Preparation and characterization of chemically TEMPO-oxidized and mechanically disintegrated sacchachitin nanofibers (SCNF) for enhanced diabetic wound healing

Fang Ching Chao, Meng Huang Wu, Ling Chun Chen, Hong Liang Lin, Der Zen Liu, Hsiu O. Ho, Ming Thau Sheu

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

TEMPO-oxidization and mechanical disintegration were utilized to develop sacchachitin nanofibers (SCNF) with a 3D gel structure for being an ideal scaffold. Mechanically disintegrated SCNF (MDSCNF) with NanoLyzer® at 20,000 psi for 5 cycles and TEMPO-oxidized SCNF (TOSCNF) produced with 5.0 and 10.0 mmole NaClO/g SC was designated as SCN5, T050SC, and T100SC, respectively. All 2% MDSCNF suspensions were demonstrated to be in gel form, while all except T100SC of 2% TOSCNF suspensions showed to be wet fiber-like hydrogel. In diabetic wound healing study, both SCN5 and T050SC incorporated in AMPS (2-acrylamide-2-methyl-propane sulfonate)-based wound dressing were showed to accelerate diabetic wound healing forming nearly the same as normal tissues. T050SC/H further provided the healed wound with growth of sweat glands and hair follicles indicating the wound had healed as functional tissue. Conclusively, TEMPO-oxidized SCNF-based hydrogel scaffolds showed greater potentials in tissue regeneration due to its unique physical and chemical properties.

Original languageEnglish
Article number115507
Pages (from-to)115507
JournalCarbohydrate Polymers
Volume229
DOIs
Publication statusPublished - Feb 1 2020

Keywords

  • 2,2,6,6-tetramethyl-1-piperidinyloxy (TEMPO)
  • 2-acrylamido-2-methyl-1-propanesulfonic acid sodium salt (AMPS)
  • Chitosan
  • Diabetic wound healing
  • Irgacure® 184
  • L-ascorbic acid, poly(ethylene glycol) diacrylate
  • Mechanical disintegration
  • N,N-dimethylacrylamide
  • Nanofibers
  • PEG1000
  • PVP K90
  • Sacchachitin
  • Sodium bromide
  • Sodium hypochlorite
  • TEMPO-oxidation
  • Thiazolyl blue tetrazolium bromide (MTT reagent)
  • chitosan
  • sodium bromide
  • thiazolyl blue tetrazolium bromide (MTT reagent)

ASJC Scopus subject areas

  • Materials Chemistry
  • Polymers and Plastics
  • Organic Chemistry

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