Abstract
Vitis thunbergii var. taiwaniana is a vitaceous native folk medicinal plant in Taiwan. The root of V. thunbergii var. taiwaniana is used to treat hepatitis and arthritis. Centrifugal partition chromatography (CPC) and Sephadex LH-20 gel filtration column chromatography were successively used to isolate and purify three oligostilbenes, (-)-vitisin B, (+)-vitisin A, and ampelopsin A, from the root of V. thunbergii var. taiwaniana. The two-phase solvent system of CPC composed of chloroform-methanol-water (6:7:4, v/v/v) was used for the preparative separation of the methanolic extract of V. thunbergii var. taiwaniana. Using CPC and Sephadex LH-20 column chromatography, about 1 g of the crude extract was separated, yielding 28.9 mg of (-)-vitisin B, 18.7 mg of (+)-vitisin A, and 18.2 mg of ampelopsin A at respective purities of 93%, 91%, and 90%.
Original language | English |
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Pages (from-to) | 65-70 |
Number of pages | 6 |
Journal | Separation and Purification Technology |
Volume | 66 |
Issue number | 1 |
DOIs | |
Publication status | Published - Apr 7 2009 |
Keywords
- (-)-Vitisin B
- Centrifugal partition chromatography
- Oligostilbene
- Sephadex LH-20
- Vitis thunbergii var. taiwaniana
ASJC Scopus subject areas
- Filtration and Separation
- Analytical Chemistry