Preliminary study of ethylene glycol-induced alanine-glyoxylate aminotransferase 2 expression in rat kidney

Wen Chi Chen, Hsin Ping Liu, Hsi Chin Wu, Chou Huang Tsai, Huey Yi Chen, Hsin Yi Chen, Fuu Jen Tsai, Chiao Hui Chang, Po Len Liu, Feng Yen Lin, Yi Chun Chang, Wei Yong Lin, Yung Hsiang Chen

Research output: Contribution to journalArticle

Abstract

Ethylene glycol (EG), a frequently used lithogenic agent, causes calcium oxalate crystal deposits in the renal cortex and causes death if over-dosage. In this study, we compared the protein expression pattern of rat renal lithiasis induced by EG with that of normal renal tissues using two-dimensional electrophoresis (2-DE). Sixteen male Sprague-Dawley rats were divided into 2 groups. In control group (n = 8), rats were fed with normal drinking water; in EG group (n = 8), rats were fed with 0.75% EG in drinking water. After 4 weeks, rats fed with EG had calcium oxalate deposited in renal cortex. The renal cortex proteins were extracted and isolated by 2-DE. Comparative analysis of the respective spot patterns on 2-DE were identified by matrix-assisted laser desorption ionization-time of flight-mass spectrometry technique. Among them, overexpression of alanine-glyoxylate aminotransferase 2 (AGT2) in rat renal lithiasis was confirmed by realtime polymerase chain reaction analysis. The data show that overexpression of AGT2 was involved in EG-induced rat renal lithiasis. Since AGT2 is a key enzyme in glyoxylate detoxification, our preliminary results suggest AGT2 may play some important roles in response to EG-induced stone formation.

Original languageEnglish
Pages (from-to)129-135
Number of pages7
JournalCurrent Urology
Volume3
Issue number3
DOIs
Publication statusPublished - Nov 2009

Fingerprint

Ethylene Glycol
Kidney
Lithiasis
Calcium Oxalate
Drinking Water
Alanine-glyoxylate transaminase
Sprague Dawley Rats
Electrophoresis
Cause of Death
Mass Spectrometry
Proteins
Lasers
Polymerase Chain Reaction
Control Groups
Enzymes

Keywords

  • Alanine-glyoxylate aminotransferase
  • Calcium oxalate
  • Ethylene glycol
  • Mass spectroscopy
  • Two-dimensional electrophoresis
  • Urolithiasis

ASJC Scopus subject areas

  • Oncology
  • Reproductive Medicine
  • Urology

Cite this

Chen, W. C., Liu, H. P., Wu, H. C., Tsai, C. H., Chen, H. Y., Chen, H. Y., ... Chen, Y. H. (2009). Preliminary study of ethylene glycol-induced alanine-glyoxylate aminotransferase 2 expression in rat kidney. Current Urology, 3(3), 129-135. https://doi.org/10.1159/000253370

Preliminary study of ethylene glycol-induced alanine-glyoxylate aminotransferase 2 expression in rat kidney. / Chen, Wen Chi; Liu, Hsin Ping; Wu, Hsi Chin; Tsai, Chou Huang; Chen, Huey Yi; Chen, Hsin Yi; Tsai, Fuu Jen; Chang, Chiao Hui; Liu, Po Len; Lin, Feng Yen; Chang, Yi Chun; Lin, Wei Yong; Chen, Yung Hsiang.

In: Current Urology, Vol. 3, No. 3, 11.2009, p. 129-135.

Research output: Contribution to journalArticle

Chen, WC, Liu, HP, Wu, HC, Tsai, CH, Chen, HY, Chen, HY, Tsai, FJ, Chang, CH, Liu, PL, Lin, FY, Chang, YC, Lin, WY & Chen, YH 2009, 'Preliminary study of ethylene glycol-induced alanine-glyoxylate aminotransferase 2 expression in rat kidney', Current Urology, vol. 3, no. 3, pp. 129-135. https://doi.org/10.1159/000253370
Chen, Wen Chi ; Liu, Hsin Ping ; Wu, Hsi Chin ; Tsai, Chou Huang ; Chen, Huey Yi ; Chen, Hsin Yi ; Tsai, Fuu Jen ; Chang, Chiao Hui ; Liu, Po Len ; Lin, Feng Yen ; Chang, Yi Chun ; Lin, Wei Yong ; Chen, Yung Hsiang. / Preliminary study of ethylene glycol-induced alanine-glyoxylate aminotransferase 2 expression in rat kidney. In: Current Urology. 2009 ; Vol. 3, No. 3. pp. 129-135.
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