Preferential promutagenic lesions at exons 7-8 of human p53 genomic DNA induced by the direct-acting hepatocarcinogens N-nitroso-2-acetylaminofluorene and N-acetoxy-2-acetylaminofluorene

Yuan Soon Ho; Jen Kun Lin

doi:10.1016/0304-3835(95)03863-R

Preferential promutagenic lesions at exons 7-8 of human p53 genomic DNA induced by the direct-acting hepatocarcinogens N-nitroso-2-acetylaminofluorene and N-acetoxy-2-acetylaminofluorene

Research output: Contribution to journal › Article › peer-review

Abstract

DNA polymerase fingerprint analysis (DPFA) was employed for identifying DNA-carcinogen adduct formation in the human p53 and lac gene sequence. Two 'hot regions' at codons 223-250 and 257-283 of the p53 gene were easily attacked by nitroso-2-acetylaminofluorene or acetoxy-2-acetylaminofluorene. However, the promutational lesions in lac gene were rather randomly distributed. The chemical treated plasmid (pUC 19) which contains lac gene were transfected into Escherichia coli JM109 cells and the induced lac gene mutants were selected with X-Gal plate as indicated by the appearance of white colonies. No mutational hot regions were found in the lac gene.

Original language	English
Pages (from-to)	39-47
Number of pages	9
Journal	Cancer Letters
Volume	95
Issue number	1-2
DOIs	https://doi.org/10.1016/0304-3835(95)03863-R
Publication status	Published - Aug 16 1995
Externally published	Yes

Keywords

N-Acetoxy-2-acetylaminofluorene (N-AcO-AAF)
Nitroso-2-acetylaminofluorene (NO-AAF)
p53 gene

ASJC Scopus subject areas

Cancer Research
Oncology
Molecular Biology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/0304-3835(95)03863-R

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@article{462ea75cfc0e4d709c939a266a88c9ad,

title = "Preferential promutagenic lesions at exons 7-8 of human p53 genomic DNA induced by the direct-acting hepatocarcinogens N-nitroso-2-acetylaminofluorene and N-acetoxy-2-acetylaminofluorene",

abstract = "DNA polymerase fingerprint analysis (DPFA) was employed for identifying DNA-carcinogen adduct formation in the human p53 and lac gene sequence. Two 'hot regions' at codons 223-250 and 257-283 of the p53 gene were easily attacked by nitroso-2-acetylaminofluorene or acetoxy-2-acetylaminofluorene. However, the promutational lesions in lac gene were rather randomly distributed. The chemical treated plasmid (pUC 19) which contains lac gene were transfected into Escherichia coli JM109 cells and the induced lac gene mutants were selected with X-Gal plate as indicated by the appearance of white colonies. No mutational hot regions were found in the lac gene.",

keywords = "N-Acetoxy-2-acetylaminofluorene (N-AcO-AAF), Nitroso-2-acetylaminofluorene (NO-AAF), p53 gene",

author = "Ho, {Yuan Soon} and Lin, {Jen Kun}",

year = "1995",

month = aug,

day = "16",

doi = "10.1016/0304-3835(95)03863-R",

language = "English",

volume = "95",

pages = "39--47",

journal = "Cancer Letters",

issn = "0304-3835",

publisher = "Elsevier Ireland Ltd",

number = "1-2",

}

TY - JOUR

T1 - Preferential promutagenic lesions at exons 7-8 of human p53 genomic DNA induced by the direct-acting hepatocarcinogens N-nitroso-2-acetylaminofluorene and N-acetoxy-2-acetylaminofluorene

AU - Ho, Yuan Soon

AU - Lin, Jen Kun

PY - 1995/8/16

Y1 - 1995/8/16

N2 - DNA polymerase fingerprint analysis (DPFA) was employed for identifying DNA-carcinogen adduct formation in the human p53 and lac gene sequence. Two 'hot regions' at codons 223-250 and 257-283 of the p53 gene were easily attacked by nitroso-2-acetylaminofluorene or acetoxy-2-acetylaminofluorene. However, the promutational lesions in lac gene were rather randomly distributed. The chemical treated plasmid (pUC 19) which contains lac gene were transfected into Escherichia coli JM109 cells and the induced lac gene mutants were selected with X-Gal plate as indicated by the appearance of white colonies. No mutational hot regions were found in the lac gene.

AB - DNA polymerase fingerprint analysis (DPFA) was employed for identifying DNA-carcinogen adduct formation in the human p53 and lac gene sequence. Two 'hot regions' at codons 223-250 and 257-283 of the p53 gene were easily attacked by nitroso-2-acetylaminofluorene or acetoxy-2-acetylaminofluorene. However, the promutational lesions in lac gene were rather randomly distributed. The chemical treated plasmid (pUC 19) which contains lac gene were transfected into Escherichia coli JM109 cells and the induced lac gene mutants were selected with X-Gal plate as indicated by the appearance of white colonies. No mutational hot regions were found in the lac gene.

KW - N-Acetoxy-2-acetylaminofluorene (N-AcO-AAF)

KW - Nitroso-2-acetylaminofluorene (NO-AAF)

KW - p53 gene

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DO - 10.1016/0304-3835(95)03863-R

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C2 - 7656242

AN - SCOPUS:0029155299

VL - 95

SP - 39

EP - 47

JO - Cancer Letters

JF - Cancer Letters

SN - 0304-3835

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ER -

Preferential promutagenic lesions at exons 7-8 of human p53 genomic DNA induced by the direct-acting hepatocarcinogens N-nitroso-2-acetylaminofluorene and N-acetoxy-2-acetylaminofluorene

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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