Preconcentration enhanced immunoassay for protein sensing

Yu Jui Fan, Chih Zong Deng, Horn Jiunn Sheen

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

This study reports on nanobeads immunosensing technique integrated with a nanofluidic preconcentration device to develop a rapid protein biosensor with high sensitivity and high selectivity. 300 nanobeads modified with antibodies were mixed with antigen and then pumped into a nanofluidic preconcentration device. After isolating the concentrated antigens and antibody-coated nanobeads, the micro-particle tracking velocimetry was used to measure the Brownian diffusion of the nanobeads in real-time to determine the antigen concentration. From this preconcentration experiment, we demonstrated that the rapid and high-sensitivity detection of biomarker can have a detection limit of 50 pg/ml in 20 min.

Original languageEnglish
Title of host publication21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017
PublisherChemical and Biological Microsystems Society
Pages435-436
Number of pages2
ISBN (Electronic)9780692941836
Publication statusPublished - Jan 1 2020
Event21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017 - Savannah, United States
Duration: Oct 22 2017Oct 26 2017

Publication series

Name21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017

Conference

Conference21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017
CountryUnited States
CitySavannah
Period10/22/1710/26/17

Keywords

  • Brownian motion
  • Micro-PTV
  • Nanobeads
  • Nanofluidic preconcentrator

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering

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  • Cite this

    Fan, Y. J., Deng, C. Z., & Sheen, H. J. (2020). Preconcentration enhanced immunoassay for protein sensing. In 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017 (pp. 435-436). (21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017). Chemical and Biological Microsystems Society.