Abstract

The three purgative Cheng-Chi-Tang decoctions (CCTDs) including Ta-Cheng-Chi-Tang (TCCT), Xiao-Chen-Chi-Tang (XCCT), and Tiao-Wei-Chen-Chi-Tang (TWCCT) are used for treating gastrointestinal disorders, including liver diseases in traditional Chinese medicine. However, the underlying mechanisms as liver disease remedies are far from fully clarified. The objective of the study is to investigate and compare the antioxidant activity of the three purgative CCTDs in order to delineate their hepatic protective potential and mechanism. Antioxidant activity measured with the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging test indicated XCCT as the most potent preparation (IC50 8.94 μg/ml). In tert-butylhydroperoxide (TBH, 50 mM)-induced lipid peroxidation in ICR mice liver homogenates, XCCT also showed stronger and dose-dependent inhibitory activity against TBH-induced malondialdehyde (MDA, a marker of lipid peroxidation) production (IC50 53.66 μg/ml). In addition, XCCT showed dose-dependent protective effect against TBH-induced cytotoxicity in normal human Chung liver cells Furthermore, in carbon tetrachloride (CCl4)-induced acute liver injury model, mice pretreated with 0.2 g/kg and 0.4 g/kg of XCCT extracts showed a decrease of 59.8 and 43.1% in serum glutamic oxaloactetic transaminase (GOT) level, 51.4 and 52% in glutamic pyruvate transaminase (GPT) level, along with a reduction of 31 and 15% in MDA level, respectively, similar to the effects exerted by silymarin. XCCT pretreated mice also showed milder necrotic changes in the microscopic picture of the liver. The results suggest that XCCT has significant antioxidant activity and hepatic protection potential.

Original languageEnglish
Pages (from-to)232-239
Number of pages8
JournalJournal of Ethnopharmacology
Volume111
Issue number2
DOIs
Publication statusPublished - May 4 2007

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Liver
Wounds and Injuries
Cathartics
Antioxidants
Transaminases
Lipid Peroxidation
Inhibitory Concentration 50
Liver Diseases
Silymarin
tert-Butylhydroperoxide
Inbred ICR Mouse
Carbon Tetrachloride
Chinese Traditional Medicine
Malondialdehyde
Pyruvic Acid
Serum

Keywords

  • Liver disease
  • Oxidative injury
  • Polygonaceae
  • Purgative decoctions
  • Rheum palmatum
  • Xiao-Chen-Chi-Tang

ASJC Scopus subject areas

  • Pharmacology

Cite this

Prevention of hepatic oxidative injury by Xiao-Chen-Chi-Tang in mice. / Tseng, Sung Hui; Chien, Ting Yi; Tzeng, Chih Fu; Lin, Yun Ho; Wu, Chih Hsiung; Wang, Ching Chiung.

In: Journal of Ethnopharmacology, Vol. 111, No. 2, 04.05.2007, p. 232-239.

Research output: Contribution to journalArticle

Tseng, Sung Hui ; Chien, Ting Yi ; Tzeng, Chih Fu ; Lin, Yun Ho ; Wu, Chih Hsiung ; Wang, Ching Chiung. / Prevention of hepatic oxidative injury by Xiao-Chen-Chi-Tang in mice. In: Journal of Ethnopharmacology. 2007 ; Vol. 111, No. 2. pp. 232-239.
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AU - Wang, Ching Chiung

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