PPARδ-mediated p21/p27 induction via increased CREB-binding protein nuclear translocation in beraprost-induced antiproliferation of murine aortic smooth muscle cells

Yuh Mou Sue, Chih Peng Chung, Heng Lin, Ying Chou, Chih Yu Jen, Hsiao Fen Li, Chih Cheng Chang, Shu Hui Juan

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25 Citations (Scopus)

Abstract

We previously showed that an increase in the peroxisome proliferator-activated receptor-δ (PPARδ), together with subsequent induction of inducible nitric oxide synthase (iNOS) by beraprost (BPS), inhibits aortic smooth muscle cell proliferation. Herein, we delineated the mechanisms of the antiproliferative effects of BPS through the induction of p21/p27. BPS concentration dependently induced the p21/p27 promoter-and consensus cAMP-responsive element (CRE)-driven luciferase activities, which were significantly suppressed by blocking PPARδ activation. Surprisingly, other than altering the CRE-binding protein (CREB), BPS-mediated PPARδ activation increased nuclear localization of the CREB-binding protein (CBP), a coactivator, which was further confirmed by chromatin immunoprecipitation. Furthermore, novel functional PPAR-responsive elements (PPREs) next to CREs in the rat p21/p27 promoter regions were identified, where PPARδ interacted with CREB through CBP recruitment. BPS-mediated suppression of restenosis in mice with angioplasty was associated with p21/p27 induction. Herein, we demonstrate for the first time that BPS-mediated PPARδ activation enhances transcriptional activation of p21/p27 by increasing CBP nuclear translocation, which contributes to the vasoprotective action of BPS.

Original languageEnglish
JournalAmerican Journal of Physiology - Cell Physiology
Volume297
Issue number2
DOIs
Publication statusPublished - Aug 2009

Fingerprint

beraprost
Peroxisome Proliferator-Activated Receptors
Protein Transport
Protein Binding
Smooth Muscle Myocytes
Carrier Proteins
CREB-Binding Protein
Chromatin Immunoprecipitation
Nitric Oxide Synthase Type II
Luciferases
Angioplasty
Genetic Promoter Regions
Transcriptional Activation

Keywords

  • Antiproliferation
  • cAMP-responsive element
  • cAMP-responsive element-binding protein
  • cAMP-responsive element-binding protein-binding protein

ASJC Scopus subject areas

  • Cell Biology
  • Physiology
  • Medicine(all)

Cite this

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title = "PPARδ-mediated p21/p27 induction via increased CREB-binding protein nuclear translocation in beraprost-induced antiproliferation of murine aortic smooth muscle cells",
abstract = "We previously showed that an increase in the peroxisome proliferator-activated receptor-δ (PPARδ), together with subsequent induction of inducible nitric oxide synthase (iNOS) by beraprost (BPS), inhibits aortic smooth muscle cell proliferation. Herein, we delineated the mechanisms of the antiproliferative effects of BPS through the induction of p21/p27. BPS concentration dependently induced the p21/p27 promoter-and consensus cAMP-responsive element (CRE)-driven luciferase activities, which were significantly suppressed by blocking PPARδ activation. Surprisingly, other than altering the CRE-binding protein (CREB), BPS-mediated PPARδ activation increased nuclear localization of the CREB-binding protein (CBP), a coactivator, which was further confirmed by chromatin immunoprecipitation. Furthermore, novel functional PPAR-responsive elements (PPREs) next to CREs in the rat p21/p27 promoter regions were identified, where PPARδ interacted with CREB through CBP recruitment. BPS-mediated suppression of restenosis in mice with angioplasty was associated with p21/p27 induction. Herein, we demonstrate for the first time that BPS-mediated PPARδ activation enhances transcriptional activation of p21/p27 by increasing CBP nuclear translocation, which contributes to the vasoprotective action of BPS.",
keywords = "Antiproliferation, cAMP-responsive element, cAMP-responsive element-binding protein, cAMP-responsive element-binding protein-binding protein",
author = "Sue, {Yuh Mou} and Chung, {Chih Peng} and Heng Lin and Ying Chou and Jen, {Chih Yu} and Li, {Hsiao Fen} and Chang, {Chih Cheng} and Juan, {Shu Hui}",
year = "2009",
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T1 - PPARδ-mediated p21/p27 induction via increased CREB-binding protein nuclear translocation in beraprost-induced antiproliferation of murine aortic smooth muscle cells

AU - Sue, Yuh Mou

AU - Chung, Chih Peng

AU - Lin, Heng

AU - Chou, Ying

AU - Jen, Chih Yu

AU - Li, Hsiao Fen

AU - Chang, Chih Cheng

AU - Juan, Shu Hui

PY - 2009/8

Y1 - 2009/8

N2 - We previously showed that an increase in the peroxisome proliferator-activated receptor-δ (PPARδ), together with subsequent induction of inducible nitric oxide synthase (iNOS) by beraprost (BPS), inhibits aortic smooth muscle cell proliferation. Herein, we delineated the mechanisms of the antiproliferative effects of BPS through the induction of p21/p27. BPS concentration dependently induced the p21/p27 promoter-and consensus cAMP-responsive element (CRE)-driven luciferase activities, which were significantly suppressed by blocking PPARδ activation. Surprisingly, other than altering the CRE-binding protein (CREB), BPS-mediated PPARδ activation increased nuclear localization of the CREB-binding protein (CBP), a coactivator, which was further confirmed by chromatin immunoprecipitation. Furthermore, novel functional PPAR-responsive elements (PPREs) next to CREs in the rat p21/p27 promoter regions were identified, where PPARδ interacted with CREB through CBP recruitment. BPS-mediated suppression of restenosis in mice with angioplasty was associated with p21/p27 induction. Herein, we demonstrate for the first time that BPS-mediated PPARδ activation enhances transcriptional activation of p21/p27 by increasing CBP nuclear translocation, which contributes to the vasoprotective action of BPS.

AB - We previously showed that an increase in the peroxisome proliferator-activated receptor-δ (PPARδ), together with subsequent induction of inducible nitric oxide synthase (iNOS) by beraprost (BPS), inhibits aortic smooth muscle cell proliferation. Herein, we delineated the mechanisms of the antiproliferative effects of BPS through the induction of p21/p27. BPS concentration dependently induced the p21/p27 promoter-and consensus cAMP-responsive element (CRE)-driven luciferase activities, which were significantly suppressed by blocking PPARδ activation. Surprisingly, other than altering the CRE-binding protein (CREB), BPS-mediated PPARδ activation increased nuclear localization of the CREB-binding protein (CBP), a coactivator, which was further confirmed by chromatin immunoprecipitation. Furthermore, novel functional PPAR-responsive elements (PPREs) next to CREs in the rat p21/p27 promoter regions were identified, where PPARδ interacted with CREB through CBP recruitment. BPS-mediated suppression of restenosis in mice with angioplasty was associated with p21/p27 induction. Herein, we demonstrate for the first time that BPS-mediated PPARδ activation enhances transcriptional activation of p21/p27 by increasing CBP nuclear translocation, which contributes to the vasoprotective action of BPS.

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