Preparation of radioiodinated secretin for radioimmtmoassay

Chung-Huei Hsu, Kuo Wei Yin, Tsai Hsia Hong, Tswen Kei Tang, Long Shyong Lee, Tong H. Chang, Jean-Dean Liu, P. H. Chen

Research output: Contribution to journalArticle

Abstract

Radioiodination of synthetic human secretin on its N-terminal histidyl residue was not difficult when a greater amount of Chloramine T and a longer reaction time were employed to achieve better incorporation of125I. The radioiodinated tracer for an optimal radioimmunoassay required purification. The combination of Sep-pak C18 Cartridge and high performance liquid chromatography for the purification of125I-secretin in our study revealed that the Sep-pak cartridge was a preliminary step in removing unlabeled radioactive iodide, the reactant, and labeled materials unadsorbed to the cartridge. The eluate eluted from the Sep-pak containing high radioactivity and high immunoreactivity to the antibody were selected for further purification by HPLC which eliminated undesirable radiolabeled substances with lower immunoreactivity. The purified radiolabeled secretin was used in developing a sensitive radioimmunoassay.

Original languageEnglish
Pages (from-to)83-87
Number of pages5
JournalAnnals of Nuclear Medicine
Volume5
Issue number3
DOIs
Publication statusPublished - Sep 1991
Externally publishedYes

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Keywords

  • HPLC
  • radioimmunoassay
  • radioiodination
  • secretin

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging

Cite this

Hsu, C-H., Yin, K. W., Hong, T. H., Tang, T. K., Lee, L. S., Chang, T. H., Liu, J-D., & Chen, P. H. (1991). Preparation of radioiodinated secretin for radioimmtmoassay. Annals of Nuclear Medicine, 5(3), 83-87. https://doi.org/10.1007/BF03164619