Potential therapeutic effect of curcumin, a natural mTOR inhibitor, in tuberous sclerosis complex

Chu Jen Kuo, Chi Chen Huang, Szu Yi Chou, Yu Chun Lo, Tzu Jen Kao, Nai Kuei Huang, Connie Lin, Hsin Chuan Lin, Hui Ching Lin, Yi Chao Lee

Research output: Contribution to journalArticle

Abstract

Background: Curcumin is a polyphenol natural product of the plant Curcuma longa. Recent studies suggest that curcumin inhibit mTOR activity in vitro, which prompts us to investigate curcumin function as a new class of mTOR inhibitor suitable for tuberous sclerosis complex (TSC) treatment. Purpose: We aim to investigate the efficacy of curcumin in the treatment of TSC related manifestations in animal model. Study Design: Solid lipid curcumin particle (SLCP), a novel curcumin formulation, was used to treat TSC related manifestations in Tsc2 knockout mice. Methods: The novel object recognition test was used to analyze the recognition memory function. The long-term potentiation was studied using electrophysiological analysis. Western blotting was used to assess the protein expression and activation status. Results: Recognition memory deficit began as early as 4 weeks of age in both male and female Tsc2+/− mice. Oral administration with SLCP activates AMPK activity and inhibits mTOR activity in the brain tissue of Tsc2+/− mice, and can rescue the electrophysiological abnormality and object recognition memory loss in the mice. Conclusions: Our results suggest that SLCP could be an effective treatment for TSC patients.

LanguageEnglish
Pages132-139
Number of pages8
JournalPhytomedicine
Volume54
DOIs
Publication statusPublished - Feb 15 2019

Fingerprint

Tuberous Sclerosis
Curcumin
Therapeutic Uses
Memory Disorders
Lipids
Curcuma
AMP-Activated Protein Kinases
Long-Term Potentiation
Polyphenols
Biological Products
Knockout Mice
Oral Administration
Therapeutics
Animal Models
Western Blotting
Recognition (Psychology)
Brain

Keywords

  • Curcumin
  • Solid lipid curcumin particle
  • Tuberous sclerosis complex

ASJC Scopus subject areas

  • Molecular Medicine
  • Pharmacology
  • Pharmaceutical Science
  • Drug Discovery
  • Complementary and alternative medicine

Cite this

Potential therapeutic effect of curcumin, a natural mTOR inhibitor, in tuberous sclerosis complex. / Kuo, Chu Jen; Huang, Chi Chen; Chou, Szu Yi; Lo, Yu Chun; Kao, Tzu Jen; Huang, Nai Kuei; Lin, Connie; Lin, Hsin Chuan; Lin, Hui Ching; Lee, Yi Chao.

In: Phytomedicine, Vol. 54, 15.02.2019, p. 132-139.

Research output: Contribution to journalArticle

@article{15c3980b57404628b7fff798f4126f02,
title = "Potential therapeutic effect of curcumin, a natural mTOR inhibitor, in tuberous sclerosis complex",
abstract = "Background: Curcumin is a polyphenol natural product of the plant Curcuma longa. Recent studies suggest that curcumin inhibit mTOR activity in vitro, which prompts us to investigate curcumin function as a new class of mTOR inhibitor suitable for tuberous sclerosis complex (TSC) treatment. Purpose: We aim to investigate the efficacy of curcumin in the treatment of TSC related manifestations in animal model. Study Design: Solid lipid curcumin particle (SLCP), a novel curcumin formulation, was used to treat TSC related manifestations in Tsc2 knockout mice. Methods: The novel object recognition test was used to analyze the recognition memory function. The long-term potentiation was studied using electrophysiological analysis. Western blotting was used to assess the protein expression and activation status. Results: Recognition memory deficit began as early as 4 weeks of age in both male and female Tsc2+/− mice. Oral administration with SLCP activates AMPK activity and inhibits mTOR activity in the brain tissue of Tsc2+/− mice, and can rescue the electrophysiological abnormality and object recognition memory loss in the mice. Conclusions: Our results suggest that SLCP could be an effective treatment for TSC patients.",
keywords = "Curcumin, Solid lipid curcumin particle, Tuberous sclerosis complex, Curcumin, Solid lipid curcumin particle, Tuberous sclerosis complex",
author = "Kuo, {Chu Jen} and Huang, {Chi Chen} and Chou, {Szu Yi} and Lo, {Yu Chun} and Kao, {Tzu Jen} and Huang, {Nai Kuei} and Connie Lin and Lin, {Hsin Chuan} and Lin, {Hui Ching} and Lee, {Yi Chao}",
year = "2019",
month = "2",
day = "15",
doi = "10.1016/j.phymed.2018.09.203",
language = "English",
volume = "54",
pages = "132--139",
journal = "Phytomedicine",
issn = "0944-7113",
publisher = "Urban und Fischer Verlag Jena",

}

TY - JOUR

T1 - Potential therapeutic effect of curcumin, a natural mTOR inhibitor, in tuberous sclerosis complex

AU - Kuo, Chu Jen

AU - Huang, Chi Chen

AU - Chou, Szu Yi

AU - Lo, Yu Chun

AU - Kao, Tzu Jen

AU - Huang, Nai Kuei

AU - Lin, Connie

AU - Lin, Hsin Chuan

AU - Lin, Hui Ching

AU - Lee, Yi Chao

PY - 2019/2/15

Y1 - 2019/2/15

N2 - Background: Curcumin is a polyphenol natural product of the plant Curcuma longa. Recent studies suggest that curcumin inhibit mTOR activity in vitro, which prompts us to investigate curcumin function as a new class of mTOR inhibitor suitable for tuberous sclerosis complex (TSC) treatment. Purpose: We aim to investigate the efficacy of curcumin in the treatment of TSC related manifestations in animal model. Study Design: Solid lipid curcumin particle (SLCP), a novel curcumin formulation, was used to treat TSC related manifestations in Tsc2 knockout mice. Methods: The novel object recognition test was used to analyze the recognition memory function. The long-term potentiation was studied using electrophysiological analysis. Western blotting was used to assess the protein expression and activation status. Results: Recognition memory deficit began as early as 4 weeks of age in both male and female Tsc2+/− mice. Oral administration with SLCP activates AMPK activity and inhibits mTOR activity in the brain tissue of Tsc2+/− mice, and can rescue the electrophysiological abnormality and object recognition memory loss in the mice. Conclusions: Our results suggest that SLCP could be an effective treatment for TSC patients.

AB - Background: Curcumin is a polyphenol natural product of the plant Curcuma longa. Recent studies suggest that curcumin inhibit mTOR activity in vitro, which prompts us to investigate curcumin function as a new class of mTOR inhibitor suitable for tuberous sclerosis complex (TSC) treatment. Purpose: We aim to investigate the efficacy of curcumin in the treatment of TSC related manifestations in animal model. Study Design: Solid lipid curcumin particle (SLCP), a novel curcumin formulation, was used to treat TSC related manifestations in Tsc2 knockout mice. Methods: The novel object recognition test was used to analyze the recognition memory function. The long-term potentiation was studied using electrophysiological analysis. Western blotting was used to assess the protein expression and activation status. Results: Recognition memory deficit began as early as 4 weeks of age in both male and female Tsc2+/− mice. Oral administration with SLCP activates AMPK activity and inhibits mTOR activity in the brain tissue of Tsc2+/− mice, and can rescue the electrophysiological abnormality and object recognition memory loss in the mice. Conclusions: Our results suggest that SLCP could be an effective treatment for TSC patients.

KW - Curcumin

KW - Solid lipid curcumin particle

KW - Tuberous sclerosis complex

KW - Curcumin

KW - Solid lipid curcumin particle

KW - Tuberous sclerosis complex

UR - http://www.scopus.com/inward/record.url?scp=85055732727&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85055732727&partnerID=8YFLogxK

U2 - 10.1016/j.phymed.2018.09.203

DO - 10.1016/j.phymed.2018.09.203

M3 - Article

VL - 54

SP - 132

EP - 139

JO - Phytomedicine

T2 - Phytomedicine

JF - Phytomedicine

SN - 0944-7113

ER -