Prenatal stress in rat causes long-term spatial memory deficit and hippocampus MRI abnormality: Differential effects of postweaning enriched environment

Chun Chung Lui, Jia Yi Wang, You Lin Tain, Yu Chieh Chen, Kow Aung Chang, Ming Chi Lai, Li Tung Huang

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Prenatal stress (PS) can cause long-term hippocampus alternations in structure and plasticity in adult offspring. Enriched environment (EE) has an effect in rescuing a variety of neurological disorders. Pregnant dams were left undisturbed (prenatal control, PC) or restrained 6 h per day from days 14 to 21 (prenatal stress, PS). Control and prenatal stressed offspring rats were subjected to a standard rearing environment (SE) or an EE on postnatal days 22-120 (PC/SE PC/EE, PS/SE, and PS/EE; n = 5, each group). At ∼4 months of age, all rats underwent Morris water maze test and brain MRI examination. Hippocampi were then dissected for biochemical analyses, including, Western blot for NMDA receptor (NR) subunits and synaptophysin and RT-PCR forβ1 integrin and tissue-plasminogen activator (t-PA). MRI showed all 5 rats in the PS/SE group and 5 in the PS/EE group exhibited increased signals in bilateral hippocampus and increased T2 time in the PS/SE group. Exposure to EE treatment on postnatal days 22-120 counteracted the deficit in spatial memory and increased NR1 protein expression, but it did not affect the rate of high signals and increased T2 time, decreased NR2, synaptophysin, β1 integrin and t-PA mRNA expressions in PS adult offspring. The results of this study indicate PS in rats causes long-term spatial memory deficits and gross hippocampus pathology. Postnatal EE treatment has differential benefits in terms of spatial learning, signaling molecules, and gross hippocampus pathology.

Original languageEnglish
Pages (from-to)434-441
Number of pages8
JournalNeurochemistry International
Volume58
Issue number3
DOIs
Publication statusPublished - Feb 2011

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Long-Term Memory
Memory Disorders
Hippocampus
Synaptophysin
Tissue Plasminogen Activator
Integrins
Spatial Memory
Pathology
Nervous System Diseases
N-Methyl-D-Aspartate Receptors
Western Blotting

Keywords

  • Enriched environment
  • Magnetic resonance image
  • NMDA receptors
  • Prenatal stress
  • Spatial learning
  • T2 relaxation time

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Cell Biology

Cite this

Prenatal stress in rat causes long-term spatial memory deficit and hippocampus MRI abnormality : Differential effects of postweaning enriched environment. / Lui, Chun Chung; Wang, Jia Yi; Tain, You Lin; Chen, Yu Chieh; Chang, Kow Aung; Lai, Ming Chi; Huang, Li Tung.

In: Neurochemistry International, Vol. 58, No. 3, 02.2011, p. 434-441.

Research output: Contribution to journalArticle

Lui, Chun Chung ; Wang, Jia Yi ; Tain, You Lin ; Chen, Yu Chieh ; Chang, Kow Aung ; Lai, Ming Chi ; Huang, Li Tung. / Prenatal stress in rat causes long-term spatial memory deficit and hippocampus MRI abnormality : Differential effects of postweaning enriched environment. In: Neurochemistry International. 2011 ; Vol. 58, No. 3. pp. 434-441.
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