Presence of secretory cellular apoptosis susceptibility protein in cerebrospinal fluids of patients with intracerebral hemorrhage caused by stroke and neurotrauma

Jai Nien Tung, Tang Yi Tsao, Shun Liang Chen, Cheng Jeng Tai, Shing Chuan Shen, Ya Wen Cheng, Ming Chung Jiang

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

OBJECTIVE: The blood-brain barrier (BBB) is a specialized structure that separates blood vessels from the central nervous system (CNS) and restricts the entry of biomolecules and cells into the brain. Matrix metalloproteinase-2 (MMP-2) produced by interferon-γ-activated microglia (brain macrophages) is essential for disrupting the glia limitans of BBB, which is critical for lymphocytes penetration into brain capillaries in various CNS disorders. The cellular apoptosis susceptibility (CSE1L/CAS) protein has been shown to regulate MMP-2 secretion. METHODS: We examined if CSE1L played a role in regulating the progression of intracerebral brain hemorrhage disorders. RESULTS: CSE1L was detected by immunoblotting in cerebrospinal fluids (CSFs) of patients with intracerebral hemorrhage brain disorders, including stroke and neurotrauma. Interferon-γ treatment induced CSE1L expression and increased the secretions of CSE1L and MMP-2 by U937 macrophages. Moreover, tranfection of U937 macrophages with siRNA that targeted CSE1L inhibited interferon-γ-induced CSE1L and MMP-2 secretion by U937 macrophages. The numbers of lymphocytes in CSF were correlated with the levels of CSE1L and MMP-2 in patients' CSF. CONCLUSIONS: Our results suggest that CSE1L plays a role in regulating MMP-2-mediated BBB breakdown and it may be a target for control of BBB permeability in intracerebral brain hemorrhage disorders.

Original languageEnglish
Pages (from-to)390-398
Number of pages9
JournalNeuroendocrinology Letters
Volume31
Issue number3
Publication statusPublished - 2010

Fingerprint

Cellular Apoptosis Susceptibility Protein
Matrix Metalloproteinase 2
Cerebral Hemorrhage
Cerebrospinal Fluid
Stroke
Blood-Brain Barrier
Brain Diseases
Macrophages
Interferons
Intracranial Hemorrhages
Brain
Central Nervous System Diseases
Lymphocyte Count
Microglia
Immunoblotting
Neuroglia
Small Interfering RNA
Blood Vessels
Permeability
Central Nervous System

Keywords

  • Blood-brain barrier
  • Cerebrospinal fluids
  • Interferon-γ
  • Matrix metalloproteinase-2
  • Microglia

ASJC Scopus subject areas

  • Endocrinology
  • Endocrine and Autonomic Systems
  • Endocrinology, Diabetes and Metabolism
  • Clinical Neurology
  • Psychiatry and Mental health
  • Neurology
  • Medicine(all)

Cite this

Presence of secretory cellular apoptosis susceptibility protein in cerebrospinal fluids of patients with intracerebral hemorrhage caused by stroke and neurotrauma. / Tung, Jai Nien; Tsao, Tang Yi; Chen, Shun Liang; Tai, Cheng Jeng; Shen, Shing Chuan; Cheng, Ya Wen; Jiang, Ming Chung.

In: Neuroendocrinology Letters, Vol. 31, No. 3, 2010, p. 390-398.

Research output: Contribution to journalArticle

@article{b3e159911e3342da8293e927286a2ebd,
title = "Presence of secretory cellular apoptosis susceptibility protein in cerebrospinal fluids of patients with intracerebral hemorrhage caused by stroke and neurotrauma",
abstract = "OBJECTIVE: The blood-brain barrier (BBB) is a specialized structure that separates blood vessels from the central nervous system (CNS) and restricts the entry of biomolecules and cells into the brain. Matrix metalloproteinase-2 (MMP-2) produced by interferon-γ-activated microglia (brain macrophages) is essential for disrupting the glia limitans of BBB, which is critical for lymphocytes penetration into brain capillaries in various CNS disorders. The cellular apoptosis susceptibility (CSE1L/CAS) protein has been shown to regulate MMP-2 secretion. METHODS: We examined if CSE1L played a role in regulating the progression of intracerebral brain hemorrhage disorders. RESULTS: CSE1L was detected by immunoblotting in cerebrospinal fluids (CSFs) of patients with intracerebral hemorrhage brain disorders, including stroke and neurotrauma. Interferon-γ treatment induced CSE1L expression and increased the secretions of CSE1L and MMP-2 by U937 macrophages. Moreover, tranfection of U937 macrophages with siRNA that targeted CSE1L inhibited interferon-γ-induced CSE1L and MMP-2 secretion by U937 macrophages. The numbers of lymphocytes in CSF were correlated with the levels of CSE1L and MMP-2 in patients' CSF. CONCLUSIONS: Our results suggest that CSE1L plays a role in regulating MMP-2-mediated BBB breakdown and it may be a target for control of BBB permeability in intracerebral brain hemorrhage disorders.",
keywords = "Blood-brain barrier, Cerebrospinal fluids, Interferon-γ, Matrix metalloproteinase-2, Microglia",
author = "Tung, {Jai Nien} and Tsao, {Tang Yi} and Chen, {Shun Liang} and Tai, {Cheng Jeng} and Shen, {Shing Chuan} and Cheng, {Ya Wen} and Jiang, {Ming Chung}",
year = "2010",
language = "English",
volume = "31",
pages = "390--398",
journal = "Neuroendocrinology Letters",
issn = "0172-780X",
publisher = "Maghira and Maas Publications",
number = "3",

}

TY - JOUR

T1 - Presence of secretory cellular apoptosis susceptibility protein in cerebrospinal fluids of patients with intracerebral hemorrhage caused by stroke and neurotrauma

AU - Tung, Jai Nien

AU - Tsao, Tang Yi

AU - Chen, Shun Liang

AU - Tai, Cheng Jeng

AU - Shen, Shing Chuan

AU - Cheng, Ya Wen

AU - Jiang, Ming Chung

PY - 2010

Y1 - 2010

N2 - OBJECTIVE: The blood-brain barrier (BBB) is a specialized structure that separates blood vessels from the central nervous system (CNS) and restricts the entry of biomolecules and cells into the brain. Matrix metalloproteinase-2 (MMP-2) produced by interferon-γ-activated microglia (brain macrophages) is essential for disrupting the glia limitans of BBB, which is critical for lymphocytes penetration into brain capillaries in various CNS disorders. The cellular apoptosis susceptibility (CSE1L/CAS) protein has been shown to regulate MMP-2 secretion. METHODS: We examined if CSE1L played a role in regulating the progression of intracerebral brain hemorrhage disorders. RESULTS: CSE1L was detected by immunoblotting in cerebrospinal fluids (CSFs) of patients with intracerebral hemorrhage brain disorders, including stroke and neurotrauma. Interferon-γ treatment induced CSE1L expression and increased the secretions of CSE1L and MMP-2 by U937 macrophages. Moreover, tranfection of U937 macrophages with siRNA that targeted CSE1L inhibited interferon-γ-induced CSE1L and MMP-2 secretion by U937 macrophages. The numbers of lymphocytes in CSF were correlated with the levels of CSE1L and MMP-2 in patients' CSF. CONCLUSIONS: Our results suggest that CSE1L plays a role in regulating MMP-2-mediated BBB breakdown and it may be a target for control of BBB permeability in intracerebral brain hemorrhage disorders.

AB - OBJECTIVE: The blood-brain barrier (BBB) is a specialized structure that separates blood vessels from the central nervous system (CNS) and restricts the entry of biomolecules and cells into the brain. Matrix metalloproteinase-2 (MMP-2) produced by interferon-γ-activated microglia (brain macrophages) is essential for disrupting the glia limitans of BBB, which is critical for lymphocytes penetration into brain capillaries in various CNS disorders. The cellular apoptosis susceptibility (CSE1L/CAS) protein has been shown to regulate MMP-2 secretion. METHODS: We examined if CSE1L played a role in regulating the progression of intracerebral brain hemorrhage disorders. RESULTS: CSE1L was detected by immunoblotting in cerebrospinal fluids (CSFs) of patients with intracerebral hemorrhage brain disorders, including stroke and neurotrauma. Interferon-γ treatment induced CSE1L expression and increased the secretions of CSE1L and MMP-2 by U937 macrophages. Moreover, tranfection of U937 macrophages with siRNA that targeted CSE1L inhibited interferon-γ-induced CSE1L and MMP-2 secretion by U937 macrophages. The numbers of lymphocytes in CSF were correlated with the levels of CSE1L and MMP-2 in patients' CSF. CONCLUSIONS: Our results suggest that CSE1L plays a role in regulating MMP-2-mediated BBB breakdown and it may be a target for control of BBB permeability in intracerebral brain hemorrhage disorders.

KW - Blood-brain barrier

KW - Cerebrospinal fluids

KW - Interferon-γ

KW - Matrix metalloproteinase-2

KW - Microglia

UR - http://www.scopus.com/inward/record.url?scp=77955455284&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77955455284&partnerID=8YFLogxK

M3 - Article

C2 - 20588233

AN - SCOPUS:77955455284

VL - 31

SP - 390

EP - 398

JO - Neuroendocrinology Letters

JF - Neuroendocrinology Letters

SN - 0172-780X

IS - 3

ER -