Power spectral analysis of electroencephalographic desynchronization induced by cocaine in rats: Correlation with evaluation of noradrenergic neurotransmission at the medial prefrontal cortex

Alice Y.W. Chang, Terry B.J. Kuo, T. H. Tsai, C. F. Chen, Samuel H.H. Chan

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We applied continuous, on‐line and real‐time spectral analysis of electro‐ encephalographic (EEG) signals and microdialysis to evaluate the possible participation of noradrenergic neurotransmission at the medial prefrontal cortex (mPFC) in EEG desynchronization induced by cocaine. Male Sprague‐Dawley rats that were under chloral hydrate anesthesia were used. Intravenous administration of cocaine (1.5 or 3.0 mg/kg) dose‐dependently induced EEG desynchronization, as represented by a decrease in root mean square (RMS) and an increase in mean power frequency (MPF) value of the EEG signals. Power spectral analysis further revealed that whereas both doses of cocaine promoted a reduction in the α (8–13 Hz), θ (4–8 Hz), and δ (1–4 Hz) components, the lower dose of cocaine decreased, and the higher dose increased the β band (13–32 Hz). Microdialysis data indicated an elevation in extracellular concentration of norepinephrine at the mPFC that paralleled temporally and correlated positively with the maximal effect of cocaine on EEG activity. Bilateral microinjection of the selective noradrenergic neurotoxin, DSP4 (50 μg), or equimolar concentration (500 pmol) of the α1‐aladrenoceptor antagonist, prazosin, or α2‐adrenoceptor antagonist, yohimbine, into the mPFC significantly blunted the decrease in δ component (prazosin) or both β and θ components (DSP4 or yohimbine) of EEG activity by the lower dose of cocaine. On the other hand, the same pretreatments appreciably antagonized the increase in β band by cocaine at 3.0 mg/kg. The potency of the antagonism by yohimbine, however, was higher than prazosin. These results suggest that cocaine may elicit EEG desynchronization via noradrenergic neuro‐ transmission, and that α2‐adrenoceptors, and to a lesser extent, α1‐adrenoceptors, at the mPFC may be involved in the subtle dose‐dependent changes in individual EEG spectral components. © 1995 Wiley‐Liss, Inc.

Original languageEnglish
Pages (from-to)149-157
Number of pages9
Issue number2
Publication statusPublished - 1995
Externally publishedYes



  • al‐and a2‐adrenoceptors
  • DSP4
  • Microdialysis
  • Norepinephrine
  • Prazosin
  • Yohimbine

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience

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