 cells

Lien Hui Wang, Huei Lee, Tai An Chiang, Mei Ju Chen, Shwu Yuann Lin

Research output: Contribution to journalArticle

Abstract

Methanol extract from Agrocybe cylindracea strain B (MEAC) mushroom is reported to suppress reactive oxygen species (ROS) production and DNA oxidative damage induced by cooking oil fumes (COF). This study examined the effects of MEAC on COF-stimulated expression of the cyclooxygenase-2 (COX-2) and cytochrome P450 CYP1A1 genes in human lung adenocarcinoma CL-3 cells. MEAC did not alter COF-stimulated CYP1A1 mRNA expression. However, MEAC significantly inhibited COF (100 μg/mL) stimulation of COX-2 mRNA and protein levels in a dose-dependent manner. The concentration of MEAC that decreased COF-stimulated COX-2 protein expression by 50% was 500 μg/mL. These data suggest that MEAC selectively affects particular types of the COF-mediated genes. COX-2 is reported to be regulated by the nuclear factor-kappaB (NF-kB) pathway. The gel retardation assay was used and showed that MEAC alone did not alter the basal NF-kB DNA binding activity; in the presence of COF, it dose-dependently suppressed COF-increased activity of NF-kB. These data suggest that MEAC may exert its anti-COF action on COX-2 gene expression via the NF-kB pathway. The results of this study support that MEAC mediates the COF-stimulated ROS production and DNA damage in CL-3 lung cancer cells.

Original languageEnglish
JournalJournal of Food and Drug Analysis
Volume19
Issue number3
Publication statusPublished - Sep 2011
Externally publishedYes

Fingerprint

Agrocybe
cooking fats and oils
transcription factor NF-kappa B
Agaricales
Cooking
prostaglandin synthase
Cyclooxygenase 2
mushrooms
Methanol
Oils
methanol
extracts
cells
Cytochrome P-450 CYP1A1
DNA Damage
reactive oxygen species
Reactive Oxygen Species
Messenger RNA
Electrophoretic Mobility Shift Assay
DNA

Keywords

  • Agrocybe cylindracea
  • Cooking oil fumes
  • COX-2
  • Nuclear factor-kappaB

ASJC Scopus subject areas

  • Food Science
  • Pharmacology

Cite this

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title = "Preventive effect of methanol extract from Agrocybe cylindracea (MEAC) mushroom on the cooking oil fumes-induced expressions of cyclooxygenase-2 via suppression of nuclear factor-kappaB (NF-kB) in CL-3 cells",
abstract = "Methanol extract from Agrocybe cylindracea strain B (MEAC) mushroom is reported to suppress reactive oxygen species (ROS) production and DNA oxidative damage induced by cooking oil fumes (COF). This study examined the effects of MEAC on COF-stimulated expression of the cyclooxygenase-2 (COX-2) and cytochrome P450 CYP1A1 genes in human lung adenocarcinoma CL-3 cells. MEAC did not alter COF-stimulated CYP1A1 mRNA expression. However, MEAC significantly inhibited COF (100 μg/mL) stimulation of COX-2 mRNA and protein levels in a dose-dependent manner. The concentration of MEAC that decreased COF-stimulated COX-2 protein expression by 50{\%} was 500 μg/mL. These data suggest that MEAC selectively affects particular types of the COF-mediated genes. COX-2 is reported to be regulated by the nuclear factor-kappaB (NF-kB) pathway. The gel retardation assay was used and showed that MEAC alone did not alter the basal NF-kB DNA binding activity; in the presence of COF, it dose-dependently suppressed COF-increased activity of NF-kB. These data suggest that MEAC may exert its anti-COF action on COX-2 gene expression via the NF-kB pathway. The results of this study support that MEAC mediates the COF-stimulated ROS production and DNA damage in CL-3 lung cancer cells.",
keywords = "Agrocybe cylindracea, Cooking oil fumes, COX-2, Nuclear factor-kappaB",
author = "Wang, {Lien Hui} and Huei Lee and Chiang, {Tai An} and Chen, {Mei Ju} and Lin, {Shwu Yuann}",
year = "2011",
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language = "English",
volume = "19",
journal = "Journal of Food and Drug Analysis",
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T1 - Preventive effect of methanol extract from Agrocybe cylindracea (MEAC) mushroom on the cooking oil fumes-induced expressions of cyclooxygenase-2 via suppression of nuclear factor-kappaB (NF-kB) in CL-3 cells

AU - Wang, Lien Hui

AU - Lee, Huei

AU - Chiang, Tai An

AU - Chen, Mei Ju

AU - Lin, Shwu Yuann

PY - 2011/9

Y1 - 2011/9

N2 - Methanol extract from Agrocybe cylindracea strain B (MEAC) mushroom is reported to suppress reactive oxygen species (ROS) production and DNA oxidative damage induced by cooking oil fumes (COF). This study examined the effects of MEAC on COF-stimulated expression of the cyclooxygenase-2 (COX-2) and cytochrome P450 CYP1A1 genes in human lung adenocarcinoma CL-3 cells. MEAC did not alter COF-stimulated CYP1A1 mRNA expression. However, MEAC significantly inhibited COF (100 μg/mL) stimulation of COX-2 mRNA and protein levels in a dose-dependent manner. The concentration of MEAC that decreased COF-stimulated COX-2 protein expression by 50% was 500 μg/mL. These data suggest that MEAC selectively affects particular types of the COF-mediated genes. COX-2 is reported to be regulated by the nuclear factor-kappaB (NF-kB) pathway. The gel retardation assay was used and showed that MEAC alone did not alter the basal NF-kB DNA binding activity; in the presence of COF, it dose-dependently suppressed COF-increased activity of NF-kB. These data suggest that MEAC may exert its anti-COF action on COX-2 gene expression via the NF-kB pathway. The results of this study support that MEAC mediates the COF-stimulated ROS production and DNA damage in CL-3 lung cancer cells.

AB - Methanol extract from Agrocybe cylindracea strain B (MEAC) mushroom is reported to suppress reactive oxygen species (ROS) production and DNA oxidative damage induced by cooking oil fumes (COF). This study examined the effects of MEAC on COF-stimulated expression of the cyclooxygenase-2 (COX-2) and cytochrome P450 CYP1A1 genes in human lung adenocarcinoma CL-3 cells. MEAC did not alter COF-stimulated CYP1A1 mRNA expression. However, MEAC significantly inhibited COF (100 μg/mL) stimulation of COX-2 mRNA and protein levels in a dose-dependent manner. The concentration of MEAC that decreased COF-stimulated COX-2 protein expression by 50% was 500 μg/mL. These data suggest that MEAC selectively affects particular types of the COF-mediated genes. COX-2 is reported to be regulated by the nuclear factor-kappaB (NF-kB) pathway. The gel retardation assay was used and showed that MEAC alone did not alter the basal NF-kB DNA binding activity; in the presence of COF, it dose-dependently suppressed COF-increased activity of NF-kB. These data suggest that MEAC may exert its anti-COF action on COX-2 gene expression via the NF-kB pathway. The results of this study support that MEAC mediates the COF-stimulated ROS production and DNA damage in CL-3 lung cancer cells.

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