Preparation and properties of pH-responsive, self-assembled colloidal nanoparticles from guanidine-containing polypeptide and chitosan for antibiotic delivery

Yu Ru Su, Shu Huei Yu, An Chong Chao, Jui Yu Wu, Yu Fan Lin, Kun Ying Lu, Fwu Long Mi

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Amoxicillin is a traditional antibiotic used to treat Helicobacter pylori (H. pylori). However, the clinical applicability was limited by low local concentrations of amoxicillin that are reached at the sites of H. pylori infection. In this study, a pH-sensitive, guanidine-containing polypeptide composed of poly(γ-glutamic acid) (γ-PGA) and arginine (Arg) were synthesized. The γ-PGA-g-Arg polypeptide can self-assemble into colloidal nanoparticles at pH lower than 3.0, and the morphological changes are reversibly switched by elevating the pH of the colloidal suspension. The chemo-physical properties of the γ-PGA-g-Arg polypeptide were investigated by proton nuclear magnetic resonance (1H NMR), X-ray diffraction (XRD), and Fourier transform infrared (FTIR) spectroscopy. The γ-PGA-g-Arg colloidal nanoparticles were modified with a guanidinylated polymer, the chitosan (CS)-arginine(Ag) conjugate (CS-N-Arg). The effect of electrostatic complexation between γ-PGA-g-Arg polypeptide and CS-N-Arg conjugate extends the stable range of the self-assembled nanoparticles to a higher pH (pH>6.0), and the surface charge density changes from negative to positive. The morphological changes of the CS-N-Arg/γ-PGA-g-Arg complex nanoparticles in response to environmental pH were investigated by dynamic light scattering (DLS) and transmission electron microscopy (TEM). Amoxicillin release from the CS-N-Arg/γ-PGA-g-Arg NPs was reduced at pH 2.5 (gastric fluid, fasted state) and 4.5 (the gastric mucosal surface), but the antibiotic released rapidly from the nanoparticles at pH 7.0 (the sites of H. pylori infection). The amoxicillin-loaded CS-N-Arg/γ-PGA-g-Arg complex nanoparticles showed a superior antibacterial activity against the growth of H. pylori.

Original languageEnglish
Pages (from-to)9-20
Number of pages12
JournalColloids and Surfaces A: Physicochemical and Engineering Aspects
Volume494
DOIs
Publication statusPublished - Apr 5 2016

Fingerprint

guanidines
Arginine
antibiotics
Polypeptides
Guanidine
polypeptides
Chitosan
Antibiotics
delivery
Prostaglandins A
Anti-Bacterial Agents
Nanoparticles
nanoparticles
Peptides
preparation
Amoxicillin
infectious diseases
Nuclear magnetic resonance
nuclear magnetic resonance
glutamic acid

Keywords

  • Chitosan
  • Colloidal nanoparticles
  • Drug delivery
  • PH-sensitive
  • Polypeptide

ASJC Scopus subject areas

  • Colloid and Surface Chemistry

Cite this

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title = "Preparation and properties of pH-responsive, self-assembled colloidal nanoparticles from guanidine-containing polypeptide and chitosan for antibiotic delivery",
abstract = "Amoxicillin is a traditional antibiotic used to treat Helicobacter pylori (H. pylori). However, the clinical applicability was limited by low local concentrations of amoxicillin that are reached at the sites of H. pylori infection. In this study, a pH-sensitive, guanidine-containing polypeptide composed of poly(γ-glutamic acid) (γ-PGA) and arginine (Arg) were synthesized. The γ-PGA-g-Arg polypeptide can self-assemble into colloidal nanoparticles at pH lower than 3.0, and the morphological changes are reversibly switched by elevating the pH of the colloidal suspension. The chemo-physical properties of the γ-PGA-g-Arg polypeptide were investigated by proton nuclear magnetic resonance (1H NMR), X-ray diffraction (XRD), and Fourier transform infrared (FTIR) spectroscopy. The γ-PGA-g-Arg colloidal nanoparticles were modified with a guanidinylated polymer, the chitosan (CS)-arginine(Ag) conjugate (CS-N-Arg). The effect of electrostatic complexation between γ-PGA-g-Arg polypeptide and CS-N-Arg conjugate extends the stable range of the self-assembled nanoparticles to a higher pH (pH>6.0), and the surface charge density changes from negative to positive. The morphological changes of the CS-N-Arg/γ-PGA-g-Arg complex nanoparticles in response to environmental pH were investigated by dynamic light scattering (DLS) and transmission electron microscopy (TEM). Amoxicillin release from the CS-N-Arg/γ-PGA-g-Arg NPs was reduced at pH 2.5 (gastric fluid, fasted state) and 4.5 (the gastric mucosal surface), but the antibiotic released rapidly from the nanoparticles at pH 7.0 (the sites of H. pylori infection). The amoxicillin-loaded CS-N-Arg/γ-PGA-g-Arg complex nanoparticles showed a superior antibacterial activity against the growth of H. pylori.",
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T1 - Preparation and properties of pH-responsive, self-assembled colloidal nanoparticles from guanidine-containing polypeptide and chitosan for antibiotic delivery

AU - Su, Yu Ru

AU - Yu, Shu Huei

AU - Chao, An Chong

AU - Wu, Jui Yu

AU - Lin, Yu Fan

AU - Lu, Kun Ying

AU - Mi, Fwu Long

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N2 - Amoxicillin is a traditional antibiotic used to treat Helicobacter pylori (H. pylori). However, the clinical applicability was limited by low local concentrations of amoxicillin that are reached at the sites of H. pylori infection. In this study, a pH-sensitive, guanidine-containing polypeptide composed of poly(γ-glutamic acid) (γ-PGA) and arginine (Arg) were synthesized. The γ-PGA-g-Arg polypeptide can self-assemble into colloidal nanoparticles at pH lower than 3.0, and the morphological changes are reversibly switched by elevating the pH of the colloidal suspension. The chemo-physical properties of the γ-PGA-g-Arg polypeptide were investigated by proton nuclear magnetic resonance (1H NMR), X-ray diffraction (XRD), and Fourier transform infrared (FTIR) spectroscopy. The γ-PGA-g-Arg colloidal nanoparticles were modified with a guanidinylated polymer, the chitosan (CS)-arginine(Ag) conjugate (CS-N-Arg). The effect of electrostatic complexation between γ-PGA-g-Arg polypeptide and CS-N-Arg conjugate extends the stable range of the self-assembled nanoparticles to a higher pH (pH>6.0), and the surface charge density changes from negative to positive. The morphological changes of the CS-N-Arg/γ-PGA-g-Arg complex nanoparticles in response to environmental pH were investigated by dynamic light scattering (DLS) and transmission electron microscopy (TEM). Amoxicillin release from the CS-N-Arg/γ-PGA-g-Arg NPs was reduced at pH 2.5 (gastric fluid, fasted state) and 4.5 (the gastric mucosal surface), but the antibiotic released rapidly from the nanoparticles at pH 7.0 (the sites of H. pylori infection). The amoxicillin-loaded CS-N-Arg/γ-PGA-g-Arg complex nanoparticles showed a superior antibacterial activity against the growth of H. pylori.

AB - Amoxicillin is a traditional antibiotic used to treat Helicobacter pylori (H. pylori). However, the clinical applicability was limited by low local concentrations of amoxicillin that are reached at the sites of H. pylori infection. In this study, a pH-sensitive, guanidine-containing polypeptide composed of poly(γ-glutamic acid) (γ-PGA) and arginine (Arg) were synthesized. The γ-PGA-g-Arg polypeptide can self-assemble into colloidal nanoparticles at pH lower than 3.0, and the morphological changes are reversibly switched by elevating the pH of the colloidal suspension. The chemo-physical properties of the γ-PGA-g-Arg polypeptide were investigated by proton nuclear magnetic resonance (1H NMR), X-ray diffraction (XRD), and Fourier transform infrared (FTIR) spectroscopy. The γ-PGA-g-Arg colloidal nanoparticles were modified with a guanidinylated polymer, the chitosan (CS)-arginine(Ag) conjugate (CS-N-Arg). The effect of electrostatic complexation between γ-PGA-g-Arg polypeptide and CS-N-Arg conjugate extends the stable range of the self-assembled nanoparticles to a higher pH (pH>6.0), and the surface charge density changes from negative to positive. The morphological changes of the CS-N-Arg/γ-PGA-g-Arg complex nanoparticles in response to environmental pH were investigated by dynamic light scattering (DLS) and transmission electron microscopy (TEM). Amoxicillin release from the CS-N-Arg/γ-PGA-g-Arg NPs was reduced at pH 2.5 (gastric fluid, fasted state) and 4.5 (the gastric mucosal surface), but the antibiotic released rapidly from the nanoparticles at pH 7.0 (the sites of H. pylori infection). The amoxicillin-loaded CS-N-Arg/γ-PGA-g-Arg complex nanoparticles showed a superior antibacterial activity against the growth of H. pylori.

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