Presumptive identification of streptococci by pyrrolidonyl-beta- naphthylamide (PYR) test

Chi Hsiang Chen, Li Ung Huang, Janq Hwa Lee, Wei Hwa Lee

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Background. Group A streptococci and enterococci can be differentiated from other streptococci on the basis of their ability to cleave L- pyrrolidonyl-beta-naphthylamide. Methods. In the present study, the L- pyrrolidonyl-beta-naphthylamide (PYR) test, pigment medium and bile esculin medium have been used to presumptively identify the streptococci. In total, 114 strains of group A streptococci, 350 strains of non-group A streptococci, 202 strains of enterococci and 197 strains of non-enterococci have been tested. Results. The results of the present investigation show that sensitivities of different test methods are: PYR broth, 99.08%; Murex PYR, 98.48%; bacitracin, 95%; bacitracin and sulfamethoxazole/trimethoprim (SXT), 95%; pigment medium, 99.23%; bile esculin medium, 99.26%. Additionally, specificities of various tests are: PYR broth and Murex PYR, 99.82%; bacitracin, 90.90%; bacitracin and SXT, 98.87%; pigment medium and bile esculin medium, 100%, respectively. Conclusions. PYR test has been observed to be very easy to use and may hence be considered as a rapid, reliable and cost-effective method for presumptive identification of group A streptococci and enterococci in the clinical laboratory.

Original languageEnglish
Pages (from-to)259-264
Number of pages6
JournalChinese Medical Journal (Taipei)
Volume59
Issue number4
Publication statusPublished - 1997
Externally publishedYes

Fingerprint

Streptococcus
Bacitracin
Esculin
Enterococcus
Bile Pigments
Sulfamethoxazole Drug Combination Trimethoprim
Social Identification
Aptitude
pyrrolidonyl-beta-naphthylamide
Bile
Costs and Cost Analysis

Keywords

  • Enterococci
  • Group A streptococci
  • PYR test

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Presumptive identification of streptococci by pyrrolidonyl-beta- naphthylamide (PYR) test. / Chen, Chi Hsiang; Huang, Li Ung; Lee, Janq Hwa; Lee, Wei Hwa.

In: Chinese Medical Journal (Taipei), Vol. 59, No. 4, 1997, p. 259-264.

Research output: Contribution to journalArticle

Chen, Chi Hsiang ; Huang, Li Ung ; Lee, Janq Hwa ; Lee, Wei Hwa. / Presumptive identification of streptococci by pyrrolidonyl-beta- naphthylamide (PYR) test. In: Chinese Medical Journal (Taipei). 1997 ; Vol. 59, No. 4. pp. 259-264.
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