Prevalence and species identification of cryptosporidium from fecal samples of horses in Taiwan

Peir Fen Guo, Tina Tu Wen Chen, John Chin Tsaihong, Gen Der Ho, Po Ching Cheng, Yu Chuan Tseng, Shih Yi Peng

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Cryptosporidiosis is a zoonotic disease caused by the protozoan parasite Cryptosporidium. A total of 436 horse fecal samples were collected from 19 farms, and acid-fast staining method was used for primary screening. Cryptosporidium oocysts were found in 161 samples, among which 33 positive sample were selected for nested PCR, restriction fragment length polymorphism analysis and DNA sequencing of 18 S rDNA, showing 31 samples to be bovine C. parvum and 2 C. felis. The methods employed in this study should be useful as tools to identify cryptosporidiosis genotypes and species of livestock.

Original languageEnglish
Pages (from-to)6-12
Number of pages7
JournalSoutheast Asian Journal of Tropical Medicine and Public Health
Volume45
Issue number1
Publication statusPublished - 2014
Externally publishedYes

Fingerprint

Cryptosporidiosis
Cryptosporidium
Taiwan
Horses
Felis
Oocysts
Zoonoses
Livestock
Ribosomal DNA
DNA Sequence Analysis
Restriction Fragment Length Polymorphisms
Parasites
Genotype
Staining and Labeling
Polymerase Chain Reaction
Acids
Farms

Keywords

  • Cryptosporidium
  • Genotype
  • Horse
  • Nested PCR
  • RFLP
  • Taiwan

ASJC Scopus subject areas

  • Infectious Diseases
  • Public Health, Environmental and Occupational Health

Cite this

Prevalence and species identification of cryptosporidium from fecal samples of horses in Taiwan. / Guo, Peir Fen; Chen, Tina Tu Wen; Tsaihong, John Chin; Ho, Gen Der; Cheng, Po Ching; Tseng, Yu Chuan; Peng, Shih Yi.

In: Southeast Asian Journal of Tropical Medicine and Public Health, Vol. 45, No. 1, 2014, p. 6-12.

Research output: Contribution to journalArticle

Guo, Peir Fen ; Chen, Tina Tu Wen ; Tsaihong, John Chin ; Ho, Gen Der ; Cheng, Po Ching ; Tseng, Yu Chuan ; Peng, Shih Yi. / Prevalence and species identification of cryptosporidium from fecal samples of horses in Taiwan. In: Southeast Asian Journal of Tropical Medicine and Public Health. 2014 ; Vol. 45, No. 1. pp. 6-12.
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