Predictive value of p16/Ki-67 immunocytochemistry for triage of women with abnormal Papanicolaou test in cervical cancer screening: A systematic review and meta-Analysis

Cheng Chieh Chen, Lee Wen Huang, Chyi Huey Bai, Chin Cheng Lee

Research output: Contribution to journalReview article

9 Citations (Scopus)

Abstract

Background: The Papanicolaou (Pap) test is one screening strategy used to prevent cervical cancer in developed countries. The p16/Ki-67 immunocytochemistry is a triage test performed on Pap smears in women with atypical squamous cells of undetermined significance (ASCUS) or low grade squamous intraepithelial lesion. Ob jective: Our objective was to review studies investigating the diagnostic performance of p16/Ki-67 dual stain for triage of women with abnormal Pap tests. Design: We conducted a systematic review and meta-Analysis of diagnostic test accuracy studies. Settings: We followed the protocol of systematic review of diagnostic accuracy studies. Patients AND Methods: We searched PubMed, The Cochrane Library, BioMed Central, and ClinicalTrials.gov for relevant studies. We included research that assessed the accuracy of p16/Ki-67 dual stain and high risk human papillomavirus testing for triage of abnormal Pap smears. Review articles and studies that provided insufficient data to construct 2×2 tables were excluded. Data synthesis was conducted using a random-effects model. Main Outcome Measures: Sensitivity and specificity. Results: In seven studies encompassing 2628 patients, the pooled sensitivity and specificity of p16/Ki-67 for triage of abnormal Pap smear results were 0.91 (95% CI, 0.89 to 0.93) and 0.64 (95% CI, 0.62 to 0.66), respectively. No study used a case-control design. A subgroup analysis involving liquid-based cytology showed a sensitivity of 0.91 (95%CI, 0.89 to 0.93) and specificity of 0.64 (95%CI, 0.61 to 0.66). Conclusions: Our meta-Analysis of p16/Ki-67 dual stain studies showed that the test achieved high sensitivity and moderate specificity for p16/Ki-67 immunocytochemistry for high-grade squamous intraepithelial lesion and cervical cancer. We suggest that p16/Ki-67 dual stain might be a reliable ancillary method identifying high-grade squamous intraepithelial lesions in women with abnormal Pap tests. Limitations: No study in the meta-Analysis examined the accuracy of the p16/Ki-67 dual stain for interpretation of glandular neoplasms.

Original languageEnglish
Pages (from-to)245-251
Number of pages7
JournalAnnals of Saudi Medicine
Volume36
Issue number4
DOIs
Publication statusPublished - Jul 1 2016

ASJC Scopus subject areas

  • Medicine(all)

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