Prevention of cellular oxidative damage by an aqueous extract of Anoectochilus formosanus

Leng-Fang Wang, Chun Mao Lin, Chwen Ming Shih, Hui Ju Chen, Borcherng Su, Cheng Chuang Tseng, Bao Bih Gau, Kur Ta Cheng

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Anoectochilus formosanus (AF) is a popular folk medicine in Taiwan whose pharmacological effects have been characterized. In this work we investigated the antioxidant properties of an aqueous extract prepared from AF. The AF extract was capable of scavenging H2O2 in a dose-dependent manner. We induced oxidative stress in HL-60 cells, either by the addition of hydrogen peroxide (H2O2) or by the xanthine/xanthine oxidase reaction. Apoptosis caused by oxidative damage was displayed by DNA fragmentation on gel electrophoresis, and the apoptotic fraction was quantified with flow cytometry. The cell damage induced by oxidative stress was prevented by the plant extract in a concentration-dependent manner. Furthermore, the proteolytic cleavage of poly(ADP-ribose) polymerase during the apoptotic process was also inhibited by AF extract. Our results provide the basis for determining an AF extract to be an antioxidant.

Original languageEnglish
Pages (from-to)379-386
Number of pages8
JournalAnnals of the New York Academy of Sciences
Volume1042
DOIs
Publication statusPublished - 2005

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Keywords

  • Anoectochilus formosanus
  • Apoptosis
  • PARP
  • Reactive oxygen species

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • History and Philosophy of Science

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