Preharvest hematopoietic progenitor cell counts predict CD34+ cell yields in granulocyte-colony-stimulating factor-mobilized peripheral blood stem cell harvest in healthy donors

Shang Hsien Yang, Tso F. Wang, His Hsiu Tsai, Teng Y. Lin, Shu H. Wen, Shu Huey Chen

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Background: The hematopoietic progenitor cell (HPC) count measured by the Sysmex hematology analyzer can determine the timing for leukapheresis in autologous peripheral blood stem cell (PBSC) harvest. We evaluated whether a HPC count could predict CD34+ cell yield in healthy, unrelated donors after granulocyte-colony-stimulating factor mobilization. Study Design And Methods: A total of 117 healthy donors underwent 161 PBSC leukapheresis procedures in our institution. The HPCs and CD34+ cells were identified by an automated hematology analyzer and flow cytometry, respectively. Using Spearman's rank test, we evaluated the relationships between preharvest HPCs, CD34+ cell counts, and CD34+ cell yields in the apheresis product. A receiver operating characteristic (ROC) curve analysis was used to identify the cutoff value of HPC for adequate mobilization and harvest yield. Results: The HPC count had a moderate correlation with the preharvest CD34+ cell count (r = 0.502, p <0.001), and an HPC count of more than 21.3 × 106/L could exclude poor mobilization (6 CD34+ cells/L) with sensitivity and specificity of 89.2 and 83.3%. However, the relationship between HPC count and CD34+ cell yield was not marked (r = 0.321, p <0.001). The area under the curve for HPCs was significantly smaller than the preharvest CD34+ cell count on the ROC curve for predicting adequate harvest yield (>10 × 10 6 CD34+ cells/L of processed blood volume, 0.678 vs. 0.850, p = 0.001). Conclusion: Although the preapheresis HPC count could predict mobilization in healthy donors before leukapheresis, it may not be a superior index for predicting CD34+ cell yield compared with the preharvest CD34+ cell count.

Original languageEnglish
Pages (from-to)1088-1095
Number of pages8
JournalTransfusion
Volume50
Issue number5
DOIs
Publication statusPublished - May 2010
Externally publishedYes

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Granulocyte Colony-Stimulating Factor
Hematopoietic Stem Cells
Cell Count
Tissue Donors
Leukapheresis
Hematology
Unrelated Donors
Blood Component Removal
Peripheral Blood Stem Cells
Blood Volume
ROC Curve
Flow Cytometry

ASJC Scopus subject areas

  • Immunology
  • Hematology
  • Immunology and Allergy

Cite this

Preharvest hematopoietic progenitor cell counts predict CD34+ cell yields in granulocyte-colony-stimulating factor-mobilized peripheral blood stem cell harvest in healthy donors. / Yang, Shang Hsien; Wang, Tso F.; Tsai, His Hsiu; Lin, Teng Y.; Wen, Shu H.; Chen, Shu Huey.

In: Transfusion, Vol. 50, No. 5, 05.2010, p. 1088-1095.

Research output: Contribution to journalArticle

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title = "Preharvest hematopoietic progenitor cell counts predict CD34+ cell yields in granulocyte-colony-stimulating factor-mobilized peripheral blood stem cell harvest in healthy donors",
abstract = "Background: The hematopoietic progenitor cell (HPC) count measured by the Sysmex hematology analyzer can determine the timing for leukapheresis in autologous peripheral blood stem cell (PBSC) harvest. We evaluated whether a HPC count could predict CD34+ cell yield in healthy, unrelated donors after granulocyte-colony-stimulating factor mobilization. Study Design And Methods: A total of 117 healthy donors underwent 161 PBSC leukapheresis procedures in our institution. The HPCs and CD34+ cells were identified by an automated hematology analyzer and flow cytometry, respectively. Using Spearman's rank test, we evaluated the relationships between preharvest HPCs, CD34+ cell counts, and CD34+ cell yields in the apheresis product. A receiver operating characteristic (ROC) curve analysis was used to identify the cutoff value of HPC for adequate mobilization and harvest yield. Results: The HPC count had a moderate correlation with the preharvest CD34+ cell count (r = 0.502, p <0.001), and an HPC count of more than 21.3 × 106/L could exclude poor mobilization (6 CD34+ cells/L) with sensitivity and specificity of 89.2 and 83.3{\%}. However, the relationship between HPC count and CD34+ cell yield was not marked (r = 0.321, p <0.001). The area under the curve for HPCs was significantly smaller than the preharvest CD34+ cell count on the ROC curve for predicting adequate harvest yield (>10 × 10 6 CD34+ cells/L of processed blood volume, 0.678 vs. 0.850, p = 0.001). Conclusion: Although the preapheresis HPC count could predict mobilization in healthy donors before leukapheresis, it may not be a superior index for predicting CD34+ cell yield compared with the preharvest CD34+ cell count.",
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T1 - Preharvest hematopoietic progenitor cell counts predict CD34+ cell yields in granulocyte-colony-stimulating factor-mobilized peripheral blood stem cell harvest in healthy donors

AU - Yang, Shang Hsien

AU - Wang, Tso F.

AU - Tsai, His Hsiu

AU - Lin, Teng Y.

AU - Wen, Shu H.

AU - Chen, Shu Huey

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N2 - Background: The hematopoietic progenitor cell (HPC) count measured by the Sysmex hematology analyzer can determine the timing for leukapheresis in autologous peripheral blood stem cell (PBSC) harvest. We evaluated whether a HPC count could predict CD34+ cell yield in healthy, unrelated donors after granulocyte-colony-stimulating factor mobilization. Study Design And Methods: A total of 117 healthy donors underwent 161 PBSC leukapheresis procedures in our institution. The HPCs and CD34+ cells were identified by an automated hematology analyzer and flow cytometry, respectively. Using Spearman's rank test, we evaluated the relationships between preharvest HPCs, CD34+ cell counts, and CD34+ cell yields in the apheresis product. A receiver operating characteristic (ROC) curve analysis was used to identify the cutoff value of HPC for adequate mobilization and harvest yield. Results: The HPC count had a moderate correlation with the preharvest CD34+ cell count (r = 0.502, p <0.001), and an HPC count of more than 21.3 × 106/L could exclude poor mobilization (6 CD34+ cells/L) with sensitivity and specificity of 89.2 and 83.3%. However, the relationship between HPC count and CD34+ cell yield was not marked (r = 0.321, p <0.001). The area under the curve for HPCs was significantly smaller than the preharvest CD34+ cell count on the ROC curve for predicting adequate harvest yield (>10 × 10 6 CD34+ cells/L of processed blood volume, 0.678 vs. 0.850, p = 0.001). Conclusion: Although the preapheresis HPC count could predict mobilization in healthy donors before leukapheresis, it may not be a superior index for predicting CD34+ cell yield compared with the preharvest CD34+ cell count.

AB - Background: The hematopoietic progenitor cell (HPC) count measured by the Sysmex hematology analyzer can determine the timing for leukapheresis in autologous peripheral blood stem cell (PBSC) harvest. We evaluated whether a HPC count could predict CD34+ cell yield in healthy, unrelated donors after granulocyte-colony-stimulating factor mobilization. Study Design And Methods: A total of 117 healthy donors underwent 161 PBSC leukapheresis procedures in our institution. The HPCs and CD34+ cells were identified by an automated hematology analyzer and flow cytometry, respectively. Using Spearman's rank test, we evaluated the relationships between preharvest HPCs, CD34+ cell counts, and CD34+ cell yields in the apheresis product. A receiver operating characteristic (ROC) curve analysis was used to identify the cutoff value of HPC for adequate mobilization and harvest yield. Results: The HPC count had a moderate correlation with the preharvest CD34+ cell count (r = 0.502, p <0.001), and an HPC count of more than 21.3 × 106/L could exclude poor mobilization (6 CD34+ cells/L) with sensitivity and specificity of 89.2 and 83.3%. However, the relationship between HPC count and CD34+ cell yield was not marked (r = 0.321, p <0.001). The area under the curve for HPCs was significantly smaller than the preharvest CD34+ cell count on the ROC curve for predicting adequate harvest yield (>10 × 10 6 CD34+ cells/L of processed blood volume, 0.678 vs. 0.850, p = 0.001). Conclusion: Although the preapheresis HPC count could predict mobilization in healthy donors before leukapheresis, it may not be a superior index for predicting CD34+ cell yield compared with the preharvest CD34+ cell count.

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