Pparα deficiency inhibits the proliferation of neuronal and glial precursors in the zebrafish central nervous system

Yen Che Hsieh, Ming Chang Chiang, Yin Cheng Huang, Tu Hsueh Yeh, Hung Yu Shih, Han Fang Liu, Hao Yuan Chen, Chien Ping Wang, Yi Chuan Cheng

Research output: Contribution to journalArticle

Abstract

Background: Many molecules and signaling pathways involved in neural development play a role in neurodegenerative diseases and brain tumor progression. Peroxisome proliferator-activated receptor (PPAR) proteins regulate the differentiation of tissues and the progression of many diseases. However, the role of these proteins in neural development is unclear. Results: We examined the function of Pparα in the neural development of zebrafish. Two duplicate paralogs for mammalian PPARA/Ppara, namely pparaa and pparab, are present in the zebrafish genome. Both pparaa and pparab are expressed in the developing central nervous system in zebrafish embryos. Inhibiting the function of Pparα by using either the PPARα/Pparα antagonist GW6471 or pparaa or pparab truncated constructs produced identical phenotypes, which were sufficient to reduce the proliferation of neuronal and glial precursor cells without affecting the formation of neural progenitors. Conclusions: We demonstrated that both Pparαa and Pparαb proteins are essential regulators of the proliferation of neuronal and glial precursors. This study provides a better understanding of the functions of PPARα/Pparα in neural development and further expands our knowledge of the potential role of PPARα/Pparα in neurological disorders and brain tumors. Developmental Dynamics 247:1264–1275, 2018.

Original languageEnglish
Pages (from-to)1264-1275
Number of pages12
JournalDevelopmental Dynamics
Volume247
Issue number12
DOIs
Publication statusPublished - Dec 1 2018

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Peroxisome Proliferator-Activated Receptors
Zebrafish
Neuroglia
Central Nervous System
Brain Neoplasms
Proteins
Nervous System Diseases
Neurodegenerative Diseases
Disease Progression
Embryonic Structures
Genome
Phenotype

Keywords

  • glial precursor
  • neural development
  • neuronal precursor
  • Pparα
  • zebrafish

ASJC Scopus subject areas

  • Developmental Biology

Cite this

Pparα deficiency inhibits the proliferation of neuronal and glial precursors in the zebrafish central nervous system. / Hsieh, Yen Che; Chiang, Ming Chang; Huang, Yin Cheng; Yeh, Tu Hsueh; Shih, Hung Yu; Liu, Han Fang; Chen, Hao Yuan; Wang, Chien Ping; Cheng, Yi Chuan.

In: Developmental Dynamics, Vol. 247, No. 12, 01.12.2018, p. 1264-1275.

Research output: Contribution to journalArticle

Hsieh, YC, Chiang, MC, Huang, YC, Yeh, TH, Shih, HY, Liu, HF, Chen, HY, Wang, CP & Cheng, YC 2018, 'Pparα deficiency inhibits the proliferation of neuronal and glial precursors in the zebrafish central nervous system', Developmental Dynamics, vol. 247, no. 12, pp. 1264-1275. https://doi.org/10.1002/dvdy.24683
Hsieh, Yen Che ; Chiang, Ming Chang ; Huang, Yin Cheng ; Yeh, Tu Hsueh ; Shih, Hung Yu ; Liu, Han Fang ; Chen, Hao Yuan ; Wang, Chien Ping ; Cheng, Yi Chuan. / Pparα deficiency inhibits the proliferation of neuronal and glial precursors in the zebrafish central nervous system. In: Developmental Dynamics. 2018 ; Vol. 247, No. 12. pp. 1264-1275.
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