Abstract
DNA polymerase fingerprint analysis (DPFA) was employed for identifying DNA-carcinogen adduct formation in the human p53 and lac gene sequence. Two 'hot regions' at codons 223-250 and 257-283 of the p53 gene were easily attacked by nitroso-2-acetylaminofluorene or acetoxy-2-acetylaminofluorene. However, the promutational lesions in lac gene were rather randomly distributed. The chemical treated plasmid (pUC 19) which contains lac gene were transfected into Escherichia coli JM109 cells and the induced lac gene mutants were selected with X-Gal plate as indicated by the appearance of white colonies. No mutational hot regions were found in the lac gene.
| Original language | English |
|---|---|
| Pages (from-to) | 39-47 |
| Number of pages | 9 |
| Journal | Cancer Letters |
| Volume | 95 |
| Issue number | 1-2 |
| DOIs | |
| Publication status | Published - Aug 16 1995 |
| Externally published | Yes |
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Keywords
- N-Acetoxy-2-acetylaminofluorene (N-AcO-AAF)
- Nitroso-2-acetylaminofluorene (NO-AAF)
- p53 gene
ASJC Scopus subject areas
- Cancer Research
- Oncology
- Molecular Biology
Cite this
Preferential promutagenic lesions at exons 7-8 of human p53 genomic DNA induced by the direct-acting hepatocarcinogens N-nitroso-2-acetylaminofluorene and N-acetoxy-2-acetylaminofluorene. / Ho, Yuan Soon; Lin, Jen Kun.
In: Cancer Letters, Vol. 95, No. 1-2, 16.08.1995, p. 39-47.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Preferential promutagenic lesions at exons 7-8 of human p53 genomic DNA induced by the direct-acting hepatocarcinogens N-nitroso-2-acetylaminofluorene and N-acetoxy-2-acetylaminofluorene
AU - Ho, Yuan Soon
AU - Lin, Jen Kun
PY - 1995/8/16
Y1 - 1995/8/16
N2 - DNA polymerase fingerprint analysis (DPFA) was employed for identifying DNA-carcinogen adduct formation in the human p53 and lac gene sequence. Two 'hot regions' at codons 223-250 and 257-283 of the p53 gene were easily attacked by nitroso-2-acetylaminofluorene or acetoxy-2-acetylaminofluorene. However, the promutational lesions in lac gene were rather randomly distributed. The chemical treated plasmid (pUC 19) which contains lac gene were transfected into Escherichia coli JM109 cells and the induced lac gene mutants were selected with X-Gal plate as indicated by the appearance of white colonies. No mutational hot regions were found in the lac gene.
AB - DNA polymerase fingerprint analysis (DPFA) was employed for identifying DNA-carcinogen adduct formation in the human p53 and lac gene sequence. Two 'hot regions' at codons 223-250 and 257-283 of the p53 gene were easily attacked by nitroso-2-acetylaminofluorene or acetoxy-2-acetylaminofluorene. However, the promutational lesions in lac gene were rather randomly distributed. The chemical treated plasmid (pUC 19) which contains lac gene were transfected into Escherichia coli JM109 cells and the induced lac gene mutants were selected with X-Gal plate as indicated by the appearance of white colonies. No mutational hot regions were found in the lac gene.
KW - N-Acetoxy-2-acetylaminofluorene (N-AcO-AAF)
KW - Nitroso-2-acetylaminofluorene (NO-AAF)
KW - p53 gene
UR - http://www.scopus.com/inward/record.url?scp=0029155299&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0029155299&partnerID=8YFLogxK
U2 - 10.1016/0304-3835(95)03863-R
DO - 10.1016/0304-3835(95)03863-R
M3 - Article
C2 - 7656242
AN - SCOPUS:0029155299
VL - 95
SP - 39
EP - 47
JO - Cancer Letters
JF - Cancer Letters
SN - 0304-3835
IS - 1-2
ER -