Abstract
Vitis thunbergii var. taiwaniana is a vitaceous native folk medicinal plant in Taiwan. The root of V. thunbergii var. taiwaniana is used to treat hepatitis and arthritis. Centrifugal partition chromatography (CPC) and Sephadex LH-20 gel filtration column chromatography were successively used to isolate and purify three oligostilbenes, (-)-vitisin B, (+)-vitisin A, and ampelopsin A, from the root of V. thunbergii var. taiwaniana. The two-phase solvent system of CPC composed of chloroform-methanol-water (6:7:4, v/v/v) was used for the preparative separation of the methanolic extract of V. thunbergii var. taiwaniana. Using CPC and Sephadex LH-20 column chromatography, about 1 g of the crude extract was separated, yielding 28.9 mg of (-)-vitisin B, 18.7 mg of (+)-vitisin A, and 18.2 mg of ampelopsin A at respective purities of 93%, 91%, and 90%.
| Original language | English |
|---|---|
| Pages (from-to) | 65-70 |
| Number of pages | 6 |
| Journal | Separation and Purification Technology |
| Volume | 66 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Apr 7 2009 |
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Keywords
- (-)-Vitisin B
- Centrifugal partition chromatography
- Oligostilbene
- Sephadex LH-20
- Vitis thunbergii var. taiwaniana
ASJC Scopus subject areas
- Filtration and Separation
- Analytical Chemistry
Cite this
Preparative separation of oligostilbenes from Vitis thunbergii var. taiwaniana by centrifugal partition chromatography followed by Sephadex LH-20 column chromatography. / Chen, Lih Geeng; Wang, Ching Chiung.
In: Separation and Purification Technology, Vol. 66, No. 1, 07.04.2009, p. 65-70.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Preparative separation of oligostilbenes from Vitis thunbergii var. taiwaniana by centrifugal partition chromatography followed by Sephadex LH-20 column chromatography
AU - Chen, Lih Geeng
AU - Wang, Ching Chiung
PY - 2009/4/7
Y1 - 2009/4/7
N2 - Vitis thunbergii var. taiwaniana is a vitaceous native folk medicinal plant in Taiwan. The root of V. thunbergii var. taiwaniana is used to treat hepatitis and arthritis. Centrifugal partition chromatography (CPC) and Sephadex LH-20 gel filtration column chromatography were successively used to isolate and purify three oligostilbenes, (-)-vitisin B, (+)-vitisin A, and ampelopsin A, from the root of V. thunbergii var. taiwaniana. The two-phase solvent system of CPC composed of chloroform-methanol-water (6:7:4, v/v/v) was used for the preparative separation of the methanolic extract of V. thunbergii var. taiwaniana. Using CPC and Sephadex LH-20 column chromatography, about 1 g of the crude extract was separated, yielding 28.9 mg of (-)-vitisin B, 18.7 mg of (+)-vitisin A, and 18.2 mg of ampelopsin A at respective purities of 93%, 91%, and 90%.
AB - Vitis thunbergii var. taiwaniana is a vitaceous native folk medicinal plant in Taiwan. The root of V. thunbergii var. taiwaniana is used to treat hepatitis and arthritis. Centrifugal partition chromatography (CPC) and Sephadex LH-20 gel filtration column chromatography were successively used to isolate and purify three oligostilbenes, (-)-vitisin B, (+)-vitisin A, and ampelopsin A, from the root of V. thunbergii var. taiwaniana. The two-phase solvent system of CPC composed of chloroform-methanol-water (6:7:4, v/v/v) was used for the preparative separation of the methanolic extract of V. thunbergii var. taiwaniana. Using CPC and Sephadex LH-20 column chromatography, about 1 g of the crude extract was separated, yielding 28.9 mg of (-)-vitisin B, 18.7 mg of (+)-vitisin A, and 18.2 mg of ampelopsin A at respective purities of 93%, 91%, and 90%.
KW - (-)-Vitisin B
KW - Centrifugal partition chromatography
KW - Oligostilbene
KW - Sephadex LH-20
KW - Vitis thunbergii var. taiwaniana
UR - http://www.scopus.com/inward/record.url?scp=62149119591&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=62149119591&partnerID=8YFLogxK
U2 - 10.1016/j.seppur.2008.11.025
DO - 10.1016/j.seppur.2008.11.025
M3 - Article
VL - 66
SP - 65
EP - 70
JO - Separation and Purification Technology
JF - Separation and Purification Technology
SN - 1383-5866
IS - 1
ER -