Preparative separation of oligostilbenes from Vitis thunbergii var. taiwaniana by centrifugal partition chromatography followed by Sephadex LH-20 column chromatography

Lih Geeng Chen, Ching Chiung Wang

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Vitis thunbergii var. taiwaniana is a vitaceous native folk medicinal plant in Taiwan. The root of V. thunbergii var. taiwaniana is used to treat hepatitis and arthritis. Centrifugal partition chromatography (CPC) and Sephadex LH-20 gel filtration column chromatography were successively used to isolate and purify three oligostilbenes, (-)-vitisin B, (+)-vitisin A, and ampelopsin A, from the root of V. thunbergii var. taiwaniana. The two-phase solvent system of CPC composed of chloroform-methanol-water (6:7:4, v/v/v) was used for the preparative separation of the methanolic extract of V. thunbergii var. taiwaniana. Using CPC and Sephadex LH-20 column chromatography, about 1 g of the crude extract was separated, yielding 28.9 mg of (-)-vitisin B, 18.7 mg of (+)-vitisin A, and 18.2 mg of ampelopsin A at respective purities of 93%, 91%, and 90%.

Original languageEnglish
Pages (from-to)65-70
Number of pages6
JournalSeparation and Purification Technology
Volume66
Issue number1
DOIs
Publication statusPublished - Apr 7 2009

Keywords

  • (-)-Vitisin B
  • Centrifugal partition chromatography
  • Oligostilbene
  • Sephadex LH-20
  • Vitis thunbergii var. taiwaniana

ASJC Scopus subject areas

  • Filtration and Separation
  • Analytical Chemistry

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