Pretreatment with an ethanolic extract of Taiwanofungus camphoratus (Antrodia camphorata) enhances the cytotoxic effects of amphotericin B

Ling Yi Chen, Ming Thau Sheu, Der Zen Liu, Chuh Kai Liao, Hsiu O. Ho, Woei Yao Kao, Yaun Soon Ho, Wen Sen Lee, Ching-Hua Su

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15 Citations (Scopus)

Abstract

Taiwanofungus camphoratus, a well-known Chinese medicine used in Taiwan, possesses several pharmacological functions, including anticancer effects. In the present study, we aimed to investigate a novel anticancer effect by pretreating cancer cells with an ethanolic extract of T. camphoratus (TCEE) followed by the administration of an antifungal agent amphotericin B (AmB). Both TCEE and AmB showed significant dose-dependent cytotoxicity in HT29 cells. Pretreatment with a nontoxic dose of TCEE enhanced the cytotoxicity of AmB. Furthermore, significant apoptotic cell death was found in cells treated with TCEE and AmB. A combination treatment with AmB plus TCEE resulted in a significant repression of tumor growth in HT29 xenografts. Collectively, our results indicated that combined treatment with AmB and TCEE effectively induced apoptosis and inhibited tumor growth. In the future, TCEE may serve as a potential complementary and alternative medicine to treat patients suffering from colorectal cancer.

Original languageEnglish
Pages (from-to)11255-11263
Number of pages9
JournalJournal of Agricultural and Food Chemistry
Volume59
Issue number20
DOIs
Publication statusPublished - Oct 26 2011

Fingerprint

Antrodia
Taiwanofungus camphoratus
amphotericin B
Amphotericin B
cytotoxicity
pretreatment
extracts
alternative medicine
Cytotoxicity
Complementary Therapies
Medicine
Tumors
Neoplasms
HT29 Cells
neoplasms
antifungal agents
Antifungal Agents
Bioelectric potentials
Cell death
Growth

Keywords

  • amphotericin B
  • antifungal agents
  • Antrodia camphorata
  • apoptosis
  • colon cancer
  • Taiwanofungus camphoratus

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Chemistry(all)

Cite this

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title = "Pretreatment with an ethanolic extract of Taiwanofungus camphoratus (Antrodia camphorata) enhances the cytotoxic effects of amphotericin B",
abstract = "Taiwanofungus camphoratus, a well-known Chinese medicine used in Taiwan, possesses several pharmacological functions, including anticancer effects. In the present study, we aimed to investigate a novel anticancer effect by pretreating cancer cells with an ethanolic extract of T. camphoratus (TCEE) followed by the administration of an antifungal agent amphotericin B (AmB). Both TCEE and AmB showed significant dose-dependent cytotoxicity in HT29 cells. Pretreatment with a nontoxic dose of TCEE enhanced the cytotoxicity of AmB. Furthermore, significant apoptotic cell death was found in cells treated with TCEE and AmB. A combination treatment with AmB plus TCEE resulted in a significant repression of tumor growth in HT29 xenografts. Collectively, our results indicated that combined treatment with AmB and TCEE effectively induced apoptosis and inhibited tumor growth. In the future, TCEE may serve as a potential complementary and alternative medicine to treat patients suffering from colorectal cancer.",
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author = "Chen, {Ling Yi} and Sheu, {Ming Thau} and Liu, {Der Zen} and Liao, {Chuh Kai} and Ho, {Hsiu O.} and Kao, {Woei Yao} and Ho, {Yaun Soon} and Lee, {Wen Sen} and Ching-Hua Su",
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T1 - Pretreatment with an ethanolic extract of Taiwanofungus camphoratus (Antrodia camphorata) enhances the cytotoxic effects of amphotericin B

AU - Chen, Ling Yi

AU - Sheu, Ming Thau

AU - Liu, Der Zen

AU - Liao, Chuh Kai

AU - Ho, Hsiu O.

AU - Kao, Woei Yao

AU - Ho, Yaun Soon

AU - Lee, Wen Sen

AU - Su, Ching-Hua

PY - 2011/10/26

Y1 - 2011/10/26

N2 - Taiwanofungus camphoratus, a well-known Chinese medicine used in Taiwan, possesses several pharmacological functions, including anticancer effects. In the present study, we aimed to investigate a novel anticancer effect by pretreating cancer cells with an ethanolic extract of T. camphoratus (TCEE) followed by the administration of an antifungal agent amphotericin B (AmB). Both TCEE and AmB showed significant dose-dependent cytotoxicity in HT29 cells. Pretreatment with a nontoxic dose of TCEE enhanced the cytotoxicity of AmB. Furthermore, significant apoptotic cell death was found in cells treated with TCEE and AmB. A combination treatment with AmB plus TCEE resulted in a significant repression of tumor growth in HT29 xenografts. Collectively, our results indicated that combined treatment with AmB and TCEE effectively induced apoptosis and inhibited tumor growth. In the future, TCEE may serve as a potential complementary and alternative medicine to treat patients suffering from colorectal cancer.

AB - Taiwanofungus camphoratus, a well-known Chinese medicine used in Taiwan, possesses several pharmacological functions, including anticancer effects. In the present study, we aimed to investigate a novel anticancer effect by pretreating cancer cells with an ethanolic extract of T. camphoratus (TCEE) followed by the administration of an antifungal agent amphotericin B (AmB). Both TCEE and AmB showed significant dose-dependent cytotoxicity in HT29 cells. Pretreatment with a nontoxic dose of TCEE enhanced the cytotoxicity of AmB. Furthermore, significant apoptotic cell death was found in cells treated with TCEE and AmB. A combination treatment with AmB plus TCEE resulted in a significant repression of tumor growth in HT29 xenografts. Collectively, our results indicated that combined treatment with AmB and TCEE effectively induced apoptosis and inhibited tumor growth. In the future, TCEE may serve as a potential complementary and alternative medicine to treat patients suffering from colorectal cancer.

KW - amphotericin B

KW - antifungal agents

KW - Antrodia camphorata

KW - apoptosis

KW - colon cancer

KW - Taiwanofungus camphoratus

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