Preparation of UHMWPE particles and establishment of inverted macrophage cell model to investigate wear particles induced bioactivites

Hsu Wei Fang, Yi Ching Ho, Charng Bin Yang, Hsuan Liang Liu, Fang Yuan Ho, Yung Chang Lu, Hon Ming Ma, Chun Hsiung Huang

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Total joint replacement surgery has been widely applied to patients with severe osteoarthritis. Aseptic loosening induced by wear particles generated during joint movement is the major reason causing the failure of joint implants. Interaction of ultra-high molecular weight polyethylene (UHMWPE) wear particles with macrophages stimulates the release of inflammatory cytokines and leads to bone resorption and osteolysis. Effect of UHMWPE particle size and shape on the bioactivities remains unclear due to the lack of particles with controlled morphology as well as adequate in-vitro cell culture models for further investigations. We have developed a micro-cutting procedure to generate UHMWPE particles with desired sizes and shapes by rubbing UHMWPE with microfabricated surfaces. A narrow distribution and sterility of the generated particles was achieved. An inverted cell culturing apparatus and procedures were created and the contact between particles and macrophage cells was observed. No significant difference of the cell proliferations under normal and inverted positions further demonstrates the feasibility of the system. This newly developed platform can assist in the further understanding of the mechanism and therapy strategies of osteolysis induced by polyethylene particles.

Original languageEnglish
Pages (from-to)175-187
Number of pages13
JournalJournal of Biochemical and Biophysical Methods
Volume68
Issue number3
DOIs
Publication statusPublished - Oct 31 2006
Externally publishedYes

Fingerprint

Macrophages
Wear of materials
Osteolysis
Replacement Arthroplasties
Joints
Cell proliferation
Polyethylene
Bone Resorption
Bioactivity
Cell culture
Particle Size
Osteoarthritis
Surgery
Infertility
Bone
Cell Culture Techniques
Particle size
Cell Proliferation
Cytokines
ultra-high molecular weight polyethylene

Keywords

  • Cell culture
  • Macrophage
  • Osteolysis
  • Phagocytosis
  • UHMWPE particles

ASJC Scopus subject areas

  • Biophysics

Cite this

Preparation of UHMWPE particles and establishment of inverted macrophage cell model to investigate wear particles induced bioactivites. / Fang, Hsu Wei; Ho, Yi Ching; Yang, Charng Bin; Liu, Hsuan Liang; Ho, Fang Yuan; Lu, Yung Chang; Ma, Hon Ming; Huang, Chun Hsiung.

In: Journal of Biochemical and Biophysical Methods, Vol. 68, No. 3, 31.10.2006, p. 175-187.

Research output: Contribution to journalArticle

Fang, Hsu Wei ; Ho, Yi Ching ; Yang, Charng Bin ; Liu, Hsuan Liang ; Ho, Fang Yuan ; Lu, Yung Chang ; Ma, Hon Ming ; Huang, Chun Hsiung. / Preparation of UHMWPE particles and establishment of inverted macrophage cell model to investigate wear particles induced bioactivites. In: Journal of Biochemical and Biophysical Methods. 2006 ; Vol. 68, No. 3. pp. 175-187.
@article{3b2a2849c799488b91d2265ae6097ec6,
title = "Preparation of UHMWPE particles and establishment of inverted macrophage cell model to investigate wear particles induced bioactivites",
abstract = "Total joint replacement surgery has been widely applied to patients with severe osteoarthritis. Aseptic loosening induced by wear particles generated during joint movement is the major reason causing the failure of joint implants. Interaction of ultra-high molecular weight polyethylene (UHMWPE) wear particles with macrophages stimulates the release of inflammatory cytokines and leads to bone resorption and osteolysis. Effect of UHMWPE particle size and shape on the bioactivities remains unclear due to the lack of particles with controlled morphology as well as adequate in-vitro cell culture models for further investigations. We have developed a micro-cutting procedure to generate UHMWPE particles with desired sizes and shapes by rubbing UHMWPE with microfabricated surfaces. A narrow distribution and sterility of the generated particles was achieved. An inverted cell culturing apparatus and procedures were created and the contact between particles and macrophage cells was observed. No significant difference of the cell proliferations under normal and inverted positions further demonstrates the feasibility of the system. This newly developed platform can assist in the further understanding of the mechanism and therapy strategies of osteolysis induced by polyethylene particles.",
keywords = "Cell culture, Macrophage, Osteolysis, Phagocytosis, UHMWPE particles",
author = "Fang, {Hsu Wei} and Ho, {Yi Ching} and Yang, {Charng Bin} and Liu, {Hsuan Liang} and Ho, {Fang Yuan} and Lu, {Yung Chang} and Ma, {Hon Ming} and Huang, {Chun Hsiung}",
year = "2006",
month = "10",
day = "31",
doi = "10.1016/j.jbbm.2006.05.011",
language = "English",
volume = "68",
pages = "175--187",
journal = "Journal of Proteomics",
issn = "1874-3919",
publisher = "Elsevier",
number = "3",

}

TY - JOUR

T1 - Preparation of UHMWPE particles and establishment of inverted macrophage cell model to investigate wear particles induced bioactivites

AU - Fang, Hsu Wei

AU - Ho, Yi Ching

AU - Yang, Charng Bin

AU - Liu, Hsuan Liang

AU - Ho, Fang Yuan

AU - Lu, Yung Chang

AU - Ma, Hon Ming

AU - Huang, Chun Hsiung

PY - 2006/10/31

Y1 - 2006/10/31

N2 - Total joint replacement surgery has been widely applied to patients with severe osteoarthritis. Aseptic loosening induced by wear particles generated during joint movement is the major reason causing the failure of joint implants. Interaction of ultra-high molecular weight polyethylene (UHMWPE) wear particles with macrophages stimulates the release of inflammatory cytokines and leads to bone resorption and osteolysis. Effect of UHMWPE particle size and shape on the bioactivities remains unclear due to the lack of particles with controlled morphology as well as adequate in-vitro cell culture models for further investigations. We have developed a micro-cutting procedure to generate UHMWPE particles with desired sizes and shapes by rubbing UHMWPE with microfabricated surfaces. A narrow distribution and sterility of the generated particles was achieved. An inverted cell culturing apparatus and procedures were created and the contact between particles and macrophage cells was observed. No significant difference of the cell proliferations under normal and inverted positions further demonstrates the feasibility of the system. This newly developed platform can assist in the further understanding of the mechanism and therapy strategies of osteolysis induced by polyethylene particles.

AB - Total joint replacement surgery has been widely applied to patients with severe osteoarthritis. Aseptic loosening induced by wear particles generated during joint movement is the major reason causing the failure of joint implants. Interaction of ultra-high molecular weight polyethylene (UHMWPE) wear particles with macrophages stimulates the release of inflammatory cytokines and leads to bone resorption and osteolysis. Effect of UHMWPE particle size and shape on the bioactivities remains unclear due to the lack of particles with controlled morphology as well as adequate in-vitro cell culture models for further investigations. We have developed a micro-cutting procedure to generate UHMWPE particles with desired sizes and shapes by rubbing UHMWPE with microfabricated surfaces. A narrow distribution and sterility of the generated particles was achieved. An inverted cell culturing apparatus and procedures were created and the contact between particles and macrophage cells was observed. No significant difference of the cell proliferations under normal and inverted positions further demonstrates the feasibility of the system. This newly developed platform can assist in the further understanding of the mechanism and therapy strategies of osteolysis induced by polyethylene particles.

KW - Cell culture

KW - Macrophage

KW - Osteolysis

KW - Phagocytosis

KW - UHMWPE particles

UR - http://www.scopus.com/inward/record.url?scp=33747874438&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33747874438&partnerID=8YFLogxK

U2 - 10.1016/j.jbbm.2006.05.011

DO - 10.1016/j.jbbm.2006.05.011

M3 - Article

C2 - 16859749

AN - SCOPUS:33747874438

VL - 68

SP - 175

EP - 187

JO - Journal of Proteomics

JF - Journal of Proteomics

SN - 1874-3919

IS - 3

ER -